Insulin ameliorates hypoxia-induced autophagy, endoplasmic reticular stress and apoptosis of myocardial cells: In vitro and ex vivo models

Liu, Tsun Jui; Yeh, Yu-Min; Lee, Wen Lieng; Wang, Li Chuan; Lee, Hsiao Wei; Shiu, Miau Tsz; Su, Chieh Shou; Lai, Hui Chin

doi:10.1016/j.ejphar.2020.173125

Cited by 11 publications

(7 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hypoxia reduced the cardiomyocyte viability, increased the autophagy and apoptosis and endoplasmic reticular (ER) stress pathway-associated apoptotic responses accompanied by an increase of proapoptotic transcriptional factor, and apoptosis in myocardial cells. In this study, the insulin signaling pathway might have a vital role in chicken cardiac response to hypoxia, which was similar to the report by Liu et al who found that insulin could effectively relieve autophagy and ER stress and prevent hypoxia-induced cellular apoptosis via PI3K/Akt signaling pathway (31). An unusual HR, blood glucose, and mean arterial BP induced by hypoxia could be restored to the normoxia state by an acute insulin supplement (32).…”

Section: Signaling Pathways Linked To the Response Of Chicken Heart T...supporting

confidence: 88%

Effects of chronic hypoxia on the gene expression profile in the embryonic heart in three Chinese indigenous chicken breeds (Gallus gallus)

Abdel-Moneim²,

Hu³

et al. 2022

Front. Vet. Sci.

View full text Add to dashboard Cite

Hypoxia exposure (HE) has adverse impacts on the embryonic development of chicken, whereas the mechanism underlying the response of the heart to HE during embryo development in birds is still unclear. Therefore, our study was designed to reveal the hub genes and the signaling pathways linked to chronic hypoxia stress. Thus, the gene expression microarray GSE12675, downloaded from the GEO database, included 12 embryonic heart samples in hypoxia and normoxia of three Chinese indigenous chicken breeds [Shouguang (SG), Tibetan (TB), and Dwarf Recessive White (DRW) chickens]. A total of 653 to 714 breed-specific differentially expressed genes (DEGs) were detected in each pairwise comparison. Gene ontology (GO) showed that the DEGs were mainly involved in biological processes, including vasoconstriction, cell differentiation, and the positive regulation of vasoconstriction. KEGG enrichment revealed that the DEGs were mainly enriched in MAPK, PPAR, insulin, adrenergic signaling in cardiomyocytes, etc. Moreover, 48 genes (e.g., SGCD, DHRS9, HELQ, MCMDC2, and ESCO2) might contribute to the response of the heart to HE. Taken together, the current study provides important clues for understanding the molecular mechanism of the heart's response to HE during the embryonic period of chicken.

show abstract

Section: Signaling Pathways Linked To the Response Of Chicken Heart T...supporting

confidence: 88%

Effects of chronic hypoxia on the gene expression profile in the embryonic heart in three Chinese indigenous chicken breeds (Gallus gallus)

Abdel-Moneim²,

Hu³

et al. 2022

Front. Vet. Sci.

View full text Add to dashboard Cite

show abstract

“…Similarly, we found 20 genes with probes hypermethylated with age and with REST motif in the vicinity in all six cell types (data not shown). Four of these (right side of Figure 5C, genes under green heading) are known to be downregulated in hypoxia (47)(48)(49)(50) Novel and important conclusions arise from our observations. First, only few CpG sites are hypomethylated and hypermethylated with aging across all circulating cells while the majority of significant age-associated methylation changes are cell-selective.…”

Section: Functional Annotation Of Age-associated Probessupporting

confidence: 55%

“…Similarly, we found 20 genes with probes hypermethylated with age and with REST motif in the vicinity in all six cell types (data not shown). Four of these (right side of Figure 5C, genes under green heading) are known to be downregulated in hypoxia (47)(48)(49)(50). These results strongly suggest a link between age-associated DNA methylation and oxygen sensing through putative regulation by transcription factors like ARNT and REST in the various immune cells.…”

