Instrumental analysis of bacterial cells using vibrational and emission Mössbauer spectroscopic techniques

Kamnev, Alexander A.; Tugarova, Anna V.; Antonyuk, L. P.; Tarantilis, Petros Α.; Куликов, Л. А.; Perfiliev, Yu. D.; Polissiou, Moschos G.; Gardiner, Philip H. E.

doi:10.1016/j.aca.2006.04.041

Cited by 32 publications

(20 citation statements)

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“…Naumann Naumann et al, 1991) elegantly made use of this feature to identify several classes of bacteria by applying cluster analysis to the infrared spectra of these bacteria. Infrared spectroscopy was also applied to monitor qualitative changes in micro-organisms, introduced by the incorporation of heavy metals (Gupta and Rastogi, 2008;Kamnev et al, 2006). However, due to the large diversity, a quantitative estimation of biomass composition cannot be easily achieved.…”

Section: Introductionmentioning

confidence: 99%

Monitoring of biomass composition from microbiological sources by means of FT‐IR spectroscopy

Pistorius¹,

DeGrip²,

Egorova-Zachernyuk³

2009

Biotech & Bioengineering

153

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An FT-IR spectroscopic method was developed for the simultaneous quantitative analysis of biomacromolecular components in biomass, originating from various microbiological sources. For the determination of protein, lipid and carbohydrate content, creatine phosphokinase, egg phosphatidyl choline and starch hydrolysate were chosen as external standards. This selection was based on spectral similarity and ease of availability. Protein content was based on the area under the amide II band profile around 1,545 cm(-1). Because of the heterogeneous lipid composition in the different species, lipid content was determined using integration over the C-H stretching vibrational population between 2,984 and 2,780 cm(-1). Carbohydrate content was determined using integration over a C-O and C-O-C stretching band area between 1,180 and 1,133 cm(-1). Linear regression analysis provided three calibration lines, according to which biomasses from ten species were analyzed. This approach showed good intra-batch reproducibility. With this method we could demonstrate good reproducibility between batches of the same species with similar growth conditions while large differences in biomass composition were observed between the various species. Protein content as determined by FT-IR spectroscopy compared well with the results obtained from elemental analysis.

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Section: Introductionmentioning

confidence: 99%

Monitoring of biomass composition from microbiological sources by means of FT‐IR spectroscopy

Pistorius¹,

DeGrip²,

Egorova-Zachernyuk³

2009

Biotech & Bioengineering

153

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“…In this preliminary work, 57 Co emission Mössbauer spectra were measured for live and dead cells of A. brasilense strain Sp7 at T = 80 K in frozen 57 Co 2+ -containing aqueous suspensions and for their dried residues. The results are compared with similarly obtained data reported earlier for A. brasilense strain Sp245 [4].…”

Section: Introductionmentioning

confidence: 58%

“…Interaction of bacterial cells with cobalt ions, besides its scientific aspects [2][3][4], is also of ecological significance [5]. Table 1, samples "Live (2 min), D" and "Live (1 h), D") are shown above the spectra (the third light-shaded doublets correspond to daughter 57 Fe III resulting from after-effects)…”

Section: Introductionmentioning

confidence: 99%

“…Its strain Sp245 is a facultative endophyte (capable of penetrating into and colonising the plant root tissues), while strain Sp7 is an epiphyte (colonising the root surface only). Thus, the two strains of the same species occupy different ecological niches and can show differences in behaviour under similar conditions [4].…”

Section: Introductionmentioning

confidence: 99%

“…Emission ( 57 Co) Mössbauer spectroscopy is a sensitive and informative tool, which has scarcely been used in biology-related studies [2,4,7]. In this preliminary work, 57 Co emission Mössbauer spectra were measured for live and dead cells of A. brasilense strain Sp7 at T = 80 K in frozen 57 Co 2+ -containing aqueous suspensions and for their dried residues.…”

Section: Introductionmentioning

confidence: 99%

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Co2 + interaction with Azospirillum brasilense Sp7 cells: a 57Co emission Mössbauer spectroscopic study

et al. 2012

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Preliminary 57 Co emission Mössbauer spectroscopic data were obtained for the soil bacterium Azospirillum brasilense Sp7 (T = 80 K) in frozen 57 Co 2+ -containing suspensions and in their dried residues. The Mössbauer parameters were compared with those for A. brasilense strain Sp245 differing from strain Sp7 by ecological behaviour. Live cells of both strains showed metabolic transformations of 57 Co 2+ within an hour. Differences in the parameters observed for the two strains under similar conditions suggest dissimilarities in their metabolic response to Co 2+ .

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Emission ( ⁵⁷ Co) Mössbauer Spectroscopy: Biology‐Related Applications, Potentials, and Prospects

Kamnev

2013

Mössbauer Spectroscopy

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Instrumental analysis of bacterial cells using vibrational and emission Mössbauer spectroscopic techniques

Cited by 32 publications

References 42 publications

Monitoring of biomass composition from microbiological sources by means of FT‐IR spectroscopy

Monitoring of biomass composition from microbiological sources by means of FT‐IR spectroscopy

Co2 + interaction with Azospirillum brasilense Sp7 cells: a 57Co emission Mössbauer spectroscopic study

Emission ( ⁵⁷ Co) Mössbauer Spectroscopy: Biology‐Related Applications, Potentials, and Prospects

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Instrumental analysis of bacterial cells using vibrational and emission Mössbauer spectroscopic techniques

Cited by 32 publications

References 42 publications

Monitoring of biomass composition from microbiological sources by means of FT‐IR spectroscopy

Monitoring of biomass composition from microbiological sources by means of FT‐IR spectroscopy

Co2 + interaction with Azospirillum brasilense Sp7 cells: a 57Co emission Mössbauer spectroscopic study

Emission ( 57 Co) Mössbauer Spectroscopy: Biology‐Related Applications, Potentials, and Prospects

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Emission ( ⁵⁷ Co) Mössbauer Spectroscopy: Biology‐Related Applications, Potentials, and Prospects