Insulin-Like Growth Factor Axis Expression in Dental Pulp Cells Derived From Carious Teeth

Alkharobi, Hanaa Esa; Al-Khafaji, Hasanain; Beattie, James; DeVine, Deirdre; El-Gendy, Reem

doi:10.3389/fbioe.2018.00036

Cited by 10 publications

(7 citation statements)

References 39 publications

(52 reference statements)

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“…The previous studies regarding BMSCs also showed that IGF1 promoted osteodifferentiation of BMSCs and could even be regarded as an alternative biomarker to bone morphogenetic protein-7 [ 54 , 55 ]. In addition, the IGF axis (e.g., IGF-2 and IGFBP-2) was activated under osteogenic conditions in DPSCs, indicating its promoting role in DPSC osteodifferentiation [ 56 ]. THBS1 (thrombospondin-1) is a major regulator of latent TGF- β activation [ 57 ], which can inhibit osteoblast differentiation in BMSCs.…”

Section: Discussionmentioning

confidence: 99%

Shared Genetic and Epigenetic Mechanisms between the Osteogenic Differentiation of Dental Pulp Stem Cells and Bone Marrow Stem Cells

Gaus

et al. 2021

BioMed Research International

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Objective. To identify the shared genetic and epigenetic mechanisms between the osteogenic differentiation of dental pulp stem cells (DPSC) and bone marrow stem cells (BMSC). Materials and Methods. The profiling datasets of miRNA expression in the osteogenic differentiation of mesenchymal stem cells from the dental pulp (DPSC) and bone marrow (BMSC) were searched in the Gene Expression Omnibus (GEO) database. The differential expression analysis was performed to identify differentially expressed miRNAs (DEmiRNAs) dysregulated in DPSC and BMSC osteodifferentiation. The target genes of the DEmiRNAs that were dysregulated in DPSC and BMSC osteodifferentiation were identified, followed by the identification of the signaling pathways and biological processes (BPs) of these target genes. Accordingly, the DEmiRNA-transcription factor (TFs) network and the DEmiRNAs-small molecular drug network involved in the DPSC and BMSC osteodifferentiation were constructed. Results. 16 dysregulated DEmiRNAs were found to be overlapped in the DPSC and BMSC osteodifferentiation, including 8 DEmiRNAs with a common expression pattern (8 upregulated DEmiRNAs (miR-101-3p, miR-143-3p, miR-145-3p/5p, miR-19a-3p, miR-34c-5p, miR-3607-3p, miR-378e, miR-671-3p, and miR-671-5p) and 1 downregulated DEmiRNA (miR-671-3p/5p)), as well as 8 DEmiRNAs with a different expression pattern (i.e., miR-1273g-3p, miR-146a-5p, miR-146b-5p, miR-337-3p, miR-382-3p, miR-4508, miR-4516, and miR-6087). Several signaling pathways (TNF, mTOR, Hippo, neutrophin, and pathways regulating pluripotency of stem cells), transcription factors (RUNX1, FOXA1, HIF1A, and MYC), and small molecule drugs (curcumin, docosahexaenoic acid (DHA), vitamin D3, arsenic trioxide, 5-fluorouracil (5-FU), and naringin) were identified as common regulators of both the DPSC and BMSC osteodifferentiation. Conclusion. Common genetic and epigenetic mechanisms are involved in the osteodifferentiation of DPSCs and BMSCs.

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Section: Discussionmentioning

confidence: 99%

Shared Genetic and Epigenetic Mechanisms between the Osteogenic Differentiation of Dental Pulp Stem Cells and Bone Marrow Stem Cells

Gaus

et al. 2021

BioMed Research International

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“…In 2006, Masuda et al showed that laser irradiation of rat incisors enhances GHR and IGFBP5 expression in addition to other genes related to odontoblast-differentiation and formation [ 99 ]. In 2018, Alkharobi’s group also demonstrated a novel role of IGFBP2 and IGFBP3 in cell response to mild inflammatory environment associated with superficial caries by showing that DPCs from teeth with superficial caries display higher levels of IGFBP2 and IGFBP3 when compared to cells isolated from healthy teeth [ 100 ].…”

Section: Gh/igf and Oral Tissue Engineeringmentioning

confidence: 99%

The Role of GH/IGF Axis in Dento-Alveolar Complex from Development to Aging and Therapeutics: A Narrative Review

