Insulin binding and sensitivity in rat skeletal muscle: effect of starvation

Brady, Linda J.; Goodman, M. N.; Kalish, Fred; Ruderman, Neil B.

doi:10.1152/ajpendo.1981.240.2.e184

Cited by 34 publications

(42 citation statements)

References 3 publications

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“…Supporting this view, we have observed that, in heart and skeletal muscle from diabetic rats, a fall in GLUT-4 protein content coincides with a decreased effect of insulin stimulating glucose transport [4,[6][7][8]. However, our study also reveals that this is not a general finding, and, whereas the effect of insulin on glucose utilization is increased in skeletal muscle during fasting [12][13][14][15], this is concomitant with either unaltered or even decreased GLUT-4 protein levels. In this regard, it should be kept in mind that insulin action relies not only on the content of cellular GLUT-4 but also on the biological activities of the insulin receptor, as well as on several post-receptor events distal to the glucose carrier itself.…”

Section: Discussionsupporting

confidence: 72%

“…In this regard, it should be kept in mind that insulin action relies not only on the content of cellular GLUT-4 but also on the biological activities of the insulin receptor, as well as on several post-receptor events distal to the glucose carrier itself. In this regard, it should be mentioned that streptozotocininduced diabetes leads to a marked impairment of tyrosine receptor kinase activity in skeletal muscle [51], and that fasting enhances insulin binding as assessed in the incubated muscle preparation [13,14].…”

Section: Discussionmentioning

confidence: 99%

“…In fasting, there is also an impaired stimulation of glucose transport in response to insulin in adipocytes [9][10][11] and heart [4]. However, an increased sensitivity and responsiveness to insulin has been reported in perfused and incubated preparations of skeletal muscle from fasted rodents [12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

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Effect of diabetes and fasting on GLUT-4 (muscle/fat) glucose-transporter expression in insulin-sensitive tissues. Heterogeneous response in heart, red and white muscle

Camps

Castelló

Muñoz

et al. 1992

Biochemical Journal

146

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1. GLUT-4 glucose-transporter protein and mRNA levels were assessed in heart, red muscle and white muscle, as well as in brown and white adipose tissue from 7-day streptozotocin-induced diabetic and 48 h-fasted rats. 2. In agreement with previous data, white adipose tissue showed a substantial decrease in GLUT-4 mRNA and protein levels in response to both diabetes and fasting. Similarly, GLUT-4 mRNA and protein markedly decreased in brown adipose tissue in both insulinopenic conditions. 3. Under control conditions, the level of expression of GLUT-4 protein content differed substantially in heart, red and white skeletal muscle. Thus GLUT-4 protein was maximal in heart, and red muscle had a greater GLUT-4 content compared with white muscle. In spite of the large differences in GLUT-4 protein content, GLUT-4 mRNA levels were equivalent in heart and red skeletal muscle. 4. In heart, GLUT-4 mRNA decreased to a greater extent than GLUT-4 protein in response to diabetes and fasting. In contrast, red muscle showed a greater decrease in GLUT-4 protein than in mRNA in response to diabetes or fasting, and in fact no decrease in GLUT-4 mRNA content was detectable in fasting. On the other hand, preparations of white skeletal muscle showed a substantial increase in GLUT-4 mRNA under both insulinopenic conditions, and that was concomitant to either a modest decrease in GLUT-4 protein in diabetes or to no change in fasting. 5. These results indicate that (a) the effects of diabetes and fasting are almost identical and lead to changes in GLUT-4 expression that are tissue-specific, (b) white adipose tissue, brown adipose tissue and heart respond similarly to insulin deficiency by decreasing GLUT-4 mRNA to a larger extent than GLUT-4 protein, and (c) red and white skeletal muscle respond to insulinopenic conditions in a heterogeneous manner which is characterized by enhanced GLUT-4 mRNA/protein ratios.

