Insulin overlapping in whole blood FTIR spectroscopy in blood glucose measurements

Romo-Cárdenas, Gerardo Salvador; Sanchez-Lopez, Juan de Dios; Luque, P.A.; Cosío-León, María de los Ángeles; Hipólito, Juan Iván Nieto; Vazquez-Briseno, Mabel

doi:10.1016/j.rinp.2017.03.017

Cited by 12 publications

(4 citation statements)

References 10 publications

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“…Meanwhile, absorption peaks at 1653 and 1551 cm −1 are attributed to amide I and II bands, respectively, in the insulin structure. The presence of these characteristic bands of insulin in this spectrum can confirm the successful loading of insulin in the clay with no significant denaturation [ 8 , 44 ].…”

Section: Resultsmentioning

confidence: 97%

Novel Black Seed Polysaccharide Extract-g-Poly (Acrylate) pH-Responsive Hydrogel Nanocomposites for Safe Oral Insulin Delivery: Development, In Vitro, In Vivo and Toxicological Evaluation

et al. 2022

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Oral delivery of insulin has always been a challenging task due to harsh gut environment involving variable pH and peptidase actions. Currently, no Food and Drug Administration (FDA) approved oral insulin formulation is commercially available, only intravenous (IV) or subcutaneous (SC) routes. Therefore, it is really cumbersome for diabetic patients to go through invasive approaches for insulin delivery on daily basis. In the present study, a novel pH-responsive hydrogel nanocomposite (NC) system was developed and optimized for safe oral delivery of insulin. Black seed polysaccharide extract-based hydrogel (BA hydrogel) was formulated by free radical polymerization and loaded with insulin. Blank BA hydrogel was also incorporated with insulin-loaded montmorillonite nanoclay (Ins-Mmt) to form an Ins-Mmt-BA hydrogel NC and compared with the insulin-loaded hydrogel. Swelling, sol-gel analysis and in vitro release studies proved that Ins-Mmt-BA6 hydrogel NC has the best formulation, with 96.17% maximum insulin released in 24 h. Kinetic modeling applied on insulin release data showed the Korsemeyer-Peppas model (R2 = 0.9637) as the best fit model with a super case II transport mechanism for insulin transport (n > 0.89). Energy Dispersive X-ray (EDX) Spectroscopy, Fourier Transformed Infrared (FTIR) spectroscopy and Powdered X-ray diffraction (PXRD) analysis results also confirmed successful development of a hydrogel NC with no significant denaturation of insulin. Toxicity results confirmed the safety profile and biocompatibility of the developed NC. In vivo studies showed a maximum decrease in blood glucose levels of 52.61% and percentage relative bioavailability (% RBA) of 26.3% for an Ins-Mmt-BA hydrogel NC as compared to BA hydrogels and insulin administered through the SC route.

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Section: Resultsmentioning

confidence: 97%

Novel Black Seed Polysaccharide Extract-g-Poly (Acrylate) pH-Responsive Hydrogel Nanocomposites for Safe Oral Insulin Delivery: Development, In Vitro, In Vivo and Toxicological Evaluation

et al. 2022

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“…High Low Table 1. Peak wavenumbers in the spectra and candidate components of biological tissues [6][7][8] .…”

Section: High (180) Low (<100) Blood Glucose Levelmentioning

confidence: 99%

Healthcare monitoring systems using mid-infrared and deep ultraviolet spectroscopy

Matsuura

2024

Optical Fibers and Sensors for Medical Diagnostics, Treatment, and Environmental Applications XXIV

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Spectroscopy using mid-infrared light with a wavelength of 6 to 12 microns enables high-precision analysis of proteins, lipids, and carbohydrates that constitute living organisms. Recently, mid-infrared quantum cascade lasers and semiconductor detectors operating at room temperature have emerged in this field, increasing the feasibility of compact and inexpensive healthcare devices. In the deep ultraviolet region below 300 nm wavelength, various volatile gases show particularly strong absorption, and new applications are expected to emerge with the development of LEDs and new light sources. In this paper, non-invasive analysis of blood components based on mid-infrared photoacoustic spectroscopy is reported. As examples of applications of deep ultraviolet and vacuum ultraviolet spectroscopy using hollow optical fibers as microvolume and long optical path gas cells, the results of highly accurate measurement of acetone in exhaled breath, which is useful for metabolic measurements, is presented.

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“…A serious application of FT-IR spectroscopy is blood tests in the context of analysing hormones [ 57 ], proteins [ 55 , 58 ], and glucose [ 59 ]. These are often interdisciplinary studies, drawing on methods used in computational chemistry (molecular mechanics) [ 58 ], in addition to routine methods of discriminatory analysis of results [ 55 ].…”

Section: Medicinementioning

confidence: 99%

The Analytical Possibilities of FT-IR Spectroscopy Powered by Vibrating Molecules

Koczoń

Hołaj-Krzak

Palani

et al. 2023

IJMS

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This paper discusses the state of advancement in the development of spectroscopic methods based on the use of mid (proper) infrared radiation in the context of applications in various fields of science and technology. The authors drew attention to the most important solutions specific to both spectroscopy itself (ATR technique) and chemometric data processing tools (PCA and PLS models). The objective of the current paper is to collect and consistently present information on various aspects of FT-IR spectroscopy, which is not only a well-known and well-established method but is also continuously developing. The innovative aspect of the current review is to show FT-IR’s great versatility that allows its applications to solve and explain issues from both the scientific domain (e.g., hydrogen bonds) and practical ones (e.g., technological processes, medicine, environmental protection, and food analysis). Particular attention was paid to the issue of hydrogen bonds as key non-covalent interactions, conditioning the existence of living matter and determining the number of physicochemical properties of various materials. Since the role of FT-IR spectroscopy in the field of hydrogen bond research has great significance, a historical outline of the most important qualitative and quantitative hydrogen bond theories is provided. In addition, research on selected unconventional spectral effects resulting from the substitution of protons with deuterons in hydrogen bridges is presented. The state-of-the-art and originality of the current review are that it presents a combination of uses of FT-IR spectroscopy to explain the way molecules vibrate and the effects of those vibrations on macroscopic properties, hence practical applications of given substances.

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Insulin overlapping in whole blood FTIR spectroscopy in blood glucose measurements

Cited by 12 publications

References 10 publications

Novel Black Seed Polysaccharide Extract-g-Poly (Acrylate) pH-Responsive Hydrogel Nanocomposites for Safe Oral Insulin Delivery: Development, In Vitro, In Vivo and Toxicological Evaluation

Novel Black Seed Polysaccharide Extract-g-Poly (Acrylate) pH-Responsive Hydrogel Nanocomposites for Safe Oral Insulin Delivery: Development, In Vitro, In Vivo and Toxicological Evaluation

Healthcare monitoring systems using mid-infrared and deep ultraviolet spectroscopy

The Analytical Possibilities of FT-IR Spectroscopy Powered by Vibrating Molecules

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