Insulin- and Leptin-Mediated Control of Aquaglyceroporins in Human Adipocytes and Hepatocytes Is Mediated via the PI3K/Akt/mTOR Signaling Cascade

Rodríguez, Amaia; Catalán, Victoria; Gómez-Ambrosi, Javier; García‐Navarro, Socorro; Rotellar, Fernando; Valentí, Víctor; Silva, Camilo; Gil, Marı́a J.; Salvador, Javier; Burrell, Marı́a A.; Calamita, Giuseppe; Malagón, Marı́a M.; Frühbeck, Gema

doi:10.1210/jc.2010-1408

Cited by 194 publications

(236 citation statements)

References 54 publications

(52 reference statements)

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“…Neither Aqp3 nor Aqp9 mRNA are detected by northern blotting of white or brown adipose tissue from mice (Kishida et al 2000, Maeda et al 2004, and although not supported by all studies (Rodriguez et al 2011), similar results have been obtained for human WAT (Miranda et al 2010, Lebeck et al 2012a. A recent study suggests the presence of AQP10 in human WAT with localization only to the adipocytes (Laforenza et al 2005).…”

Section: Is Aqp7 the Sole Aquaglyceroporin In Adipose Tissue?mentioning

confidence: 56%

“…Furthermore, in transgenic mice with eGFP expression driven by the AQP7 promoter, the expression of eGFP was also only found in the endothelial cells of the small capillaries within the adipose tissue (Lebeck et al 2012a). The localization of AQP7 to the endothelial cells within human adipose tissue has been confirmed by others (Rodriguez et al 2011, Laforenza et al 2013. However, in their studies, they also detected labeling for AQP7 in the adipocytes.…”

Section: Localization Of Aqp7 In Adipose Tissuementioning

confidence: 81%

“…Immunostaining for AQP7 in adipocyte cell culture models demonstrates a predominant localization in intracellular regions (Kishida et al 2000, Miranda et al 2010, Rodriguez et al 2011, which does not apply to a role in transporting glycerol across the plasma membrane. Stimulation of the cells with epinephrine/isoproterenol have been reported to result in translocation of some of the AQP7 labeling to the plasma membrane domain (Kishida et al 2000, Rodriguez et al 2011, Laforenza et al 2013, in turn suggesting that AQP7 traffics to the plasma membrane in response to lipolytic stimuli.…”

Section: Localization Of Aqp7 In Adipose Tissuementioning

confidence: 89%

“…Several studies have found a reduced AQP7 mRNA expression in SAT from obese men and women (Marrades et al 2006, CeperueloMallafre et al 2007, Rodriguez et al 2011. However, one study reported unchanged AQP7 mRNA abundance in SAT, whereas in VAT increased AQP7 gene expression was observed when comparing obese with overweight individuals from a mixed gender cohort (Miranda et al 2010).…”

Section: Figurementioning

confidence: 97%

“…However, one study reported unchanged AQP7 mRNA abundance in SAT, whereas in VAT increased AQP7 gene expression was observed when comparing obese with overweight individuals from a mixed gender cohort (Miranda et al 2010). Most of the studies evaluating the AQP7 mRNA expression in association with T2D did not find significant changes in the expression in SAT or VAT (Ceperuelo-Mallafre et al , Catalan et al 2008, Miranda et al 2009, Lebeck et al 2012a, but an increased AQP7 abundance in VAT (Miranda et al 2010, Rodriguez et al 2011 has also been reported. These studies were performed in mixed gender cohorts, except for two studies (Catalan et al 2008, Lebeck et al 2012a.…”

Section: Figurementioning

confidence: 99%

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Metabolic impact of the glycerol channels AQP7 and AQP9 in adipose tissue and liver

