Instrument-Free and Visual Detection of Salmonella Based on Magnetic Nanoparticles and an Antibody Probe Immunosensor

Zhang, Liding; Du, Xuewei; Chen, Zhuo; Chen, Congjie; Gong, Nanxin; Song, Yihao; Song, Yuzhu; Han, Qinqin; Xia, Xueshan; Luo, Haiming; Zhang, Jinyang

doi:10.3390/ijms20184645

Cited by 12 publications

(3 citation statements)

References 77 publications

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“…A portable, compact, homoiothermal instrument was considered for visual inspection of the RPA amplification product. In view of the market prospects, RPA, in combination with a lateral flow dipstick (LFD), is the ideal choice for point-of-care detection in a resource-limited setting [26]. The LFD is a simple device for endpoint testing with an antibody specific to an antigen, in which colloidal gold is commonly used for labelling.…”

Section: Introductionmentioning

confidence: 99%

Recombinase Polymerase Amplification (RPA) Combined with Lateral Flow Immunoassay for Rapid Detection of Salmonella in Food

Fang

et al. 2019

Foods

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Salmonella can cause serious foodborne diseases. We have developed a lateral flow immunoassay combined with recombinase polymerase amplification (LFD-RPA) for detection of Salmonella in food. The conserved fragment (fimY) was selected as the target gene. Under an optimal condition (37 °C, 10 min), the sensitivity was 12 colony-forming units (CFU)/mL in a pure culture. Testing with 16 non-Salmonella strains as controls revealed that LFD-RPA was specific to the fimY gene of Salmonella. The established assay could detect Salmonella at concentrations as low as 1.29 × 102 CFU/mL in artificially contaminated samples. This detection was at a slightly higher level than that for a pure bacterial culture. Combined with the test strip reader, the LFD-RPA is a feasible method for quantitative detection of Salmonella based on the test line intensity, which was the ratio for the test line and control line of the reflected light. The method could be a potential point-of-care test in limited resource areas and provides a new approach and technical support for the diagnosis of food safety.

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Section: Introductionmentioning

confidence: 99%

Recombinase Polymerase Amplification (RPA) Combined with Lateral Flow Immunoassay for Rapid Detection of Salmonella in Food

Fang

et al. 2019

Foods

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“…ELISA-based detection has been employed in a variety of formats, mostly immunofluorescence and lateral flow immunochromatography, as well as in the development of immunosensors [ 33 , 34 , 35 , 36 , 37 , 38 , 39 ]. In all cases, the attractive features are ease of use, portability and speed of analysis.…”

Section: Surface-residing Molecular Targetsmentioning

confidence: 99%

Molecular Targets for Foodborne Pathogenic Bacteria Detection

Paramithiotis

2023

Pathogens

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The detection of foodborne pathogenic bacteria currently relies on their ability to grow on chemically defined liquid and solid media, which is the essence of the classical microbiological approach. Such procedures are time-consuming and the quality of the result is affected by the selectivity of the media employed. Several alternative strategies based on the detection of molecular markers have been proposed. These markers may be cell constituents, may reside on the cell envelope or may be specific metabolites. Each marker provides specific advantages and, at the same time, suffers from specific limitations. The food matrix and chemical composition, as well as the accompanying microbiota, may also severely compromise detection. The aim of the present review article is to present and critically discuss all available information regarding the molecular targets that have been employed as markers for the detection of foodborne pathogens. Their strengths and limitations, as well as the proposed alleviation strategies, are presented, with particular emphasis on their applicability in real food systems and the challenges that are yet to be effectively addressed.

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“…Molecular biology methods achieve the detection of pathogens with high accuracy and sensitivity, such as gene chip technology, gene probes, polymerase chain reaction (PCR) (Arunrut et al, 2018), and loopmediated isothermal amplification (LAMP) , but these methods have higher detection cost and requirements on the professionalism of equipment and operators (Wang et al, 2018). Based on antigen-antibody specific binding capacity, immunological detection technology has been widely used for bacterial detection in the recent years, by mainly targeting the surface antigens of pathogenic bacteria (Zhang et al, 2019). Current common immunoassay-methods include enzyme-linked immunosorbent assay (ELISA), chemiluminescent enzyme immunoassay (CLEIA), immunomagnetic bead separation (IMS), and immunofluorescence labelling technology (IFT) (Zhu et al, 2011;Hyeon et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Development of IMBs-qPCR method for detection of foodborne Salmonella

Sun,

Cao,

et al. 2023

IFRJ

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Rapid and accurate detection of pathogenic microorganism is critical for food safety. Salmonella is one of the common causes of food poisoning. In the present work, polyclonal antibody against the recombinant PagN protein was prepared, and coupled with carboxylated magnetic beads to form immunomagnetic beads (IMBs) for capturing Salmonella, which was then combined with qPCR technology which used the specific primers of invA gene to accurately quantify the number of colonies, thus establishing the IMBs-qPCR method for detection of Salmonella. 0.2 mg IMBs could specifically concentrate Salmonella, with the stable capturing efficiency of 80%, corresponding to the concentrations of 102 - 105 CFU/mL. The minimum detection limit concentration was 101 CFU/mL. The method was applied for detection and enumeration of Salmonella in pork and milk samples, and the capture efficiency of 77.38 and 80.92% were obtained. In summary, the IMBs-qPCR method established herein could effectively detect Salmonella with good specificity and sensitivity. The whole detection time was less than 9 h, which laid a foundation for development of a rapid detection kit for foodborne pathogens.

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Instrument-Free and Visual Detection of Salmonella Based on Magnetic Nanoparticles and an Antibody Probe Immunosensor

Cited by 12 publications

References 77 publications

Recombinase Polymerase Amplification (RPA) Combined with Lateral Flow Immunoassay for Rapid Detection of Salmonella in Food

Recombinase Polymerase Amplification (RPA) Combined with Lateral Flow Immunoassay for Rapid Detection of Salmonella in Food

Molecular Targets for Foodborne Pathogenic Bacteria Detection

Development of IMBs-qPCR method for detection of foodborne Salmonella

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