Insulin-like system and growth regulation in the Pacific oyster Crassostrea gigas: hrIGF-1 effect on protein synthesis of mantle edge cells and expression of an homologous insulin receptor-related receptor

Gricourt, Linpa; Bonnec, Georgette; Boujard, Daniel; Mathieu, Michel; Kellner, K

doi:10.1016/s0016-6480(03)00217-x

Cited by 76 publications

(40 citation statements)

References 86 publications

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“…This superfamily of proteins was also found to be a determinant of lifespan in various animal species (Barbieri et al, 2003). Despite the availability of a cDNA encoding a tyrosine kinase insulin receptor in C. gigas (Gricourt et al, 2003) and the actual functionality of vertebrate insulin/IGF ligands in mollusks (Giard et al, 1998;Gricourt et al, 2006), the gain of a sequence encoding an endogenous insulin will definitely help to decipher the involvement of this central (neuro)endocrine factor in the coordination of growth, reproduction, and metabolism in relation with the nutritional status of the oyster. This sequencing project has also generated a number of ESTs encoding potential receptors for extracellular ligands.…”

Section: Resultsmentioning

confidence: 99%

Increasing genomic information in bivalves through new EST collections in four species: Development of new genetic markers for environmental studies and genome evolution

Tanguy

Bierne

Saavedra

et al. 2008

Gene

128

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Section: Resultsmentioning

confidence: 99%

Increasing genomic information in bivalves through new EST collections in four species: Development of new genetic markers for environmental studies and genome evolution

Tanguy

Bierne

Saavedra

et al. 2008

Gene

128

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“…An increase in 17b-estradiol has been observed during gametogenesis, suggesting its role in vitellogenesis [14], and an estrogen receptor is predominantly expressed in mature animals [15]. Moreover, several elements of the insulin signaling pathway were identified in C. gigas: an insulin-like peptide ligand (ILP) expressed in the visceral ganglia and regulated during gametogenesis [16], a C. gigas insulin receptor-related receptor (CIR) [17], Ras oncogene, phosphatase-tensin homolog deleted on chromosome (PTEN), and p70-S6 kinase [18]. However, no functional data are yet available for these endocrine and signaling molecules in reproduction, and further analyses are needed to study their function.…”

Section: Introductionmentioning

confidence: 99%

Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas1

Guévélou

Huvet

Galindo-Sánchez

et al. 2013

Biology of Reproduction

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The hermaphrodite Pacific oyster Crassostrea gigas displays a high energy allocation to reproduction. We studied the expression of AMP-activated protein kinase (AMPK) during gametogenesis in the gonad and characterized the mRNA sequences of the AMPK subunits: the AMPK alpha mRNA sequence was previously characterized; we identified AMPK beta, AMPK gamma, and mRNAs of putative AMPK-related targets following bioinformatics mining on existing genomic resources. We analyzed the mRNA expression of the AMPK alpha, beta, and gamma subunits in the gonads of male and female oysters through a reproductive cycle, and we quantified the mRNA expression of genes belonging to fatty acid and glucose metabolism. AMPK alpha mRNA levels were more abundant in males at the first stage of gametogenesis, when mitotic activity and the differentiation of germinal cells occur, and were always more abundant in males than in females. Some targets of fatty acid and glucose metabolism appeared to be correlated with the expression of AMPK subunits at the mRNA level. We then analyzed the sex-specific AMPK activity by measuring the phosphorylation of the catalytic AMPK alpha protein and its expression at the protein level. Both the amount of AMPK alpha protein and threonine 172 phosphorylation appeared to be almost totally inhibited in mature female gonads at stage 3, at the time when accumulation of reserves in oocytes was promoted, while it remained at a high level in mature spermatozoa. Its activation might play a sex-dependent role in the management of energy during gametogenesis in oyster.

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“…In M. galloprovincialis, anti-Anodonta insulin immunoreactivity was observed in the cerebral ganglia, and the annual cycle of the immunoreactivity in relation to the physiological changes was examined (Danton et al 1996). Recently, recombinant human insulin-like growth factor-I (IGF-I) has been shown to stimulate protein synthesis in mantle edge cells of Pacific oyster, Crassostrea gigas, and a partial cDNA sequence of insulin receptor-related receptor has been identified (Gricourt et al 2003).…”

Section: Introductionmentioning

confidence: 99%

cDNA structure of an insulin-related peptide in the Pacific oyster and seasonal changes in the gene expression

Hamano¹,

Awaji²,

Usuki³

2005

Journal of Endocrinology

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Insulin-related peptide cDNA was characterized in the Pacific oyster Crassostrea gigas. It was determined that three transcripts with differing lengths of 3 -untranslated region (3 -UTR) were expressed in the visceral ganglia. The insulin-related peptide cDNA contained a number of AUUUA motifs that were typical of adenylate/uridylaterich elements in the 3 -UTR. The deduced preprohormone was a polypeptide of 161 residues and showed a conformation typical of preprohormones of the insulin superfamily, which included conserved amino acids necessary to adopt the globular insulin structure. The expression of the three different transcripts was variable throughout the year, with the highest expression observed in March and lower expression in November and July.

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Insulin-like system and growth regulation in the Pacific oyster Crassostrea gigas: hrIGF-1 effect on protein synthesis of mantle edge cells and expression of an homologous insulin receptor-related receptor

Cited by 76 publications

References 86 publications

Increasing genomic information in bivalves through new EST collections in four species: Development of new genetic markers for environmental studies and genome evolution

Increasing genomic information in bivalves through new EST collections in four species: Development of new genetic markers for environmental studies and genome evolution

Sex-Specific Regulation of AMP-Activated Protein Kinase (AMPK) in the Pacific Oyster Crassostrea gigas1

cDNA structure of an insulin-related peptide in the Pacific oyster and seasonal changes in the gene expression

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