Insulin Accelerates Seedling Growth of Canavalia ensiformis (Jack bean)

Oliveira, Antônia Elenir Amâncio; Ribeiro, Elane da Silva; Cunha, Maura Da; Gomes, Valdirene Moreira; Fernandes, Kátia Valevski Sales; Xavier‐Filho, J.

doi:10.1023/b:grow.0000038259.34384.0c

Cited by 16 publications

(11 citation statements)

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“…The existence of insulin-like factors has been reported in spinach (Collier et al, 1987) and bean (Oliveira et al, 2004). In maize, a 5.7 kDa peptide with a structure similar to that reported for insulin and IGF-I has been purified, characterized and named insulinlike growth factor of maize (ZmIGF) (Rodríguez-Ló pez et al, 2011).…”

Section: Introductionmentioning

confidence: 89%

“…In maize, a 5.7 kDa peptide with a structure similar to that reported for insulin and IGF-I has been purified, characterized and named insulin-like growth factor of maize (ZmIGF) (Rodríguez-López et al , 2011). Even though insulin has not been found in plants, it has been reported that this hormone and IGFs induce similar responses in plants; both effectors induce germination and growth of maize seeds (Sánchez de Jiménez et al , 1999; García-Flores et al , 2001) and accelerate growth of sunflower (Goodman and Davis, 1993) and Canavalia ensiformis seedlings (Oliveira et al , 2004); and in maize both ZmIGF and insulin enhance, by TOR pathway activation, the specific mobilization to polysomes and selective translation of mRNA related to protein synthesis, like S6rp, the translation initiation factor eIFiso4E (Dinkova et al , 2007) and ribosomal proteins (as has been demonstrated recently by microarray analysis; Jiménez-López et al , 2011). Also, it has been reported recently that ZmIGF/insulin induce mitogenic activation by increasing DNA synthesis in maize tissue cultures, as well as the mobilization into polysomes of mRNA coding for the proteins associated with the cell cycle, like PCNA and cyclin D1 (Sotelo et al , 2010) which participate in the G1–S transition, as mentioned earlier.…”

Section: Introductionmentioning

confidence: 99%

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Effect of insulin on the cell cycle of germinating maize seeds (Zea maysL.)

et al. 2013

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During seed germination, metabolism is reactivated, DNA is repaired and cell division is restarted in the meristems. The mechanisms that co-ordinate cell growth and division in maize embryonic axes during germination are not well understood. However, the presence of a factor similar to IGF (insulin-like growth factor) that accelerates germination has been reported. In the present work, the regulation of the cell-cycle restart by bovine insulin [which has been demonstrated to produce similar effects as insulin-like growth factor of maize (ZmIGF) in maize seeds] was studied in germinating embryonic axes. Our results showed that bovine insulin differentially stimulates growth, S6K phosphorylation, S6rp transcript accumulation on the polysomal fraction, as well as de novo DNA synthesis in the radicles and the coleoptiles of the embryonic axis. A stronger and earlier effect was observed in radicles compared to coleoptiles; therefore, the effect of insulin on the cell cycle of the root meristem was studied by flow cytometry. The G1–S transition was stimulated and cell proliferation was induced. Furthermore, it was determined by quantitative reverse transcription-polymerase chain reaction (qRT-PCR) that bovine insulin increased E2F and PCNA (proliferating cell nuclear antigen) transcription after 15 h of germination and PCNA de novo synthesis at 15 h of germination. These results show that bovine insulin preferentially stimulates growth in the radicles of germinating embryonic axes and suggest that its effect on the G1–S transition and the activation of cell proliferation is mediated by the induction of E2F and PCNA transcription.

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Section: Introductionmentioning

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Effect of insulin on the cell cycle of germinating maize seeds (Zea maysL.)

et al. 2013

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“…Further, analysis of the regulation of expression related to ribosomal protein [(r)-protein] mRNAs was pursued, considering that expression of these genes constitutes the platform for the embryo to grow and develop. Previous reports demonstrated that insulin or insulin-like growth factors are able to stimulate growth in most eukaryotes (Fingar andBlenis 2004, Leevers andHafen 2004), including several plant species during seed germination (Goodman and Davis 1993, García-Flores et al 2001, Oliveira et al 2004. Furthermore, accelerated (r)-protein synthesis has been found following either insulin or maize insulin-like growth factor (ZmIGF; Rodríguez-López et al 2011) stimulation, in germinating maize seeds (García-Flores et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Regulation of Gene Expression

