Instrumental Insemination of Honey Bee Queens During Flight Activity Predisposition Period 2. Number of Spermatozoa in Spermatheca

Gerula, Dariusz; Panasiuk, Beata; Węgrzynowicz, Paweł; Bieńkowska, Małgorzata

doi:10.2478/v10289-012-0016-8

Cited by 10 publications

(8 citation statements)

References 9 publications

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“…2). Our results also agreed with reports found in A. mellifera where number of stored sperm from instrumentally inseminated queen was not significantly lower than that of naturally mated queens (Cobey, 2007;Gerula et al, 2012).…”

Section: Discussionsupporting

confidence: 93%

Impact of Confinement and Population Size on the Instrumentally Inseminated Queen’s Performance of Apis cerana Species in South Korea

Vung¹,

Kim²,

Lee³

et al. 2018

apiculture

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251Instrumental insemination of honey bee is an attractive alternative to natural mating in breeding program as it allows mating crosses between desirable queen and specific drone. However, nursery condition that the queen is kept before and after insemination is major factor affected to the performance of instrumentally inseminated queen. In this study, we evaluated the influences of three different nursery-conditions of push-in cages, mini nuclei and normal colonies on number of spermatozoa stored in the spermatheca, body weight, onset of ovipositon and performance of instrumentally inseminated Apis cerana queen. Our results demonstrated that instrumentally inseminated queens kept in mini nuclei and in normal hives showed no significant difference in queen's weight (159.8 and 166.2mg, respectively), number of spermatozoa in spermatheca (2.02 10 6 and 2.76 10 6 , respectively), proportion of queen supersedure (33.3 and 66.7% queen survival at 11 months after oviposition, respectively) and brood production, compared to naturally mated queens. In contrast, instrumentally inseminated queens kept in push-in cages showed significant difference of those above data in comparison to queens mated naturally. Our results suggested that instrumentally inseminated queens could be kept in mini nuclei containing about 1.000 attendant bees to have desirable performance of queen whereas the push-in method should be practiced for the purpose of using queen in the length of time less than 7 months. Apiculture 33(4) : 251~260 (2018) Abstract Journal of

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Section: Discussionsupporting

confidence: 93%

Impact of Confinement and Population Size on the Instrumentally Inseminated Queen’s Performance of Apis cerana Species in South Korea

Vung¹,

Kim²,

Lee³

et al. 2018

apiculture

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“…In any case, further investigations are needed to highlight the extent of this phenomenon and to understand its causes. Substantial discrepancies in the average number of sperm found in each spermatheca are reported in the literature: 1 -8 million in queens just mated, with an average of 3.5 million during the life of the queen (Al-Lawati et al, 2009); 4.7 -5.3 million (Gerula et al, 2012); over 7 million (Bieńkowska et al, 2011). The average sperm number observed by us in newly mated queens, indicates that inadequate mating and/or that defective sperm transfer into the spermatheca occurred at least in a substantial part of the sampled queens as the figures of the first and the second quartiles suggest: 1,184,896 and 1,994,792 sperms, respectively.…”

Section: Discussionmentioning

confidence: 99%

Survey of the Health Status of Some Honey Bee Queens in Italy

Porporato

Grillone

Patetta

et al. 2015

Journal of Apicultural Science

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A b s t r a c t While observing: non-acceptance, frequent replacements, and reduced performance in honey bee (Apis mellifera ligustica) queens, we were induced to analyse a certain number of queens to detect the causes. For this purpose, 99 newly mated queens were bought from 20 Italian queen breeders. In addition, 109 older or at-the-end-of-their-career queens, that showed poor productivity, were collected from honey production hives throughout Italy. All the queens were dissected to check the status of their reproductive system and/or the presence of various anomalies and diseases. Anatomical and functional anomalies, pertaining to ovaries and spermatheca, also in association with tissue alterations and microorganisms like Nosema spp. and protozoa, were common in both newly mated and older queens, but they were more prevalent in the latter. Observed differences permitted us to highlight the causes of the reduced performance of the queens and of the colonies they originated from. The obtained information could prove useful when it comes to improving queen breeding techniques.

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“…Studies on honey bee queen mating thus far suggest that carbon dioxide (CO 2 ; the anaesthetic used in instrumental insemination), physical manipulation of the genital opening, seminal volume, number of spermatozoa in spermatheca, and possibly components of the seminal fluid all play a role in causing post‐mating changes of NM queens, inseminated queens, or both (Richard et al ., ; Kocher et al ., , , ; Niño et al ., ; Gerula et al ., ). Previously, we demonstrated that CO 2 and physical manipulation of the genital opening alter queen sexual receptivity (as measured by mating flight attempts), induce ovary activation, and modify gene expression in the brain (Niño et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Differential effects of insemination volume and substance on reproductive changes in honey bee queens (Apis mellifera L.)

Niño

Grozinger

2013

Insect Molecular Biology

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Mating causes dramatic changes in female insects at the behavioural, physiological and molecular level. The factors driving these changes (e.g. seminal proteins, seminal volume) and the molecular pathways by which these factors are operating have been characterized only in a handful of insect species. In the present study, we use instrumental insemination of honey bee queens to examine the role of the insemination substance and volume in triggering post-mating changes. We also examine differences in gene expression patterns in the fat bodies of queens with highly activated ovaries to determine if events during copulation can cause long-term changes in gene expression. We found that the instrumental insemination procedure alone caused cessation of mating flights and triggered ovary activation, with high-volume inseminated queens having the greatest ovary activation. Hierarchical clustering grouped queens primarily by insemination substance and then insemination volume, suggesting that while volume may trigger short-term physiological changes (i.e. ovary activation) substance plays a greater role in regulating long-term transcriptional changes. The results of gene ontology analysis and comparison with previous studies suggest that both insemination substance and volume trigger molecular post-mating changes by altering overlapping gene pathways involved in honey bee reproduction. We also discuss the effects on two genes (vitellogenin and transferrin) involved in reproduction and defence responses.

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Instrumental Insemination of Honey Bee Queens During Flight Activity Predisposition Period 2. Number of Spermatozoa in Spermatheca

Cited by 10 publications

References 9 publications

Impact of Confinement and Population Size on the Instrumentally Inseminated Queen’s Performance of Apis cerana Species in South Korea

Impact of Confinement and Population Size on the Instrumentally Inseminated Queen’s Performance of Apis cerana Species in South Korea

Survey of the Health Status of Some Honey Bee Queens in Italy

Differential effects of insemination volume and substance on reproductive changes in honey bee queens (Apis mellifera L.)

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