Section: Functional Annotation Of Age-associated Probesmentioning

confidence: 73%

Epigenetic signature of human immune aging: the GESTALT study

Roy

Kuo

Candia

et al. 2023

Preprint

View full text Add to dashboard Cite

Age-associated DNA methylation in blood cells convey information on health status. However, the mechanisms that drive these changes in circulating cells and their relationships to gene regulation are unknown. We identified age-associated DNA methylation sites in six purified blood borne immune cell types (naïve B, naïve CD4+ and CD8+ T cells, granulocytes, monocytes and NK cells) collected from healthy individuals interspersed over a wide age range. Of the thousand of age-associated sites, only 350 sites were differentially methylated in the same direction in all cell types and validated in an independent longitudinal cohort. Genes close to age-associated hypomethylated sites were enriched for collagen biosynthesis and complement cascade pathways, while genes close to hypermethylated sites mapped to neuronal pathways. In-silico analyses showed that in most cell types, the age-associated hypo- and hypermethylated sites were enriched for ARNT (HIF1β) and REST transcription factor motifs respectively, which are both master regulators of hypoxia response. To conclude, despite spatial heterogeneity, there is a commonality in the putative regulatory role with respect to transcription factor motifs and histone modifications at and around these sites. These features suggest that DNA methylation changes in healthy aging may be adaptive responses to fluctuations of oxygen availability.

show abstract

“…HF patients have obvious cardiomyocyte apoptosis, accompanied by scarce microvessels, smooth muscle remodeling of small blood vessels and vascular cell apoptosis. How to terminate or reverse the process of cell apoptosis upon HF has become the key to the improvement of prognosis [ 14 ].…”

Section: Resultsmentioning

confidence: 99%

Protective role of emodin in rats with post-myocardial infarction heart failure and influence on extracellular signal-regulated kinase pathway

Liu¹,

Liang²

2021

Bioengineered

View full text Add to dashboard Cite

We aimed to explore the effects of emodin on the energy metabolism of myocardial cells in rats with post-myocardial infarction (MI) heart failure (HF) and the extracellular signal-regulated kinase (ERK) pathway. The model of MI was established by ligation of the left anterior descending branch. After 4 weeks, the rats with left ventricular ejection fraction (LVEF) of ≤45% were used aspost-MI HF model animals and randomly divided into model, low-dose, middle-dose, high-dose and control groups (n=10). Low-, middle- and high-dose groups were gavaged with 20 mg/kg, 40 mg/kg and 60 mg/kg emodin daily, respectively. After administration for 14 d, the changes in LVEF, left ventricular end-systolic diameter (LVESD), left ventricular end-diastolic diameter (LVEDD) and interventricular septum thickness (IVS) were analyzed. The apoptosis rate of myocardial cells was detected by TUNEL staining. The levels of serum cardiac troponin I (cTnI) and peroxisome proliferator-activated receptor-γ coactivator-1 (PGC-1) were determined using ELISA, and the expressions of mitochondrial respiratory chain complex I protein and phosphorylated-ERK (p-ERK) in myocardial tissues were determined by Western blotting. Compared with model group, LVEDD, LVESD, apoptosis rate of myocardial cells, levels of serum cTnI and PGC-1, and expressions of complex I and p-ERK in myocardial tissues significantly decreased, while LVEF and IVS increased in low-dose, middle-dose, high-dose and control groups (P<0.05). The changes in the above indices were significantly dependent on the dose of emodin (P<0.05).Emodin can significantly relieve post-MI HF, reduce the apoptosis rate of myocardial tissues, and ameliorate the cardiac function of rats.

show abstract

Insulin ameliorates hypoxia-induced autophagy, endoplasmic reticular stress and apoptosis of myocardial cells: In vitro and ex vivo models

Cited by 11 publications

References 49 publications

Effects of chronic hypoxia on the gene expression profile in the embryonic heart in three Chinese indigenous chicken breeds (Gallus gallus)

Effects of chronic hypoxia on the gene expression profile in the embryonic heart in three Chinese indigenous chicken breeds (Gallus gallus)

Epigenetic signature of human immune aging: the GESTALT study

Protective role of emodin in rats with post-myocardial infarction heart failure and influence on extracellular signal-regulated kinase pathway

Contact Info

Product

Resources

About