Insulin-like growth factors and their binding proteins in the heart in rats in experimental diabetes mellitus, growing Guerin’s carcinoma and under their combination

Frantsiyants, Elena Mikhaylovna; Бандовкина, В. А.; Kaplieva, Irina V.; Surikova, Ekaterina I.; Cheryarina, Natalia D.; Shikhlya­rova, Alla I.; Neskubina, Irina V.; Pogorelova, Yulia A.; Trepitaki, Lidia K.; Goroshinskaya, I. A.; Котиева, И. М.; Morozova, M.I.

doi:10.18137/cardiometry.2021.18.138144

Cited by 2 publications

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References 23 publications

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“…Неизученной остается зависимость содержания компонентов системы IGF от пола больных СМТ. При этом при разных видах экспериментальных опухолей выявлена половая специфика в динамике инсулиноподобных факторов роста 1 (insulin-like growth factor 1, IGF1) и инсулиноподобных факторов роста 2 (insulinlike growth factor 2, IGF2) в крови и тканях [12][13][14].…”

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Insulin-like growth factors and their transfer protein in the blood of patients with primary and recurrent soft tissue sarcomas

Goroshinskaya

Kaplieva

Sagatelyan

et al. 2022

jour

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Introduction. Soft tissue sarcomas (STS) are rare diseases but their characteristic tendency for recurrence and high mortality dictate the need for the search for prognostic biomarkers for identification of patients with the risk of recurrence. In this context, the system of insulin-like growth factors (IGF) and their insulin-like growth factor-binding proteins (IGFBP) is of interest because it was shown that disruption of the IGF system regulation leads to cancer cell proliferation and migration and chemotherapy resistance.The study objective is to investigate the levels of the IGF system components in blood of patients with primary and recurrent STS.Materials and methods. In total, 54 patients with STS, T2bN0M0, were examined: 12 men and 12 women with primary STS, 10 men and 20 women with recurrent STS, as well as healthy donors (10 men and 10 women). Mean patient age was 63.1 ± 0.9 years. In blood sampled prior to treatment, the levels of insulin-like growth factor 1 (IGF1), insulin-like growth factor 2 (IGF2) and insulin-like growth factor-binding protein 2 (IGFBP2) were measured by ELISA using Mediagnost kits (Germany). Statistical analysis was performed using Statistica 10 software and non-parametric Mann-Whitney test.Results. In primary STS, the levels of IGF1 in men with G3–4 tumors and all women decreased 14- and 20-fold, respectively, compared to healthy donors. Additionally, an insignificant decrease of IGF2 level in men and a decrease of IGF1/IGF2 ratio by the factor of 8.8 in men with G3–4 tumors and by the factor of 24.3 in women were observed. In recurrences, IGF1 level decreased by 40 % in men and by 78–85.5 % in women, while IGF2 level in men with G3–4 tumors decreased by 19 %, in women increased by 21–58 % compared to donors. In women with primary STS and recurrences of G3–4 tumors, IGFBP2 was also elevated. In men with G3–4 tumors, changes in IGFBP2 levels were less significant and had an opposite trend compared to women.Conclusion. STS recurrence is accompanied by imbalance of IGF system components in blood, especially in patients of both sexes with G3–4 tumors. Correlation between increased IGFBP2 level in STS and clinical characteristics of the disease, especially in recurrence, suggest prognostic significance of this molecule.

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Section: Introductionunclassified

Insulin-like growth factors and their transfer protein in the blood of patients with primary and recurrent soft tissue sarcomas

Goroshinskaya

Kaplieva

Sagatelyan

et al. 2022

jour

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Influence of diabetes mellitus on free radical processes in the heart in rats with Guerin’s carcinoma and characteristics of malignant lesions depending on the gender of animals

Frantsiyants¹,

Goroshinskaya²,

Kaplieva³

et al. 2022

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To study the intensity of lipid peroxidation (LPO) and the activity of the main antioxidant protection enzyme: superoxide dismutase (SOD) in heart tissues and tumors in rats of both genders with Guerin’s carcinoma (GC) and the tumor growth against the background of diabetes mellitus (DM). Materials and methods. Our research work was carried out in 80 outbread albino male and female rats, divided into 4 groups, with 10 animals of each gender in a group. The animals of two groups, namely, an intact animal group and a group of rats treated with alloxan DM (with a 5-fold increase in glucose levels) were subcutaneously transplanted with the Guerin’s carcinoma (GC) strain cells, and at the same time we used one group of the intact rats and another group of the animals with DM as the references. The content of malondialdehyde (MDA), diene conjugates (DC), and the SOD activity in the heart and the tumor tissues were determined by conventional spectrophotometric methods. Results. The most pronounced changes were found in the heart in the female rats with isolated GC and GC growing against the background of DM: a more than threefold increase in MDA, with a significant increase in DC and a multiple increase in the SOD activity as compared with the intact animals. In the GC tissue, the dependence of the severity of the increase in the MDA content on the size of the tumor was traced: the maximum increase in both parameters was observed in males with GC tumor growing against the background of DM. In the males, the volume of the subcutaneous tumor nodes was 1.8 times greater than that in the reference group and in the females with combined pathology, while in the females with combined pathology the volume of their tumors was 1.3 times less than that in the reference group, although the area of tumor lesions in them was maximum due to extensive metastasizing. Conclusion. DM has changed the specifics of oncogenesis depending on the gender of the animals. The identified gender differences in the redox status of the heart and the tumor in rats with combined pathology contribute to specifics of oncogenesis in males and females and determines their life expectancy.

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Insulin-like growth factors and their binding proteins in the heart in rats in experimental diabetes mellitus, growing Guerin’s carcinoma and under their combination

Cited by 2 publications

References 23 publications

Insulin-like growth factors and their transfer protein in the blood of patients with primary and recurrent soft tissue sarcomas

Insulin-like growth factors and their transfer protein in the blood of patients with primary and recurrent soft tissue sarcomas

Influence of diabetes mellitus on free radical processes in the heart in rats with Guerin’s carcinoma and characteristics of malignant lesions depending on the gender of animals

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