Insulin and the insulin-like growth factors (IGFs) in preimplantation development

Kaye, Peter L.; Bell, Katherine L.; Beebe, L.F.S.; Dunglison, Greta F.; Gardner, H. G.; Harvey, Mark B.

doi:10.1071/rd9920373

Cited by 75 publications

(30 citation statements)

References 45 publications

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“…Transcripts for the receptors of insulin have been detected at all developmental stages of preimplantation bovine embryos (Watson et al 1994). Insulin stimulates both amino acid uptake and protein synthesis (Harvey & Kaye 1988, Kaye et al 1992 in mouse preimplantation embryos. We observed significant quantitative changes in the total protein profile of the immature oocytes following follicle culture in the presence of insulin.…”

Section: Discussionmentioning

confidence: 99%

“…However, insulin does not improve bovine oocyte maturation (Betteridge et al 1989) and has no effect on the fertilisation rate or subsequent development (Stubbings et al 1990). In the mouse, protein synthesis increases in the presence of insulin at the compacted morulae stage of development (Rao et al 1990, Kaye et al 1992 when the insulin receptor appears (Harvey & Kaye 1988).…”

Section: Introductionmentioning

confidence: 99%

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Dissociation of oocyte nuclear and cytoplasmic maturation by the addition of insulin in cultured bovine antral follicles

Fouladi-Nashta

Campbell²

2006

Reproduction

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Follicles of 4-8 mm in diameter were dissected from ovaries and cultured in Waymouth culture medium in the presence or absence of insulin (5 mg/ml) at 39 8C in a humidified atmosphere of 45% O 2 , 5% CO 2 and 50% N 2 for 24 h. Following follicle culture, the oocytes were collected and examined for developmental potential, total protein profile and ultrastructural aspects. Oocytes aspirated directly from follicles of the same size were used as controls. Addition of insulin to the follicle culture medium significantly reduced expression of the low molecular weight insulin-like growth factor-binding proteins (IGFBPs) in the follicular fluid, and significantly reduced the cleavage rate of subsequently matured and fertilised oocytes (0.52 vs 0.61). However, there were no differences in the proportion of cleaved embryos which developed to the blastocyst stage (0.30 vs 0.28), nor embryo quality as assessed by total cell number (137 6 8.53 vs124.6 6 6.95). The total protein profiles of immature oocytes recovered after 24 h of follicle culture were compared by PAGE. There were marked differences between the two groups, unmatured oocytes recovered from the insulin-positive follicle group showed a protein pattern similar to that of matured oocytes. In addition, examination of ultrastructural features by transmission electron microscopy indicated that oocytes from follicles cultured in the presence of insulin undergo many of the cytoplasmic changes associated with oocyte maturation. In conclusion, follicle culture in the presence of insulin is beneficial for follicular survival and significantly reduces cleavage but has no detrimental effects on the development of cultured embryos. However, many of the cytoplasmic changes associated with oocyte maturation occur prior to the induction of nuclear maturation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Dissociation of oocyte nuclear and cytoplasmic maturation by the addition of insulin in cultured bovine antral follicles

Fouladi-Nashta

Campbell²

2006

Reproduction

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“…Several lines of evidence indicate a role for the IGFs in the preimplantation period. The anabolic and mitogenic influences of IGF-I on murine preimplantation development are well characterized (Kaye et al, 1992). Studies have indicated that IGF-I exerts a beneficial effect on the development of early pig embryos in vitro (Xia et al, 1994).…”

mentioning

confidence: 99%

Expression of the insulin-like growth factor (IGF) system in the bovine oviduct at oestrus and during early pregnancy

Pushpakumara¹,

Robinson²,

Demmers³

et al. 2002

Reproduction

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Early mammalian embryo development in vitro can be enhanced by co-culture with oviductal cells and by the addition of insulin-like growth factors (IGFs). This study examined the expression patterns of the oviductal IGF system in cattle in relation to the number of days after oestrus and the presence or absence of embryos. Oviducts were collected from: (i) 66 nulliparous heifers on day 3, day 6 or day 16 after insemination and from (ii) ten non-pregnant, lactating cows on day 0 or day 1 of the oestrous cycle. Oviducts were coiled, frozen whole and sectioned for in situ hybridization. Expression patterns of mRNAs encoding IGF-I, IGF-II, type 1 IGF receptor (IGF-1R), and the IFG binding proteins (IGFBP)-1, -3 and -5 were determined from autoradiographs. Separate measurements were made for the mucosa and muscle layers of the infundibulum, ampulla and isthmus. None of the parameters measured differed between heifers with or without the presence of an embryo. mRNAs encoding IGF-I and IGF-1R were present in the mucosa and muscle of all three oviductal regions, and the highest value of IGF-I mRNA was measured in heifers on day 3. IGF-II mRNA was expressed predominantly in the muscle wall. IGFBP-1 mRNA was not detectable, whereas mRNAs encoding IGFBP-3 and -5 were expressed in both the muscle and mucosa. IGFBP-3 expression was higher in cows on day 0 and day 1 of the oestrous cycle than in heifers on day 3, day 6 and day 16 after insemination. A peak of IGFBP-5 expression was reached on day 6. Locally or systemically produced IGFs, regulated by IGFBPs, may act directly on the embryo or indirectly via modulation of oviductal secretions and muscular activity to influence the success of early embryo development.

