Insulin and gender: An insulin-like gene expressed exclusively in the androgenic gland of the male crayfish

Manor, Rivka; Weil, Shalva; Oren, Shmuel; Glazer, Lilah; Aflalo, Eliahu D.; Ventura, Tomer; Chalifa‐Caspi, Vered; Lapidot, Miri; Sagi, Amir

doi:10.1016/j.ygcen.2006.09.006

Cited by 153 publications

(130 citation statements)

References 62 publications

Supporting

Mentioning

126

Contrasting

Order By: Relevance

“…The deduced amino acid sequence of the EsIAG precursor was comprised of 151 amino acids, with a 19 amino acid message peptide, 31 amino acid B chain, 62 amino acid C peptide, and a 42 amino acid A chain in a row from the N terminal to the C terminal. This organization is the same as that reported for other IAGs (Manor et al, 2007;Chung et al, 2011;Li et al, 2012b;Ma et al, 2013). Similar to IAGs reported in other crustacean species, EsIAG also had two typical R-X-X-R proteolytic cleavage motifs at the C-terminal end of the C peptide (RFRR) and at the C-terminal end of the B chain (RRTR).…”

Section: Discussionsupporting

confidence: 84%

“…Consequently, we failed to clone the E. sinensis IAG gene using degenerate primers designed based on known IAG sequences. A similar negative result was reported when attempting to identify the IAG gene from C. quadricarinatus with degenerate primers designed based on isopod sequences (Manor et al, 2007).…”

Section: Discussionsupporting

confidence: 59%

“…This AGH was characterized, and the cDNA was cloned, this is the first example in crustaceans (Okuno et al, 1999). The structure of AGH is relatively similar to that of insulin or the so-called insulin-like androgenic gland factor (IAG) (Manor et al, 2007). Since then, more than 20 different crustacean IAG genes have been cloned, such as: Callinectes sapidus, Fenneropenaeus chinensis, Macrobrachium Nipponese, Sagmariasus verreauxi and Jasus edwardsii, etc (Manor et al, 2007;Chung et al, 2011;Li et al, 2012b;Ma et al, 2013;Ventura et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

“…The structure of AGH is relatively similar to that of insulin or the so-called insulin-like androgenic gland factor (IAG) (Manor et al, 2007). Since then, more than 20 different crustacean IAG genes have been cloned, such as: Callinectes sapidus, Fenneropenaeus chinensis, Macrobrachium Nipponese, Sagmariasus verreauxi and Jasus edwardsii, etc (Manor et al, 2007;Chung et al, 2011;Li et al, 2012b;Ma et al, 2013;Ventura et al, 2014). Members of the insulinlike growth factor binding proteins (IGFBPs) can bind insulin/insulin-like growth factor, so it was speculated that an interaction between IAG and insulin-like growth factor binding protein (IGFBP) product had occurred (Manor et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Since then, more than 20 different crustacean IAG genes have been cloned, such as: Callinectes sapidus, Fenneropenaeus chinensis, Macrobrachium Nipponese, Sagmariasus verreauxi and Jasus edwardsii, etc (Manor et al, 2007;Chung et al, 2011;Li et al, 2012b;Ma et al, 2013;Ventura et al, 2014). Members of the insulinlike growth factor binding proteins (IGFBPs) can bind insulin/insulin-like growth factor, so it was speculated that an interaction between IAG and insulin-like growth factor binding protein (IGFBP) product had occurred (Manor et al, 2007). In 2013, researchers discovered that IAG gene interact with IGFBP gene products in the crayfish, Cherax quadricarinatus, which provided new clues to study the function of the crustacean AG pathway (Rosen et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Untitled

Song¹,

Xu²,

Zang³

et al. 2018

Turk. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

Androgenic gland hormone has an insulin-like structure and it is the male-specific hormone found in crustaceans. Androgenic gland hormone controls male sexual differentiation and maintains male sexual characteristics. In this study, the full-length cDNA sequence of the androgenic gland hormone gene in Eriocheir sinensis (EsIAG) was cloned. The total length of the EsIAG cDNA was 1,495 bp, encoding 151 amino acids. EsIAG has the typical conserved structure of other reported IAGs but had low sequence similarity with other crustacean IAGs. EsIAG was expressed specifically in the androgenic gland. Insulin-like growth factor binding protein 7 from the Chinese mitten crab E. sinensis (EsIGFBP7) was highly expressed in the male sex-related organs among nine crab tissues. Interactions between EsIAG and EsIGFBP7 were verified using a yeast-two hybrid assay. These results obtained in this study provide basic information about the regulatory mechanism of male sex differentiation in E. sinensis.

show abstract

Section: Discussionsupporting

confidence: 84%

Section: Discussionsupporting

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Untitled

Song¹,

Xu²,

Zang³

et al. 2018

Turk. J. Fish. Aquat. Sci.

View full text Add to dashboard Cite

show abstract

Sex Control in Cultured Decapod Crustaceans

Levy

Aflalo

Sagi

2018

Sex Control in Aquaculture

View full text Add to dashboard Cite

Effects of tributyltin exposure in hermit crabs: Clibanarius vittatus as a model

Sant’Anna¹,

Santos²,

Marchi³

et al. 2012

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Tributyltin (TBT) contamination affects the reproductive system of many species of invertebrates worldwide. The present study was designed to evaluate the effects of exposure to TBT pollution on the reproduction of the hermit crab Clibanarius vittatus. An orthogonal experiment was designed with two treatments: contamination (with or without TBT in the food) and crab sex (males and females). The animals were reared in the laboratory for nine months, and macroscopic and histological analyses of reproductive organs were carried out after the end of the experiment. Tributyltin was recorded in exposed crabs, but no morphological alterations were detected in the gonads of males, regardless of whether they were exposed to TBT. In contrast, females exposed to TBT displayed disorganization and atrophy of their ovaries, thus directly affecting reproduction in this hermit crab species. This effect observed in female hermit crabs may harm populations located in harbor regions, where TBT concentration is high, even after the worldwide TBT ban.

show abstract

Insulin and gender: An insulin-like gene expressed exclusively in the androgenic gland of the male crayfish

Cited by 153 publications

References 62 publications

Untitled

Untitled

Sex Control in Cultured Decapod Crustaceans

Effects of tributyltin exposure in hermit crabs: Clibanarius vittatus as a model

Contact Info

Product

Resources

About