Insulin and Insulin–Tripalmitin Particles Produced by a Supercritical Assisted Drying Process

Hu, Xiaohui; Jiang, Maoxing; Wang, JingXian; Zhu, Lei; Zheng, Shengxuan; Snavely, William K.; Hong, Yanzheng; Su, Yongxuan; Wang, Hongtao; Li, Jun

doi:10.1080/07373937.2013.829852

Cited by 6 publications

(2 citation statements)

References 32 publications

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“…[63] Supercritical assisted drying (SAD) and a Rapid expansion of supercritical solutions (RESS)-(SAD) combined process was employed to produce fine composite particles of tripalmitin and insulin (to control thermodynamic activity of protein). [67] The SEM images of Insulin particles obtained at different pressures are shown in Figure 9.…”

Section: Supercritical Fluid Drying (Scfd)mentioning

confidence: 99%

Drying of Vaccines and Biomolecules

2020

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Vaccines are the most important life-saving prophylactic medicines administered in maintaining global health. In a pandemic situation, the manufacturing, formulation followed by preservation of vaccine are challenging areas of concern looking at the dire need of administering it to several billion people on the planet in a pandemic like situation. An important area of major concern is the potential loss of vaccine activity during storage and transportation. Scientists have developed several innovative ideas to store vaccines for a longer period of time. This article focuses on a critical overview of various methods of vaccine storage in dry powder form. Conventional vaccine drying processes, such as freeze drying under different conditions, spray drying along with the upcoming novel technologies such as spray freeze drying are described in detail for the application in vaccine drying. Some other emerging methods, such as, microwave freeze drying and atmospheric freeze drying techniques are also discussed; these could be the game changers in the future. Several drying techniques make use of protective agents during the process of drying and storage. This review also highlights a comparative study of various technological challenges in drying of vaccines in support of the current global health scenario affected by COVID19.

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Section: Supercritical Fluid Drying (Scfd)mentioning

confidence: 99%

Drying of Vaccines and Biomolecules

2020

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“…In the search for new forms of INS administration, it was reported that oral administration of this hormone has low bioavailability, due mainly to gastric pH and the enzymatic barriers of the intestinal epithelium. [21] Novel processes were employed to produce INS sub-microparticles from an ethanol/water solution [22] or solvent evaporation. Such processes were designed for diminishing the chances of protein denaturation and to be used in addition to spray drying technique for micro-and nanospheres/particles preparation.…”

Section: Introductionmentioning

confidence: 99%

High molecular weight chitosan based particles for insulin encapsulation obtained via nanospray technology

et al. 2020

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The objective of this work was to obtain Chitosan (CS) based particles for Insulin (INS) encapsulation, via nanospray drying of a feeding solution containing equal amounts of both components (0.1% w/v total solids content). The process was performed at pH 3 which is out of the range for electrostatic interactions to occur. The analysis involved the nanoparticles (NP) characterization in the solution before drying (pH 3) by dynamic light scattering (DLS) and after re-hydration at different pHs (3< pH < 11). The dried product was characterized by Fourier-transform spectroscopy (FTIR), scanning electron microscopy (SEM) and differential scanning calorimetry (DSC). FTIR allowed detecting the chemical groups involved in INS-CS interactions. The encapsulation efficiency of the glassy NP was 62.3 ± 0.32% as determined by HPLC. Upon powder re-hydration, NP of diameter <200 nm were obtained, with a minority of them exceeding the micron. The change in the shape and temperature of the main endothermic DSC peak and the higher T g value of the NP would confirm the increase in INS thermal stability when entrapped in a CS matrix. In terms of biological activity an in-vitro system was assayed. 3T3-L1 fibroblasts were exposed to INS and Insulin-Chitosan nanoparticles (INS-CS NP). Both treatments showed AKT phosphorylation, which is an indication of AKT activation. The activity of AKT plays an essential role in cell metabolism (lipid and glucose), growth, proliferation, polarity, among others. This activity is a measure of the upstream cell signals, i.e. INS's receptor activity. Phosphorylated AKT was detected during the assay time for INS-CS NP, showing remarkable differences respect to single INS. Nanodrying technology could be used to trap INS into CS matrix keeping the specific hormone functions and protecting it from the hostile conditions of the body.

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