Insulin downregulates angiopoietin-like protein 4 mRNA in 3T3-L1 adipocytes

Yamada, Tsutomu; Ozaki, Nobuaki; Kato, Yoshiro; Miura, Yoshitaka; Oiso, Yutaka

doi:10.1016/j.bbrc.2006.07.032

Cited by 53 publications

(43 citation statements)

References 34 publications

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“…FoxO1 ⌬ 256 absence of insulin repression on basal Angptl4 expression in H4IIE cells from our experiments. In mouse 3T3-L1 adipocytes, previous studies reported that basal Angptl4 expression was suppressed by insulin ( 24,25 ). These fi ndings suggest that insulin exerts tissue-specifi c effects on basal Angptl4 expression.…”

Section: Overexpression Of Foxo1 Mutant Lacking Transactivation Domaimentioning

confidence: 71%

“…Alternatively, insulin may suppress Angptl4 expression in mouse primary hepatocytes and rat H4IIE hepatoma cells at time point(s) that we did not examine. FoxO1 is implicated in insulin-repressed basal Angptl4 expression in 3T3-L1 cells ( 24 ). It would be interesting to test whether the Angptl4 FRE we identifi ed here associates with FoxO1 in 3T3-L1 adipocytes under basal conditions.…”

Section: Overexpression Of Foxo1 Mutant Lacking Transactivation Domaimentioning

confidence: 84%

“…In 3T3-L1 adipocytes, insulin suppresses Angptl4 gene expression ( 24,25 ). Notably, in adipocytes, glucocorticoids promote lipolysis, whereas insulin inhibits this process.…”

Section: Chromatin Immunoprecipitation Assaymentioning

confidence: 99%

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Repression of glucocorticoid-stimulated angiopoietin-like 4 gene transcription by insulin

Kuo

Chen

Yan

et al. 2014

Journal of Lipid Research

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Section: Overexpression Of Foxo1 Mutant Lacking Transactivation Domaimentioning

confidence: 71%

Section: Overexpression Of Foxo1 Mutant Lacking Transactivation Domaimentioning

confidence: 84%

See 1 more Smart Citation

Repression of glucocorticoid-stimulated angiopoietin-like 4 gene transcription by insulin

Kuo

Chen

Yan

et al. 2014

Journal of Lipid Research

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“…This outcome proves that oxygen negatively regulates ANGPTL4 mRNA expression in adipocytes. Yamada et al also demonstrated a down-regulation of ANGPTL4 induced by insulin [56], suggesting the possibility that elevated ANGPTL4 expression might be involved in hypertriglyceridemia in insulin resistant states within 3T3-L1 cells. Thus, a downregulation in ANGPTL4, as it occurs with hyperoxia, might contribute to ameliorate these metabolic disorders.…”

Section: Discussionmentioning

confidence: 97%

Effects of hyperoxia exposure on metabolic markers and gene expression in 3T3-L1 adipocytes

et al. 2012

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Adipose tissue often becomes poorly oxygenated in obese subjects. This feature may provide cellular mechanisms involving chronic inflammation processes such as the release of proinflammatory cytokines and macrophage infiltration. In this context, the purpose of the present study was to determine whether a hyperoxia exposure on mature adipocytes may influence the expression of some adipokines and involve favorable changes in specific metabolic variables. 3T3-L1 adipocytes (14 days differentiated) were treated with 95% oxygen for 24 h. Cell viability, intra and extracellular reactive oxygen especies (ROS) content, glucose uptake and lactate and glycerol concentrations were measured in the culture media. Also, mRNA levels of HIF-1, leptin, IL-6, MCP-1, PPAR-, adiponectin, and ANGPTL-4 were analyzed.Hyperoxia treatment increased intra and extracellular ROS content, reduced glucose uptake and lactate release and increased glucose release. It also led to an upregulation of the expression of IL-6, MCP-1 and PPAR-, while ANGPTL4 was downregulated in the hyperoxia group with respect to control. The present data shows that hyperoxia treatment seems to provoke an inflammatory response due to the release of ROS and the upregulation of pro-inflammatory adipokines, such as IL-6 and MCP-1. On the other hand, hyperoxia may have an indirect effect on the improvement of insulin sensitivity, due to the upregulation of PPAR- gene expression as well as a possible modulation of both glucose and lipid metabolic markers. To our knownledge, this is the first study analyzing the effect of hyperoxia in 3T3-L1 adipocytes.

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“…Beside that, a large body of evidence strongly implicates insulin as the major hormone in the regulation of angptl4 expression. Yamada et al reported that angptl4 mRNA expression in 3T3 L1 adipocytes is down-regulated by insulin [22]. Ruge et al [23] showed that acute infusion of insulin caused a highly significant decrease in levels of angptl4 mRNA in adipose tissue from young humans.…”

Section: Introductionmentioning

confidence: 99%

Nutritional regulation of adipose tissue lipoprotein lipase is blunted in insulin resistant rats

et al. 2012

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Background:Hypertriglyceridemia is a common lipid abnormality accompanying insulin resistance. This study was designed to assess the contribution of dysregulation of adipose tissue lipoprotein lipase (LPL) activity to the hypertriglyceridemia in a rat model of insulin resistance. Methodology: Hereditary hypertriglyceridemic (HHTg) rats were challenged for two weeks on a high sucrose diet and LPL activity, angptl-4 expression and FFA utilisation in vitro were determined in adipose tissue. Results: Compared to control rats (Wistar), HHTg rats exhibited hyperinsulinemia, impaired fatty acid storage in adipose tissue and elevated LPL activity both in fasting and after refeeding. The expression of angiopoietin-like protein 4 (angptl4), a fastinginduced control protein for LPL activity, was not increased in adipose tissue of fasted HHTg rats as it was in the control rats. Conclusion: We conclude that LPL remains in its active form to a higher extent in HHTg rat adipose tissue due to the low expression of angptl4 on fasting. This is a possible consequence of the hyperinsulinemia. In combination with the impaired storage of fatty acids as triglycerides in adipose tissue in HHTg rats, the inflexibility of angptl4 expression may contribute to the establishment of hypertriglyceridemia in the insulin-resistant animals.

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Insulin downregulates angiopoietin-like protein 4 mRNA in 3T3-L1 adipocytes

Cited by 53 publications

References 34 publications

Repression of glucocorticoid-stimulated angiopoietin-like 4 gene transcription by insulin

Repression of glucocorticoid-stimulated angiopoietin-like 4 gene transcription by insulin

Effects of hyperoxia exposure on metabolic markers and gene expression in 3T3-L1 adipocytes

Nutritional regulation of adipose tissue lipoprotein lipase is blunted in insulin resistant rats

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