Insulin-like growth factor and insulin receptors in intestinal mucosa of neonatal calves

Georgiev, Ivan; Georgieva, T. M.; Pfaffl, Michael W.; Hammon, H.M.; Blum, J.W.

doi:10.1677/joe.0.1760121

Cited by 61 publications

(46 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One could speculate that colostral IGF-I was intestinally absorbed and that Dexa treatment might have stimulated the absorption of colostral IGF-I, thus resulting in reduced hepatic IGF-I expression in colostrum-fed calves because of feedback inhibition (20,38). However, there was no evidence for increased absorption of macromolecules like IgG in Dexa-treated calves (Sauter SN, Blum JW, and Hammon HM, unpublished observation), and we could not demonstrate significant absorption of IGF-I in neonatal calves (20,50), although receptors for IGF-I are present in intestinal mucosa (18,22). Different feeding may influence hepatic IGF-I mRNA stability after Dexa treatment and may regulate IGF-I production at the posttranscriptional level.…”

Section: Discussionmentioning

confidence: 57%

“…However, concentrations of biologically active factors like IGF-I or insulin were much lower in the three formulas than in colostrum and possibly affected neonatal development because receptors for IGF-I and insulin are present in the gut mucosa of neonatal calves (18,22). Body weight remained stable during the experimental period, and Dexa treatment or different feeding did not affect growth performance, yet the experimental period of 5 days was too short to get reliable data on effects of Dexa on growth performance, as was the case in 18-day-old pigs (10).…”

Section: Discussionmentioning

confidence: 93%

“…Total RNA was reverse transcribed into cDNA with hexamer primers (Pharmacia Biotech, Buckinghamshire, UK), as described previously (40). Materials and procedures for RT-PCR, including primers for bovine IGF-I, IGF-II, IGFBP-2, IGFBP-3, and GHR mRNA, were recently described in detail (18,40). RT-PCR quantification was performed with the LightCycler system (Roche Molecular Biochemicals, Rotkreuz, Switzerland), using the software package 3.3 (Roche Molecular Biochemicals).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Effects of dexamethasone and colostrum intake on the somatotropic axis in neonatal calves

Sauter¹,

Ontsouka²,

Roffler³

et al. 2003

American Journal of Physiology-Endocrinology and Metabolism

View full text Add to dashboard Cite

Glucocorticoids and colostrum feeding influence postnatal maturation of the somatotropic axis. We have tested the hypothesis that dexamethasone (Dexa) affects the somatotropic axis in neonatal calves dependent on colostrum intake. Calves were fed either with colostrum or with a milk-based formula ( n = 14/group), and, in each feeding group, one-half of the calves were treated with Dexa (30 μg · kg body wt-1 · day-1). Pre- and postprandial blood samples were taken on days 1, 2, 4, and 5, and liver samples were taken on day 5 of life. Dexa increased insulin-like growth factor (IGF)-I, but decreased growth hormone (GH) and IGF-binding protein (IGFBP)-1 and -2 plasma concentrations and increased GH receptor (GHR) mRNA levels in liver. Dexa increased IGF-I mRNA levels only in formula-fed calves and increased hepatic GHR binding capacity, but only in colostrum-fed calves. Colostrum feeding decreased IGFBP-1 and -2 plasma concentrations and hepatic IGFBP-2 and -3 mRNA levels. In conclusion, Dexa and colostrum feeding promoted maturation of the somatotropic axis. Dexa effects partly depended on whether colostrum was fed or not.

show abstract

Section: Discussionmentioning

confidence: 57%

Section: Discussionmentioning

confidence: 93%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Effects of dexamethasone and colostrum intake on the somatotropic axis in neonatal calves

Sauter¹,

Ontsouka²,

Roffler³

et al. 2003

American Journal of Physiology-Endocrinology and Metabolism

View full text Add to dashboard Cite

show abstract

“…Since the nutrient intakes were similar in this study, nonnutrient components in the colostrum such as immunoglobulins, hormones, enzymes, growth factors, polyamines, and cytokines [15] may have affected growth and maturation of the neonatal GIT [1,2,8,9,11]. Thus, colostral insulin and IGF-I and IGF-II may in part be responsible for the enhanced development [9,10,16,31], since their specific receptors are present in the intestinal mucosa during this period [16,32,33]. These results were supported by recent findings in neonatal calves fed with a formula as in the present study together with a whey extract derived from bovine colostrum that contained primarily bioactive substances such as IGF-I, IGF-II, insulin, or lactoferrin [34].…”