Koffi

Doublier

Ricort

et al. 2021

Cells

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The GH/IGF axis is a major regulator of bone formation and resorption and is essential to the achievement of normal skeleton growth and homeostasis. Beyond its key role in bone physiology, the GH/IGF axis has also major pleiotropic endocrine and autocrine/paracrine effects on mineralized tissues throughout life. This article aims to review the literature on GH, IGFs, IGF binding proteins, and their respective receptors in dental tissues, both epithelium (enamel) and mesenchyme (dentin, pulp, and tooth-supporting periodontium). The present review re-examines and refines the expression of the elements of the GH/IGF axis in oral tissues and their in vivo and in vitro mechanisms of action in different mineralizing cell types of the dento-alveolar complex including ameloblasts, odontoblasts, pulp cells, cementoblasts, periodontal ligament cells, and jaw osteoblasts focusing on cell-specific activities. Together, these data emphasize the determinant role of the GH/IGF axis in physiological and pathological development, morphometry, and aging of the teeth, the periodontium, and oral bones in humans, rodents, and other vertebrates. These advancements in oral biology have elicited an enormous interest among investigators to translate the fundamental discoveries on the GH/IGF axis into innovative strategies for targeted oral tissue therapies with local treatments, associated or not with materials, for orthodontics and the repair and regeneration of the dento-alveolar complex and oral bones.

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“…Secretome analysis of cells encapsulated within NapFF ε K ε K-OH hydrogels suggested that out of five bioactive factors (VEGF, fibroblast growth factor (FGF), epidermal growth factor (EGF), platelet-derived growth factor (PDGF) and insulin-like growth factor (IGF)) commonly utilised in tissue engineering [ 52 ], all but IGF were shown to increase in NapFF ε K ε K-OH hydrogel versus 2D culture. Interestingly, within the dental pulp IGF is associated with osteo/odontogenic differentiation [ 53 , 54 ] and levels may not therefore be expected to be so closely linked with angiogenesis or vascularisation.…”

Section: Discussionmentioning

confidence: 99%

Ultrashort Peptide Hydrogels Display Antimicrobial Activity and Enhance Angiogenic Growth Factor Release by Dental Pulp Stem/Stromal Cells

et al. 2021

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Recent studies on peptide hydrogels have shown that ultrashort peptides (<8 amino acids) can self-assemble into hydrogels. Ultrashort peptides can be designed to incorporate antimicrobial motifs, such as positively charged lysine residues, so that the peptides have inherent antimicrobial characteristics. Antimicrobial hydrogels represent a step change in tissue engineering and merit further investigation, particularly in applications where microbial infection could compromise healing. Herein, we studied the biocompatibility of dental pulp stem/stromal cells (DPSCs) with an ultrashort peptide hydrogel, (naphthalene-2-ly)-acetyl-diphenylalanine-dilysine-OH (NapFFεKεK-OH), where the epsilon (ε) amino group forms part of the peptide bond rather than the standard amino grouping. We tested the antimicrobial properties of NapFFεKεK-OH in both solution and hydrogel form against Staphylococcus aureus, Enterococcus faecalis and Fusobacterium nucleatum and investigated the DPSC secretome in hydrogel culture. Our results showed NapFFεKεK-OH hydrogels were biocompatible with DPSCs. Peptides in solution form were efficacious against biofilms of S. aureus and E. faecalis, whereas hydrogels demonstrated antimicrobial activity against E. faecalis and F. nucleatum. Using an angiogenic array we showed that DPSCs encapsulated within NapFFεKεK-OH hydrogels produced an angiogenic secretome. These results suggest that NapFFεKεK-OH hydrogels have potential to serve as novel hydrogels in tissue engineering for cell-based pulp regeneration.

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Insulin-Like Growth Factor Axis Expression in Dental Pulp Cells Derived From Carious Teeth

Cited by 10 publications

References 39 publications

Shared Genetic and Epigenetic Mechanisms between the Osteogenic Differentiation of Dental Pulp Stem Cells and Bone Marrow Stem Cells

Shared Genetic and Epigenetic Mechanisms between the Osteogenic Differentiation of Dental Pulp Stem Cells and Bone Marrow Stem Cells

The Role of GH/IGF Axis in Dento-Alveolar Complex from Development to Aging and Therapeutics: A Narrative Review

Ultrashort Peptide Hydrogels Display Antimicrobial Activity and Enhance Angiogenic Growth Factor Release by Dental Pulp Stem/Stromal Cells

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