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

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Effect of diabetes and fasting on GLUT-4 (muscle/fat) glucose-transporter expression in insulin-sensitive tissues. Heterogeneous response in heart, red and white muscle

Camps

Castelló

Muñoz

et al. 1992

Biochemical Journal

146

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“…The fact that the hindquarter preparation consists of a mixture of red and white muscle fibres (Maizels, Ruderman, Goodman & Lau, 1977) while the rat soleus is composed primarily of slow-twitch, oxidative, red muscle fibres (Ariano, Armstrong & Edgerton, 1973), may have some bearing on the difference in response of ATP to fasting in the two preparations. Also, it has recently been suggested that there may be intrinsic differences between perfused and incubated muscles and that comparison of data obtained with these two preparations may be questionable (Brady, Goodman, Kalish & Ruderman, 1981).…”

Section: Implications For Diabetes Therapymentioning

confidence: 99%

Effects of streptozotocin diabetes and fasting on intracellular sodium and adenosine triphosphate in rat soleus muscle.

Moore¹,

Munford²,

Pillsworth³

1983

The Journal of Physiology

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SUMMARY1. The hypothesis that part of the insulin transduction system consists of a co-ordinated stimulation of the Na pump and of Na-H exchange by the hormone (Moore, 1981) requires that insulin plays a physiological role in the regulation of intracellular Na+. Moreover, this model predicts that in hypoinsulinaemic states, such as diabetes and fasting, intracellular pH and intracellular ATP levels would be depressed. The present study tests the hypothesis that in hypoinsulinaemic states intracellular Na+ is increased and intracellular ATP is decreased by measuring these parameters in soleus muscles removed from both diabetic and fasted rats.2. When rats were made diabetic by injection of streptozotocin (SZ) plasma insulin significantly decreased by 24 hr and plasma glucose and triglyceride levels increased.3. Intracellular Na+ was significantly elevated by 48 hr after injection of SZ. The elevation ranged from 18 to 48 % and persisted for the duration of the experimental observation (up to 28 days).4. Intracellular ATP decreased significantly by the seventh day after SZ injection and remained depressed by about 24 % for the duration (35 days) of the observation.5. In one series, a significant negative correlation was seen between plasma insulin levels and intracellular Na+ of both SZ-diabetic animals and their controls.6. Intracellular Na+ also significantly increased when hypoinsulinaemia was induced by fasting.7. Again, intracellular ATP did not decrease until after the elevation ofintracellular Na+. After 72 hr of fasting, intracellular ATP was still decreased in spite of normal plasma glucose levels. 8. Insulin therapy of SZ diabetic rats restored intracellular ATP and plasma glucose to normal, but did not restore intracellular Na+ to normal levels.9. The results confirm two predictions of the 'insulin transduction system model (Moore, 1981). Most strongly supported is that part of the model which indicates that the Na pump is regulated by physiological levels of insulin. This is especially convincing since hypoinsulinaemia produced by a non-pharmacological procedure, fasting, was associated with an increase in intracellular Na+.

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“…In vitro, insulin responsiveness was also impaired in liver (Cech et al, 1980), but not in muscle (Brady et al, 1981 Thus, in Reddy, 1979;Herrera et al, 1981;Brady et al, 1981;Le Marchand-Brustel & Freychet, 1979). These increases correlated with the decrease in plasma insulin generally observed in fasted rats (Penicaud et al, 1985).…”

Section: Introductionmentioning

confidence: 96%

Insulin receptor binding and tyrosine kinase activity in liver and skeletal muscle from fasted rats

Balage¹,

Sornet²,

Manin³

et al. 1989

Reprod. Nutr. Dévelop.

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Insulin binding and sensitivity in rat skeletal muscle: effect of starvation

Cited by 34 publications

References 3 publications

Effect of diabetes and fasting on GLUT-4 (muscle/fat) glucose-transporter expression in insulin-sensitive tissues. Heterogeneous response in heart, red and white muscle

Effect of diabetes and fasting on GLUT-4 (muscle/fat) glucose-transporter expression in insulin-sensitive tissues. Heterogeneous response in heart, red and white muscle

Effects of streptozotocin diabetes and fasting on intracellular sodium and adenosine triphosphate in rat soleus muscle.

Insulin receptor binding and tyrosine kinase activity in liver and skeletal muscle from fasted rats

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