Insulin-loaded PLGA microparticles: flow focusing<i>versus</i>double emulsion/solvent evaporation

Cózar-Bernal, M.J.; Holgado, M.A.; Arias, José L.; Muñoz-Rubio, Inmaculada; Martín-Banderas, Lucía; Álvarez-Fuentes, Josefa; Fernández‐Arévalo, M.

doi:10.3109/02652048.2011.576786

Cited by 39 publications

(28 citation statements)

References 55 publications

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“…Moreover, as a cationic surfactant it can take the place of other possible surfactants [14]. PLLA possesses excellent biodegradable and biocompatible characteristics, and its perfect mechanical property makes it to be ideal polymer materials for applications in medicine and pharmacy [15][16][17][18][19]. In this research, chlorhexidine was applied as core material and emulsifying agent of microcapsules, poly-L-lactic acid (PLLA) was used as wall material of microcapsules.…”

Section: Introductionmentioning

confidence: 99%

PLLA microcapsules combined with silver nanoparticles and chlorhexidine acetate showing improved antibacterial effect

Zhou

Guo

et al. 2017

Materials Science and Engineering: C

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Section: Introductionmentioning

confidence: 99%

PLLA microcapsules combined with silver nanoparticles and chlorhexidine acetate showing improved antibacterial effect

Zhou

Guo

et al. 2017

Materials Science and Engineering: C

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“…Several studies have reported insulin loading in polymeric NPs after washing the NPs with water only 47-49 . However, since water wash alone is not representative of the environment that the NPs are exposed to in vivo , it is important to understand whether insulin loading is affected by the presence of ions in the buffer.…”

Section: Resultsmentioning

confidence: 99%

Design of Insulin-Loaded Nanoparticles Enabled by Multistep Control of Nanoprecipitation and Zinc Chelation

Chopra

Bertrand

Lim

et al. 2017

ACS Appl. Mater. Interfaces

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Nanoparticle (NP) carriers provide new opportunities for controlled delivery of drugs, and have potential to address challenges such as effective oral delivery of insulin. However, due to the difficulty of efficiently loading insulin and other proteins inside polymeric NPs, their use has been mostly restricted to the encapsulation of small molecules. To better understand the processes involved in encapsulation of proteins in NPs, we study how buffer conditions, ionic chelation, and preparation methods influence insulin loading in poly (lactic-co-glycolic acid)–b–poly(ethylene glycol) (PLGA-PEG) NPs. We report that, although insulin is weakly bound and easily released from the NPs in the presence of buffer ions, insulin loading can be increased by over 10-fold with the use of chelating zinc ions and by the optimization of the pH during nanoprecipitation. We further provide ways of changing synthesis parameters to control NP size while maintaining high insulin loading. These results provide a simple method to enhance insulin loading of PLGA-PEG NPs, and provide insights that may extend to other protein drug delivery systems that are subject to limited loading.

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“…The RSD was measured and the values are showed in Table 1. To summarize, RSDs for the six recovery values for levels I, III, and V of the accuracy test are less than 2.0% (acceptance criteria) [27, 29, 33]. For this reason, this study was considered validated .…”

Section: Resultsmentioning

confidence: 99%

“…The result obtained for the average area was 8097677.88 ± 132650.17 with an RSD value of 1.64. The RSD value of analysis performed was less than 2%, which demonstrates that the method is reproducible, because the introduced variations in the test have no influence on the experimental results [27, 29, 33]. …”

Section: Resultsmentioning

confidence: 99%

Development and Validation of an RP-HPLC Method for CB13 Evaluation in Several PLGA Nanoparticle Systems

Álvarez-Fuentes

Martín-Banderas

Muñoz-Rubio

et al. 2012

The Scientific World Journal

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A simple, fast, and reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed and validated for determining of a cannabinoid derivate, which displays potent antihyperalgesic activity, 1-naphthalenyl[4-(pentyloxy)-1-naphthalenyl]methanone (CB13) into PLGA nanoparticles. Separation was achieved in a C18 column using a mobile phase consisting of two solvents: solvent A, consisting of acetonitrile : water : acetic acid (75 : 23.7 : 1.3 v/v), and solvent B, consisting of acetonitrile. An isocratic method (70 : 30 v/v), with a flow rate of 1.000 mL/min, and a diode array detector were used. The developed method was precise, accurate, and linear over the concentration range of analysis with a limit of detection and a limit of quantification of 0.5 and 1.25 μg/mL, respectively. The developed method was applied to the analysis of CB13 in nanoparticles samples obtained by three different procedures (SEV, FF, and NPP) in terms of encapsulation efficiency and drug release. Nanoparticles size and size distribution were also evaluated founding that NPP method presented the most lowest particle sizes with narrow-size distribution (≈320 nm) and slightly negative zeta potential (≈−25 mV) which presumes a suitable procedure for the synthesis of PLGA-CB13 nanoparticles for oral administration.

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Insulin-loaded PLGA microparticles: flow focusingversusdouble emulsion/solvent evaporation

Abstract: Results have lead to the identification of the best formulation conditions for the engineering of insulin-loaded PLGA microparticles against diabetes.

Cited by 39 publications

References 55 publications

PLLA microcapsules combined with silver nanoparticles and chlorhexidine acetate showing improved antibacterial effect

PLLA microcapsules combined with silver nanoparticles and chlorhexidine acetate showing improved antibacterial effect

Design of Insulin-Loaded Nanoparticles Enabled by Multistep Control of Nanoprecipitation and Zinc Chelation

Development and Validation of an RP-HPLC Method for CB13 Evaluation in Several PLGA Nanoparticle Systems

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