Singh¹,

Yaster²,

Guan³

et al. 2013

Encyclopedia of Systems Biology

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Seed germination is a critical developmental period for plant propagation. Information regarding gene expression within this important period is relevant for understanding the main biochemical processes required for successful germination, particularly in maize, one of the most important cereals in the world. The present research focuses on the global microarray analysis of differential gene expression between quiescent and germinated maize embryo stages. This analysis revealed that a large number of mRNAs stored in the quiescent embryonic axes (QEAs) were differentially regulated during germination in the 24 h germinated embryonic axes (GEAs). These genes belong to 14 different functional categories and most of them correspond to metabolic processes, followed by transport, transcription and translation. Interestingly, the expression of mRNAs encoding ribosomal proteins [(r)-proteins], required for new ribosome formation during this fast-growing period, remains mostly unchanged throughout the germination process, suggesting that these genes are not regulated at the transcriptional level during this developmental period. To investigate this issue further, comparative microarray analyses on polysomal mRNAs from growth-stimulated and non-stimulated GEAs were performed. The results revealed that (r)-protein mRNAs accumulate to high levels in polysomes of the growth-stimulated tissues, indicating a translational control mechanism to account for the rapid (r)-protein synthesis observed within this period. Bioinformatic analysis of (r)-protein mRNAs showed that 5 0 TOP (tract of pyrimidines)-like sequences are present only in the 5 0-untranslated region set of up-regulated (r)-protein mRNAs. This overall approach to the germination process allows an in-depth view of molecular changes, enabling a broader understanding of the regulatory mechanisms that occur during this process.

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“…Further, analysis of the regulation of expression related to ribosomal protein [(r)-protein] mRNAs was pursued, considering that expression of these genes constitutes the platform for the embryo to grow and develop. Previous reports demonstrated that insulin or insulin-like growth factors are able to stimulate growth in most eukaryotes (Fingar andBlenis 2004, Leevers andHafen 2004), including several plant species during seed germination (Goodman and Davis 1993, García-Flores et al 2001, Oliveira et al 2004). Furthermore, accelerated (r)-protein synthesis has been found following either insulin or maize insulin-like growth factor (ZmIGF; Rodríguez-López et al 2011) stimulation, in germinating maize seeds (García-Flores et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Regulation of Gene Expression

Tomkins¹

2013

Encyclopedia of Biophysics

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Seed germination is a critical developmental period for plant propagation. Information regarding gene expression within this important period is relevant for understanding the main biochemical processes required for successful germination, particularly in maize, one of the most important cereals in the world. The present research focuses on the global microarray analysis of differential gene expression between quiescent and germinated maize embryo stages. This analysis revealed that a large number of mRNAs stored in the quiescent embryonic axes (QEAs) were differentially regulated during germination in the 24 h germinated embryonic axes (GEAs). These genes belong to 14 different functional categories and most of them correspond to metabolic processes, followed by transport, transcription and translation. Interestingly, the expression of mRNAs encoding ribosomal proteins [(r)-proteins], required for new ribosome formation during this fast-growing period, remains mostly unchanged throughout the germination process, suggesting that these genes are not regulated at the transcriptional level during this developmental period. To investigate this issue further, comparative microarray analyses on polysomal mRNAs from growth-stimulated and non-stimulated GEAs were performed. The results revealed that (r)-protein mRNAs accumulate to high levels in polysomes of the growth-stimulated tissues, indicating a translational control mechanism to account for the rapid (r)-protein synthesis observed within this period. Bioinformatic analysis of (r)-protein mRNAs showed that 5 0 TOP (tract of pyrimidines)-like sequences are present only in the 5 0 -untranslated region set of up-regulated (r)-protein mRNAs. This overall approach to the germination process allows an in-depth view of molecular changes, enabling a broader understanding of the regulatory mechanisms that occur during this process.The microarray database was registered in GEO, with series record GSE27648. For information on GEO linking: http:// www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE27648

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Insulin Accelerates Seedling Growth of Canavalia ensiformis (Jack bean)

Cited by 16 publications

References 32 publications

Effect of insulin on the cell cycle of germinating maize seeds (Zea maysL.)

Effect of insulin on the cell cycle of germinating maize seeds (Zea maysL.)

Regulation of Gene Expression

Regulation of Gene Expression

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