Insulin-like growth factor I stimulates growth in hypophysectomized rats

Schoenle, E.; Zapf, J.; Humbel, R; Froesch, E. R.

doi:10.1038/296252a0

Cited by 607 publications

(236 citation statements)

References 17 publications

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“…IGF-1, which is produced mainly in the liver in response to the action of growth hormone and is transferred into the serum [28], is known to stimulate the growth of bone and cartilage [27]. In particular, it might stimulate proteoglycan synthesis in the nucleus pulposus, as demonstrated by Thompson et al [33].…”

Section: Transforming Growth Factor-β1mentioning

confidence: 98%

Cytokines and growth factors in the protruded intervertebral disc of the lumbar spine

et al. 2002

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IntroductionThe pathogenetic mechanism of low back pain and nerve root damage in lumbar disc herniation is the subject of ongoing debate. In particular, given that the mechanicalischaemic hypothesis alone cannot explain the cause-effect relationship between disc degeneration and neurological impairment, a biochemical mechanism is likely to be involved. It has recently been demonstrated that the autologous nucleus pulposus can induce histological and functional changes in spinal nerve roots when applied epidurally [17]. Cells of the nucleus pulposus can produce prostaglandin E2, interleukin-1 and interleukin-6 (IL-6) [31], phospholipase A2 [26], and growth factors like fibroblast-like growth factor (FGF) [32] and insulin-like growth factor-1 (IGF-1) [18]. These factors are known to control cell metabolism and to promote inflammatory processes. Moreover, recent studies suggest a critical role for IL-6 and its receptor in the modulation of pain [8]. The mechanisms of action underlying the possible effect of these factors on spinal nerve root structure and function are, however, still unknown. In addition, the fact that nerve roots differ from peripheral nerves in their anatomical, biomechanical, and physiological properties prevents extrapolation from the extensive literature and the numerous experimental models of peripheral nerve compression to the pathophysiology of root injury [5,23,25]. The working hypothesis of the present study is that transforming growth factor-β1 (TGF-β1), IGF-1, IL-6 and IL-6-receptor (IL-6R) can be produced at the site of herniation. This hypothesis was immunohistochemically explored by analysing normal and protruded intervertebral disc tissue for these factors.Abstract Nerve root irritation induced by factors produced by the intervertebral disc may play a crucial role in the pathophysiology of sciatic pain production. In this study we used immunohistochemistry to investigate the presence of transforming growth factor-β1 (TGF-β1), insulinlike growth factor-1 (IGF-1), interleukin-6 (IL-6), IL-6-receptor (IL-6R) and fibronectin in lumbar disc bioptic specimens from 30 patients with disc herniation (protrusion type). Chondrocytes of herniated discs stained positive for TGF-β1, IGF-1, IL-6 and fibronectin. We demonstrated for the first time the presence of IL-6-R in the chondrocytes of herniated tissue. Specimens from autoptic healthy tissue were used as controls. In these sections no immunoreaction for TGF-β1, IL-6, or IL-6R was found, while they expressed IGF-1 and fibronectin, but in lower quantities than herniated discs. These results demonstrated the production of factors such as TGF-β1, IGF-1, IL-6, IL-6R and fibronectin at the site of lumbar disc herniation.

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Section: Transforming Growth Factor-β1mentioning

confidence: 98%

Cytokines and growth factors in the protruded intervertebral disc of the lumbar spine

et al. 2002

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“…Although GH may stimulate longitudinal bone growth in hypophysectomized rats (lsaksson, Jansson and Gause, 1982 ;Russel and Spencer, 1985), most of its effects on bone growth are mediated through somatomedin (IGF,) (Canalis, 1983). This is a single-chain peptide, isolated from human and bovine plasma (Honneger and Humbel, 1986) and actively stimulating the growth of hypophysectomized rats (Schoenle et al, 1982).…”

Section: Introductionmentioning

confidence: 99%

Plasma somatotropin and somatomedin C concentrations following GRF or TRH injections in newborn calves

Coxam¹,

Davicco²,

Opmeer³

et al. 1987

Reprod. Nutr. Dévelop.

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“…Recently, however, it has been reported that chicken and human IGF-I show almost complete homogeneity in the first 30 residues at the N-terminus (Dawe et al, 1988) (Drop et al, 1979(Drop et al, , 1984. In contrast to the larger, GHdependent binding protein, the 33 kDa protein found in early and mid-gestation human fetuses has been shown to potentiate, rather than inhibit, the action of IGF-I (Elgin et al, 1984); -in the absence (or large reduction) of 150 kDa binding proteins, the half-life of IGF-I in the hypophysectomized rat is less than 30 min (Zapf et al, 1986) and the effects of IGF-I on growth in hypophysectomized rats has only been observed with continuous infusion or daily injections (Schoenle et al, 1982 (Hizuka et al, 1986) Thus endogenous IGF-I production in the normal chick embryo may be sufficient to allow maximal growth.…”

Section: Methodsmentioning

confidence: 99%

“…Nous avons donc étudié l'effet de l'administration de 500 ng d'IGF pur injecté in ovo à j7 et j14 dans le sac allantoïdien. Tous les ceufs ont éclos le même jour et les poussins traités et témoins ( Brande, 1979) and hypophysectomized rats (Schoenle et al, 1982). However, there is little in the way of direct evidence that somatomedins stimulate growth of normal animals in vivo.…”

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Lack of effect of exogenous insulin-like growth factor-I (IGF-I) on chick embryo growth rate

Spencer¹,

Garssen²,

Gerrits³

et al. 1990

Reprod. Nutr. Dévelop.

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Insulin-like growth factor I stimulates growth in hypophysectomized rats

Cited by 607 publications

References 17 publications

Cytokines and growth factors in the protruded intervertebral disc of the lumbar spine

Cytokines and growth factors in the protruded intervertebral disc of the lumbar spine

Plasma somatotropin and somatomedin C concentrations following GRF or TRH injections in newborn calves

Lack of effect of exogenous insulin-like growth factor-I (IGF-I) on chick embryo growth rate

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