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“…16,17 İmp lan tas yon ön ce si ev re nin, kül tü re edi len blas to sist ler de ge li şi mi uyar ma sı ve fe tal ge li şim için önem li ol du ğu gös te ril miş tir. 18,19 IGF-I ve IGF-II, pek çok hüc re ve do ku ti pi nin bü-yü me, ço ğal ma, fark lı laş ma ve me ta bo liz ma sı üze-rin de ol duk ça et ki li bir fak tör lerdir. Fe tal ve em bri yo nik bü yü me de IGF-I anah tar ro le sa hiptir.…”

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The Effect of Sildenafil Citrate on IGF-1 and bFGF Expression Acting on Endometrial Receptivity in Controlled Ovarian Hyperstimulated Rats

Filiz¹,

Biyiksiz²,

Vural³

2010

Turkiye Klinikleri J Med Sci

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Ö ÖZ ZE ET T A Am ma aç ç: : Kon trol lü ovar yen hi pers ti mü las yon (KOH) uy gu lan mış sı çan lar da sil de na fil sit ra tın (Ss) imp lan tasyon pen ce re dö ne min de en do met ri yum re sep ti vi te sin de rol oy na yan fak tör ler den in sü lin ben ze ri bü yü me faktö rü-1 (IGF-1) ve ba zik fib rob last bü yü me fak tö rü (bFGF) eks pres yon la rı na et ki si ni araş tır mak tır. G Ge e r re eç ç v ve e Y Yö ön n t te em m l le er r: : Ça lış ma mız da, üç ay lık 220-250 gram ağır lı ğın da Wis tar al bi no di şi sı çan lar kul la nıl dı. Bun lar; Kontrol, KOH, Ss ve KOH + Ss grup la rı ol mak üze re dört gru ba ay rıl dı. Sı çan lar, grup la rı na gö re ya pı lan üç gün lük uy gu la ma dan son ra dör dün cü gün çift leş mek üze re er kek sı çan lar la bir ge ce bir ara da bı ra kıl dı. Er te si gün, va jinal sme ar da sper ma to zo a gö rü len ler, ge be li ğin sı fı rın cı gü nün de ka bul edil di. Ge be li ğin üç, dört ve be şin ci gün -le rin de bu lu nan sı çan lar sak ri fi ye edil di. Ute rus la rı çı ka rı lıp ta kip le ri ya pıl dık tan son ra im mü no his to kim ya sal yön tem le bo ya na rak ke sit ler de ğer len di ril di. B Bu ul l g gu u l la ar r: : Üçün cü gün de glan du lar epi tel de en yo ğun IGF-1 im mü -no re ak ti vi te si, KOH + Ss gru bun da, lu mi nal epi tel ile stro mal hüc re ler de, Ss ve KOH + Ss grup la rın da sap tan dı. Dör dün cü gün de lu mi nal epi tel de ki im mü no re ak ti vi te, be lir gin ola rak Ss gru bun da faz lay ken be şin ci gün de lumi nal epi tel, glan du lar epi tel ve stro mal hüc re ler de en yo ğun IGF-1 im mü no re ak ti vi te si, Ss gru bun day dı. En yoğun bFGF im mü no re ak ti vi te si, üçün cü gün de lu mi nal epi tel de ve stro mal hüc re ler de Ss gru bun da, glan du lar epi tel de Ss ve KOH grup la rın da be lir len di. Dör dün cü gün de lu mi nal epi tel de ki bFGF im mü no re ak ti vi te si, be lirgin ola rak Ss gru bun da yo ğun ken glan du lar epi tel, stro mal hüc re ler, en do tel ve mi yo met ri yum da Ss ve KOH grup la rın da be lir gin di. Be şin ci gün dey se lu mi nal ve glan du lar epi tel de bFGF im mü no re ak ti vi te si, Ss gru bun da yoğun ola rak sap tan dı. S So o n nu uç ç: : Bul gu la rı mız, sil de na fil sit ra tın, sı çan lar da imp lan tas yon pen ce re dö ne min de IGF-1 ve bFGF eks pres yon la rı nı et ki le ye rek hem imp lan tas yon hem de de si du a li zas yon da rol oy na dı ğı nı gös ter miş tir.A An na ah h t ta ar r K Ke e l li i m me e l le er r: : Ovu las yon in dük si yo nu; en do met ri yum; in sü lin ben ze ri bü yü me fak tö rü I; fib rob last bü yü me fak tö rü 2; sil de na fil; em bri yo imp lan tas yo nu A AB BS S T TR RA AC CT T O Ob b j je ec c t ti i v ve e: : To in ves ti ga te the ef fect of sil de na fil cit ra te (Sc) on ex pres si ons of in su lin li ke growth factor-1 (IGF-1) and ba sic fib rob last growth fac tor (bFGF) which are two of the fac tors ta king part in en do met ri um re cep ti vity in imp lan ta ti on win dow pe ri od in rats which con trol led ova rin hypers ti mu la ti on ...

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Insulin and the insulin-like growth factors (IGFs) in preimplantation development

Cited by 75 publications

References 45 publications

Dissociation of oocyte nuclear and cytoplasmic maturation by the addition of insulin in cultured bovine antral follicles

Dissociation of oocyte nuclear and cytoplasmic maturation by the addition of insulin in cultured bovine antral follicles

Expression of the insulin-like growth factor (IGF) system in the bovine oviduct at oestrus and during early pregnancy

The Effect of Sildenafil Citrate on IGF-1 and bFGF Expression Acting on Endometrial Receptivity in Controlled Ovarian Hyperstimulated Rats

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