Insulin-Like Growth Factor-I and Insulin-Like Growth Factor Binding Protein-2 and -5 in Equine Seminal Plasma: Association with Sperm Characteristics and Fertility1

Macpherson, M. L.; Simmen, Rosalia C. M.; Simmen, Frank A.; Hernández, Jesús García; Sheerin, Barbara R.; Varner, D.D.; Loomis, P.R.; Cadario, María Estela; Miller, C D; Brinsko, Steven P.; Rigby, Sherri L.; Blanchard, T.L.

doi:10.1095/biolreprod67.2.648

Cited by 60 publications

(52 citation statements)

References 52 publications

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“…In the present study, no difference was observed between the genetic backgrounds (data not shown). In stallions, differences in IGF-I concentrations were not detected between samples from individual animals at different levels of sexual activity, but substantial variation in IGF-I concentrations occurred between stallions (Macpherson et al, 2002). In the present study, no correlation (p>0.05) was observed between the free IGF-I concentration in the seminal plasma of boars and most of the evaluated seminal parameters, except for the motility degradation rate.…”

Section: Discussioncontrasting

confidence: 75%

“…The knowledge of the number of receptors in different species may help to elucidate the true role of IGF-I in spermatozoal metabolism and the usefulness of this hormone in the improvement of artificial insemination programs. Pregnancy rates were higher for horses with high seminal plasma IGF-I levels (Macpherson et al, 2002), which is suggestive of the effects of IGF-I on sperm function.…”

Section: Discussionmentioning

confidence: 86%

“…In other studies, total IGF-I was measured. For instance, in buffaloes, Selvaraju et al (2009) (Henricks et al, 1998) in bovine seminal plasma, and in stallion from 10.2 to 10.4 ng/mL (Macpherson et al, 2002). These values show that the levels in boars and stallions are similar to those in humans (Lackey et al, 2002), but much lower than those found in cattle and buffaloes.…”

Section: Discussionmentioning

confidence: 89%

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Identificação do fator de crescimento semelhante à insulina i no plasma seminal de varrões e sua influência na qualidade espermática

et al. 2012

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A presente pesquisa foi realizada para investigar as relações entre os parâmetros dos espermatozóides e as concentrações de IGF-I no plasma seminal de varrões. Amostras de sêmen (duplicatas) foram coletadas de machos maduros (n=27). Em cada ejaculação, as características macroscópicas e microscópicas foram determinadas. Após a centrifugação para separar espermatozóides do plasma seminal, as concentrações de IGF-I em amostras de plasma seminal foram determinadas por kit de imunoensaio humano específico. A concentração média de IGF-I do plasma seminal de varrões foi de 1,5±0,2 ng/mL (n=54 amostras). Não houve correlação (p>0,05) entre IGF-I com a maioria dos parâmetros avaliados. Houve apenas uma correlação baixa negativa (-0,29, p=0,0331) entre a concentração de IGF-I no plasma seminal e a taxa de degradação da motilidade. Este estudo sugere que o IGF-I no plasma seminal de varrões não está relacionado com os parâmetros seminais iniciais do sêmen in natura. No entanto, o hormônio aumentou a duração da motilidade espermática.

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Section: Discussioncontrasting

confidence: 75%

Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 89%

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Identificação do fator de crescimento semelhante à insulina i no plasma seminal de varrões e sua influência na qualidade espermática

et al. 2012

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“…However, significant differences between both sexes were only found in foals aged 5-12 months and stallions aged 2-6 years. It is known that androgens increase the number of receptors for GH on the liver and therefore, greater release of IGF-1 to the bloodstream is expected (Clapper et al, 2000), even though it has been suggested that also the hepatic synthesis of IGF-1 is stimulated by both androgens and estrogens (McPherson et al, 2002). The period of time between two and four years of age is considered to constitute puberty in the Andalusian male and this fact could be one plausible explanation for the higher serum IGF-1 concentrations.…”

Section: Influence Of Sex On Serum Igf-1 Concentrations In Andalusianmentioning

confidence: 99%

A study of serum insulin-like growth factor type 1 (IGF-1) concentrations in resting untrained Andalusian horses: influence of age and gender

Muñoz¹,

Trigo²,

Riber³

et al. 2011

Vet. Med.

