Instrumental improvements for the trace analysis of structural isomers of polycyclic aromatic hydrocarbons with molecular mass 302 Da

On the co-elution of benzo[a]pyrene and dibenzo[a,l]pyrene in chromatographic fractions and their unambiguous determination in tobacco extracts via laser-excited time resolved Shpol'skii spectroscopy

“…Since the LETRSS analysis of N23 kF and DahP in co-eluted HPLC fractions was already reported from our lab. 36 Spectral resolution of N23 kF and DahP was achieved by freezing the sample at liquid helium temperature (4.2 K). The present studies will focus on BaP and DBalP and their LETRSS analysis at 77 K. Although better spectral resolution and lower limits of detection are usually obtained at 4.2 K, the analysis of samples at liquid nitrogen temperature (77 K) present the advantage of lower analysis costs.…”

“…LETRSS measurements were performed with an instrumental set-up built in-house. 36 A Radiant 355 LD UV tunable laser system from OPOTek Inc. w with tuning capability from 210 to 2500 nm was used as the excitation source. The laser power was controlled with a half-wave plate, a polarizer cube, and a neutral density filter.…”