Lebeck

2014

Journal of Molecular Endocrinology

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Obesity and secondary development of type 2 diabetes (T2D) are major health care problems throughout the developed world. Accumulating evidence suggest that glycerol metabolism contributes to the pathophysiology of obesity and T2D. Glycerol is a small molecule that serves as an important intermediate between carbohydrate and lipid metabolism. It is stored primarily in adipose tissue as the backbone of triglyceride (TG) and during states of metabolic stress, such as fasting and diabetes, it is released for metabolism in other tissues. In the liver, glycerol serves as a gluconeogenic precursor and it is used for the esterification of free fatty acid into TGs. Aquaporin 7 (AQP7) in adipose tissue and AQP9 in the liver are transmembrane proteins that belong to the subset of AQPs called aquaglyceroporins. AQP7 facilitates the efflux of glycerol from adipose tissue and AQP7 deficiency has been linked to TG accumulation in adipose tissue and adult onset obesity. On the other hand, AQP9 expressed in liver facilitates the hepatic uptake of glycerol and thereby the availability of glycerol for de novo synthesis of glucose and TG that both are involved in the pathophysiology of diabetes. The aim of this review was to summarize the current knowledge on the role of the two glycerol channels in controlling glycerol metabolism in adipose tissue and liver. Glycerol metabolismThe metabolic switching between feeding and fasting is central to everyday life and involves tight hormonal control with effects on muscle, adipose tissue, and liver that maintains an adequate handling of metabolic precursors to support energy demands. One of the molecules affected by metabolic switching is glycerol, which is a small 3-carbon alcohol that in the fed state is stored as the backbone of triglyceride (TG) mainly in adipose tissue.In the fed state, after ingestion of dietary fats (w90% TG), !30% of TG is fully hydrolyzed into free fatty acids (FFA) and glycerol. Glycerol rapidly enters the enterocytes of the small intestine and thereafter the circulation through the portal vein and is primarily metabolized by the liver (Lin 1977). In adipose tissue, the activity of glycerol kinase (GlyK), that catalyzes the initial phosphorylation of glycerol into glycerol-3-phosphate (G3P), is negligible and therefore glycerol is not normally utilized in adipose tissue. Instead G3P used for the synthesis of TG in the fed state is derived from glycolysis.During states of increased energy demand, such as fasting and exercise, adipocyte lipolysis is increased by activation of adipose TG lipase and hormone-sensitive

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Section: Is Aqp7 the Sole Aquaglyceroporin In Adipose Tissue?mentioning

confidence: 56%

Section: Localization Of Aqp7 In Adipose Tissuementioning

confidence: 81%

Section: Localization Of Aqp7 In Adipose Tissuementioning

confidence: 89%

Section: Figurementioning

confidence: 97%

Section: Figurementioning

confidence: 99%

See 3 more Smart Citations

Metabolic impact of the glycerol channels AQP7 and AQP9 in adipose tissue and liver

Lebeck

2014

Journal of Molecular Endocrinology

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Leptin and Cognitive Function

Harvey

2013

Metabolic Syndrome and Neurological Disorders

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Aquaporin‐5 is expressed in adipocytes with implications in adipose differentiation

et al. 2015

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Aquaporins (AQPs) are membrane channels widely distributed in nature. Typically, multiple isoforms are expressed in a single tissue. The adipose tissue is no exception where several AQP members have been identified. The importance of overlapped AQPs expression is unclear, yet interisoforms interactions might be required for key cellular functions. Recently, AQP5 was described as a regulator of other AQP isoforms. Therefore, we hypothesized for a role of AQP5 in adipocyte biology. Gene expression analysis revealed the presence of AQP5 in both 3T3-L1 fibroblasts and adipocytes, being more abundant in the later. AQP5 depletion impaired adipocyte differentiation, which was confirmed by decreased expression of specific differentiation markers. By overexpressing the human AQP5 in mature adipocytes it was possible to ascertain its role as a water channel in a gain-of-function scenario. To our knowledge, this is the first time that AQP5 is reported on adipose tissue. Our data revealed AQP5 as a new player in adipose tissue biology. V C 2015 IUBMB Life, 67(1): [54][55][56][57][58][59][60] 2015

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Insulin- and Leptin-Mediated Control of Aquaglyceroporins in Human Adipocytes and Hepatocytes Is Mediated via the PI3K/Akt/mTOR Signaling Cascade

Cited by 194 publications

References 54 publications

Metabolic impact of the glycerol channels AQP7 and AQP9 in adipose tissue and liver

Metabolic impact of the glycerol channels AQP7 and AQP9 in adipose tissue and liver

Leptin and Cognitive Function

Aquaporin‐5 is expressed in adipocytes with implications in adipose differentiation

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