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Growth rate, tissue repair and reproductive functions are mediated by the somatotrophic axis, the growth hormone (GH) being one of its main components. GH is released in a pulsatile manner and a single measurement does not provide accurate information on the activity of the somatotrophic axis. The actions of GH on tissues are mediated by insulin-like growth factor type 1 (IGF-1), mainly released by the liver, and thus, the measurement of IGF-1 could be considered a good indicator of the activity of GH and the somatotrophic axis. Serum IGF-1 concentrations are relatively stable due to its long biological half-life without obvious diurnal rhythm. Additionally, many diseases significantly alter circulating IGF-1 concentrations, leading to potential diagnostic and prognostic uses in veterinary medicine. However, serum IGF-1 concentrations are affected by many factors, such as breed, age and sex. The present study analyzes the influence of these factors on serum IGF-1 concentrations in a population of 255 Andalusian horses (141 females and 114 males), divided into age groups: 1-2, 2-3, 3-4, 4-5, 5-6 and 6-12 months and 1-2, 2-4, 4-6, 6-10 and 10-14 years. The animals belonged to six different farms located in the same geographic location and were subjected to similar feeding and management protocols. Two measurements of body size were made: height at the withers (HW) and diameter of the thorax (DTx). Blood samples were taken always in the morning, in the month of July and serum IGF-1 concentrations were measured with a sandwich ELISA after dissociation of IGF-1 from its binding proteins. It was found that age and sex significantly influenced serum IGF-1 concentrations, whereas the effects of the farm and the time of blood withdrawal were not significant. Mean serum concentrations for both males and females respectively were: 246.3 and 231.0 (1-2 months), 201.9 and 194.7 (2-3 months), 174.2 and 170.4 (3-4 months), 161.7 and 155.4 (4-5 months), 166.1 and 136.9 (5-6 months), 127.2-114.5 (6-12 months), 103.3 and 89.01 (1-2 years), 104.3 and 73.41 (2-4 years), 105.4 and 64.40 (4-6 years), 53.29 and 68.27 (6-10 years) and 59.56 and 65.53 ng/ml (10-14 years). A progressive decrease in serum IGF-1 concentrations with increased age was found for both sexes. Males aged between five and 12 months and between two and six years had significantly higher serum IGF-1 concentrations than females of the same age. Coefficients of correlation between the indicators of body size (HW and DTx) and IGF-1 were -0.800 and -0.690 for the whole population of Andalusian horses, -0.860 and -0.750 for the males and -0.740 and -630 for the females. It is concluded that serum IGF-1 concentrations in Andalusian horses are reduced with ageing, male horses of determined age groups had higher IGF-1 than the females and there are negative correlations between body size and IGF-1 concentrations. The knowledge of the normal serum IGF-1 concentrations will help us to understand the role of the somatotrophic axis in several diseases and physiologic...

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“…It has also been shown that, immediately after the addition of this hormone in semen, sperm quality is improved in equine (Macpherson et al, 2002), buffalo (Selvaraju et al, 2009) and boar (Silva et al, 2011). Thus, a method to improve the quality of cooled boar semen could be the addition of a metabolic activator such as IGF-I, along with an antioxidant such as vitamin E.…”

Section: Introductionmentioning

confidence: 99%

Effect of the addition of IGF-I and vitamin E to stored boar semen

Mendez

Zangerônimo

Rocha

et al. 2013

Animal

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The objective of this study was to evaluate the addition of IGF-I to pig insemination doses stored at 158C, in conjunction with the addition of different amounts of vitamin E (a-tocopherol). Semen samples (n 5 12) from four boars were treated by the addition of different concentrations of vitamin E, ranging up to 400 mg/ml. Immediately after processing and after the doses had been stored at 158C for 24 or 72 h, samples were warmed at 378C and 30 ng/ml of IGF-I was added. The assessments were made after 10 and 120 min of IGF-I addition. There was a minor effect of the vitamin E added before cooling and IGF-I added after storage on sperm quality. The addition of 400 mg/ml of vitamin E to diluted semen reduced ( P , 0.01) the malondialdehyde (MDA) production in boar semen stored at 158C for 72 h, regardless of the addition of IGF-I as additive during a 120 min incubation period at 378C. In these conditions, IGF-I also reduced ( P , 0.05) the MDA production in semen samples without addition of vitamin E. IGF-I in the presence of vitamin E reduced ( P 5 0.03) the glucose intake in freshly diluted boar semen samples before cooling. It was concluded that the addition of 400 mg/ml of vitamin E reduces the MDA production in boar semen stored at 158C for 72 h, regardless of the presence of IGF-I additive. The addition of IGF-I in doses stored for 72 h with vitamin E ensures higher sperm motility after 120 min of incubation at 378C.

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Insulin-Like Growth Factor-I and Insulin-Like Growth Factor Binding Protein-2 and -5 in Equine Seminal Plasma: Association with Sperm Characteristics and Fertility1

Cited by 60 publications

References 52 publications

Identificação do fator de crescimento semelhante à insulina i no plasma seminal de varrões e sua influência na qualidade espermática

Identificação do fator de crescimento semelhante à insulina i no plasma seminal de varrões e sua influência na qualidade espermática

A study of serum insulin-like growth factor type 1 (IGF-1) concentrations in resting untrained Andalusian horses: influence of age and gender

Effect of the addition of IGF-I and vitamin E to stored boar semen

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