Insulin-like growth factor 1 promotes neural differentiation of human stem cells from the apical papilla

Cui, Yujia; Bai, Mingru; Guo, Daimo; Yang, Yueyi; Chen, Haoran; Sun, Jing; Xie, Jing

doi:10.1016/j.archoralbio.2021.105264

Cited by 2 publications

(7 citation statements)

References 59 publications

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“…The specific methods of isolation, primary culture, and identification were described in our previous experiment. 8 Briefly, we separated the apical papilla and minced them into small pieces in phosphate-buffered saline (PBS) solution. Then, the tissues were digested with 0.5% type I collagenase (w/v, Sigma-Aldrich, St. Louis, MO).…”

Section: Methodsmentioning

confidence: 99%

“…Human SCAPs were isolated from the immature third molar of three healthy donors (16–20 years old) with informed patient consent. The specific methods of isolation, primary culture, and identification were described in our previous experiment . Briefly, we separated the apical papilla and minced them into small pieces in phosphate-buffered saline (PBS) solution.…”

Section: Methodsmentioning

confidence: 99%

“…6,7 The multidifferentiation capacity toward osteogenesis and neurogenesis was also verified by our previous articles. 8,9 Hence, SCAPs are recognized as a superior cell source for tissue regeneration. And exploring the use of SCAPs in cartilage tissue engineering is sufficiently practicable and increasingly attractive.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, SCAPs can be easily isolated with a limited invasiveness and a greater cell viability than BMSCs . Among five different postnatal dental stem/progenitor cells, including dental pulp stem cells (DPSCs), stem cells from exfoliated deciduous teeth (SHED), periodontal ligament stem cells (PDLSCs), SCAPs, and dental follicle progenitor cells (DFPCs), SCAPs are specifically derived from a developing tissue and show distinctive advantages over other counterparts in multilineage differentiation potential under certain inducing circumstances. , The multidifferentiation capacity toward osteogenesis and neurogenesis was also verified by our previous articles. , Hence, SCAPs are recognized as a superior cell source for tissue regeneration. And exploring the use of SCAPs in cartilage tissue engineering is sufficiently practicable and increasingly attractive.…”

Section: Introductionmentioning

confidence: 99%

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Microenvironmental Stiffness Directs Chondrogenic Lineages of Stem Cells from the Human Apical Papilla via Cooperation between ROCK and Smad3 Signaling

Cai

Cui

Guo

et al. 2023

ACS Biomater. Sci. Eng.

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Cell-based cartilage tissue engineering faces a great challenge in the repair process, partly due to the special physical microenvironment. Human stem cell from apical papilla (hSCAP) shows great potential as seed cells because of its versatile differentiation capacity. However, whether hSCAP has potent chondrogenic differentiation ability in the physical microenvironment of chondroid remains unknown. In this study, we fabricated poly(dimethylsiloxane) (PDMS) substrates with different stiffnesses and investigated the chondrogenic differentiation potential of hSCAPs. First, we found that hSCAPs cultured on soft substrates spread more narrowly accompanied by cortical actin organization, a hallmark of differentiated chondrocytes. On the contrary, stiff substrates were favorable for cell spreading and stress fiber formation. More importantly, the increased chondrogenic differentiation of hSCAPs seeded on soft substrates was confirmed by characterizing increased extracellular proteoglycan aggregation through Alcian blue staining and Safranin O staining and enhanced markers toward chondrogenic differentiation including SRY-box transcription factor 9 (Sox9), type II collagen (Col2), and aggrecan in both normal α-minimum essential medium (αMEM) and specific chondrogenic medium (CM) culture conditions. Then, we investigated the mechanosensing/mechanotransduction governing the chondrogenic differentiation of hSCAPs in response to different stiffnesses and found that stiffness-sensitive integrin β1 and focal adhesion kinase (FAK) were essential for mechanical signal perception and were oriented at the start of mechanotransduction induced by matrix stiffness. We next showed that the increased nuclear accumulation of Smad3 signaling and target Sox9 facilitated the chondrogenic differentiation of hSCAPs on the soft substrates and further verified the importance of Rho-associated protein kinase (ROCK) signaling in regulating chondrogenic differentiation and its driving factors, Smad3 and Sox9. By using SIS3, the specific inhibitor of p-Smad3, and miRNA targeting Rho-associated protein kinase 1 (ROCK-1), we finally confirmed the importance of ROCK/Smad3/Sox9 axis in the chondrogenic differentiation of hSCAPs in response to substrate stiffness. These results help us to increase the understanding of how microenvironmental stiffness directs chondrogenic differentiation from the aspects of mechanosensing, mechanotransduction, and cell fate decision, which will be of great value in the application of hSCAPs in cartilage tissue engineering.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Microenvironmental Stiffness Directs Chondrogenic Lineages of Stem Cells from the Human Apical Papilla via Cooperation between ROCK and Smad3 Signaling

Cai

Cui

Guo

et al. 2023

ACS Biomater. Sci. Eng.

Self Cite

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“…Co-culturing with IGF-1 leads to morphology changes in SCAPs. In addition, IGF-1 enhances the proliferation [59], osteogenic differentiation [59], and both glial and neuronal differentiation [60] of SCAPs but weakens their odontogenic differentiation [59]. Similarly, IGF-2 promotes the osteo-/dentinogenic and neurogenic differentiation potentials of SCAPs [61].…”

Section: Insulin-like Growth Factors (Igfs)mentioning

confidence: 99%

Stem Cells from the Apical Papilla (SCAPs): Past, Present, Prospects, and Challenges

Liu,

Gao,

2023

Biomedicines

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Dental diseases occurring on young permanent teeth usually lead to the premature arrest of tooth root development. Sustained tooth root elongation is necessary to achieve the goal of long-term preservation of affected teeth. To this end, stem cell-based regenerative endodontic treatment has been regarded as one of the most promising strategies for treating young permanent teeth with pulp and periapical infections. Endogenous stem cells residing in the apical papilla, named stem cells from the apical papilla (SCAPs), have been intensively investigated due to their critical roles in pulp regeneration and root redevelopment. The present review summarizes advances in the field of SCAPs studies and discusses the challenges that need to be further addressed.

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Insulin-like growth factor 1 promotes neural differentiation of human stem cells from the apical papilla

Cited by 2 publications

References 59 publications

Microenvironmental Stiffness Directs Chondrogenic Lineages of Stem Cells from the Human Apical Papilla via Cooperation between ROCK and Smad3 Signaling

Microenvironmental Stiffness Directs Chondrogenic Lineages of Stem Cells from the Human Apical Papilla via Cooperation between ROCK and Smad3 Signaling

Stem Cells from the Apical Papilla (SCAPs): Past, Present, Prospects, and Challenges

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