Section: Feeding Effectsmentioning

confidence: 82%

Intestinal Development in Neonatal Calves: Effects of Glucocorticoids and Dependence on Colostrum Feeding<sup>1</sup>

et al. 2004

View full text Add to dashboard Cite

The neonatal development of the gastrointestinal tract around parturition in precocious mammals is greatly affected by endocrine factors like glucocorticoids as well as by nutritional factors. We have studied the effects of glucocorticoids and colostrum supply on intestinal morphology, cell proliferation, digestive enzyme activities, and xylose absorption in neonatal calves to test the hypothesis that the intestinal development in neonatal calves is influenced by glucocorticoids, dependent on colostrum feeding. Calves designated GrFD^– and GrFD⁺ were fed a milk-based formula, whereas those designated GrCD^– and GrCD⁺ received colostrum. Dexamethasone (DEXA; 30 µg/kg/day) was injected at feeding times to calves of GrFD⁺ and GrCD⁺. On day 3, the D-xylose absorption was measured. The calves were euthanized on day 5 of life. Colostrum feeding increased villus sizes in jejunum and ileum, enhanced xylose absorption capacity, and increased peptidase activities in the ileum. DEXA treatment diminished sizes and cell proliferation rates of Peyer’s patches in the ileum, yet increased proliferation of crypt cells in the ileum of formula-fed calves. DEXA reduced aminopeptidase N activities in the jejunum of formula-fed calves, but increased the peptidase activities mainly of colostrum-fed calves in the ileum. Thus, DEXA effects depended on intestinal segment and on different feeding, resulting in stimulation of crypt cell proliferation in the less mature ileum (of formula-fed calves) and in stimulation of peptidase activities in the more mature ileum (of colostrum-fed calves). We conclude that the effects of DEXA were related to the developmental stage of the neonatal intestine and promoted the intestinal development, depending on the developmental stage.

show abstract

“…This underscores the role of IGF-II as an autocrine/paracrine-acting tissue growth factor [50] that influences growth and differentiation of the central nervous system and intestinal maturation [51, 52]. Although IGF-I is only present at low concentrations in mother’s milk, it is known to have an important impact on gastrointestinal development and is even applied in the therapy of small bowel disease in animals [21, 27,53,54,55]. …”

Section: Discussionmentioning

confidence: 99%

Insulin-Like Growth Factors and Binding Proteins in Early Milk from Mothers of Preterm and Term Infants

et al. 2007

View full text Add to dashboard Cite

Breast-fed preterm infants often show a better outcome, partly ascribed to the benefit of insulin-like growth factors (IGFs) and their binding proteins (IGFBP). We compared IGF-I, IGF-II, IGFBP-2 and IGFBP-3 levels, measured by radioimmunoassays in milk samples from 30 mothers of preterm (<31 weeks) and from 19 mothers of term (>37 weeks) infants at days 7 and 21 postpartum. Proteolysis of IGFBP-2 within mother’s milk and digestion of ¹²⁵I-IGF-II and ¹²⁵I-IGFBP-2 by gastric juice from neonates were assessed by electrophoretic techniques. Mean concentrations did not differ between preterm and term milk: IGF-I (2.8 ± 0.2 vs. 2.3 ± 0.1 ng/ml), IGF-II (12.0 ± 0.4 vs. 12.2 ± 0.5 ng/ml), IGFBP-3 (100.0 ± 5.1 vs. 80.0 ± 5.8 ng/ml), but did so for IGFBP-2 (3,144 ± 172 vs. 2,428 ± 188 ng/ml, p < 0.02). Immunoblots revealed 42% (p < 0.05) more IGFBP-2 fragments of 14 and 25 kDa in preterm milk. Incubation with gastric juice caused cleavage of ¹²⁵I-IGFBP-2 and partial cleavage of ¹²⁵I-IGF-II. Mutual complexation protected IGF-II and IGFBP-2 from cleavage, suggesting that both are likely to arrive in the bowel in an intact form to exert promotive effects. The results provide further evidence that IGFBP-2 and IGF-II in breast milk are relevant factors for the early development of preterm infants.

show abstract

Insulin-like growth factor and insulin receptors in intestinal mucosa of neonatal calves

Cited by 61 publications

References 39 publications

Effects of dexamethasone and colostrum intake on the somatotropic axis in neonatal calves

Effects of dexamethasone and colostrum intake on the somatotropic axis in neonatal calves

Intestinal Development in Neonatal Calves: Effects of Glucocorticoids and Dependence on Colostrum Feeding<sup>1</sup>

Insulin-Like Growth Factors and Binding Proteins in Early Milk from Mothers of Preterm and Term Infants

Contact Info

Product

Resources

About