Insulin-like growth factor-1 stimulates vascular smooth muscle cell proliferation in rat aorta in vivo

Chen, Y.; Capron, L; Magnusson, Jan; Wallby, Lars; Arnqvist, Hans J.

doi:10.1016/s1096-6374(98)80125-1

Cited by 26 publications

(17 citation statements)

References 24 publications

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“…In fact, IGF1 was the only parameter related to AASI at multiple linear regression analysis, and IGF1 was significantly associated with the highest AASI tertile at multivariate logistic regression analysis in the acromegalic group. In this regard, the GH/IGF1 excess is thought to promote migration and proliferation of vascular smooth muscle cells (37) and to produce in animal models an increase in collagen type III (38), resulting in derangement of vessel mechanical properties.…”

Section: Discussionmentioning

confidence: 99%

Ambulatory arterial stiffness indexes in acromegaly

Dassiè¹,

Grillo

Carretta

et al. 2012

European Journal of Endocrinology

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Objective: Acromegaly is associated with increased cardiovascular morbidity and mortality and with specific heart and vascular abnormalities. The aim of our study was to investigate arterial stiffness using the ambulatory arterial stiffness index (AASI) and symmetric AASI (Sym-AASI), two indexes derived from 24-h ambulatory blood pressure monitoring (ABPM), in a group of normotensive and hypertensive patients with active acromegaly, compared with normotensive controls (NOR-CTR) or hypertensive controls (HYP-CTR). Subjects and methods: Ninety-six consecutive patients with active acromegaly (46 males, mean age 49G14 years) underwent 24-h ABPM and evaluation of cardiovascular risk factors. Based on ABPM measurement, acromegalic patients were divided into 64 normotensive (normotensive acromegalic patients (NOR-ACRO)) and 32 hypertensive (hypertensive acromegalic patients (HYP-ACRO)) patients, and were compared with 35 normotensive (NOR-CTR) and 34 hypertensive (HYP-CTR) age-, sex,-and ABPM-matched control subjects. Results: The AASI and Sym-AASI indexes were significantly higher in acromegalic patients than in controls, either in the normotensive (NOR-ACRO vs NOR-CTR, P!0.0001 for AASI and PZ0.005 for Sym-AASI) or in the hypertensive (HYP-ACRO vs HYP-CTR, PZ0.01 for AASI and PZ0.01 for Sym-AASI) group. Multiple logistic regression analysis showed a significant association of the highest AASI tertile with serum IGF1 (PZ0.034) in the whole acromegalic group. Conclusion: AASIs are increased in acromegaly, independent of blood pressure (BP) elevation, and may have an important role in predicting cardiovascular risk in this disease.

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Section: Discussionmentioning

confidence: 99%

Ambulatory arterial stiffness indexes in acromegaly

Dassiè¹,

Grillo

Carretta

et al. 2012

European Journal of Endocrinology

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“…However, postmortem studies have shown the involvement of small vessels and the thickening of the intramural vessels in up to 22% of patients [10] . Proximal arteries are usually normal, but may be tortuous, enlarged and occasionally stenotic [11] . The GH influences the endothelium directly via endothelial IGF-1 receptors or indirectly through effects on lipid metabolism, and lipid metabolism abnormalities may occur in acromegalic patients.…”

Section: Discussionmentioning

confidence: 99%

Impairment in Flow-Mediated Vasodilatation of the Brachial Artery in Acromegaly

Baykan¹,

Erem

Gedikli

et al. 2009

Med Princ Pract

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Objective: The aim of this study was to assess flow-mediated dilatation (FMD) of the brachial artery in patients with acromegaly. Subjects and Methods: We prospectively evaluated 25 patients with acromegaly (14 females, 11 males; aged 42 ± 12 years; growth hormone (GH) levels 34 ± 14 ng/ml) and 27 control subjects (15 females, 12 males; aged 45 ± 8 years; GH levels 3 ± 1.5 ng/ml). The patients and controls were matched for age, gender, body mass index, cigarette smoking, blood pressure, lipid levels, diabetes mellitus, hypertension, and its duration. Endothelial function, measured as FMD of the brachial artery using ultrasound, was calculated in the 2 groups. The endothelial function was evaluated by assessing 1-min postischemic FMD of the brachial artery. Results: The FMD was lower in patients with acromegaly (9.97 ± 3.5%) than in controls (16.1 ± 3.4%), and the difference was statistically significant (p = 0.0001). Conclusion: Endothelial dysfunction may develop in the preclinical phase of atherosclerosis in patients with acromegaly. Endothelium-dependent FMD may be impaired in acromegalic patients, and measurement of endothelial function may identify high-risk individuals earlier.

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“…Hodik et al (24), using the same model, described the stimulation of DNA synthesis by IGF-I. IGF-I was also shown to stimulate thymidine incorporation in rat muscle (6). Similar studies have also been carried out in established cell lines, for example, mouse C2C12 cells, where it was shown that insulin can increase thymidine incorporation in a dose-dependent manner (8).…”

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confidence: 89%

Metabolic and mitogenic effects of IGF-I and insulin on muscle cells of rainbow trout

Castillo

Codina

Martínez

et al. 2004

American Journal of Physiology-Regulatory, Integrative and Comp

137

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The relative function of IGF-I and insulin on fish muscle metabolism and growth has been investigated by the isolation and culture at different stages (myoblasts at day 1, myocytes at day 4, and myotubes at day 10) of rainbow trout muscle cells. This in vitro model avoids interactions with endogenous peptides, which could interfere with the muscle response. In these cells, the effects of IGF-I and insulin on cell proliferation, 2-deoxyglucose (2-DG), and l-alanine uptake at different development stages, and the use of inhibitors were studied and quantified. Insulin (10-1,000 nM) and IGF-I (10-100 nM) stimulated 2-DG uptake in trout myocytes at day 4 in a similar manner (maximum of 124% for insulin and of 142% for IGF-I), and this stimulation increased when cells differentiated to myotubes (maximum for IGF-I of 193%). When incubating the cells with PD-98059 and especially cytochalasin B, a reduction in 2-DG uptake was observed, suggesting that glucose transport takes place through specific facilitative transporters. IGF-I (1-100 nM) stimulated the l-alanine uptake in myocytes at day 4 (maximum of 239%), reaching higher values of stimulation than insulin (100-1,000 nM) (maximum of 160%). This stimulation decreased when cells developed to myotubes at day 10 (118% for IGF-I and 114% for insulin). IGF-I (0.125-25 nM) had a significant effect on myoblast proliferation, measured by thymidine incorporation (maximum of 170%), and required the presence of 2-5% fetal serum (FBS) to promote thymidine uptake. On the other hand, insulin was totally ineffective in stimulating thymidine uptake. We conclude that IGF-I is more effective than insulin in stimulating glucose and alanine uptake in rainbow trout myosatellite cells and that the degree of stimulation changes when cells differentiate to myotubes. IGF-I stimulates cell proliferation in this model of muscle in vitro and insulin does not. These results indicate the important role of IGF-I on growth and metabolism of fish muscle.

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Insulin-like growth factor-1 stimulates vascular smooth muscle cell proliferation in rat aorta in vivo

Cited by 26 publications

References 24 publications

Ambulatory arterial stiffness indexes in acromegaly

Ambulatory arterial stiffness indexes in acromegaly

Impairment in Flow-Mediated Vasodilatation of the Brachial Artery in Acromegaly

Metabolic and mitogenic effects of IGF-I and insulin on muscle cells of rainbow trout

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