Instillation of Taurolidine/Heparin after Laparotomy Reduces Intraperitoneal Tumour Growth in a Colon Cancer Rat Model

Abstract

Objective: To investigate whether irrigation of the abdominal cavity after laparotomy for caecum resection with taurolidine/heparin or adhesion prophylactic substances reduces intraperitoneal tumour growth or the local recurrence rate in a colon carcinoma rat model. Methods: 60 BDIX rats underwent caecum resection after intraperitoneal inoculation of 1 × 10⁴ colon carcinoma cells (DHD/K12/TRb). Intergel®, Interceed®, taurolidine/heparin or NaCl 0.9% were intraperitoneally applied after randomisation. Finally, the total number and total weight of intraperitoneal metastases were determined as well as the adhesion score according to Moreno. Metastatic tissue was examined histologically and immunohistochemically (E-cadherin, CD44, β₁-integrin). Results: Taurolidine/heparin significantly reduced not only the total number (3 vs. 11 in the control group) but also the total weight (65 vs. 330 mg) of intraperitoneal metastases in comparison to the control group (p = 0.003 and p = 0.005). E-Cadherin expression in the metastatic tissue of animals treated with taurolidine/heparin was significantly decreased (p = 0.016). Conclusion: Taurolidine/heparin effectively reduces intraperitoneal tumour growth when used as an intraoperative lavage. These results represent a good rationale for intraoperative adjuvant irrigation with taurolidine/heparin during resection of colon cancer.

Oral administration of the anti-proliferative substance taurolidine has no impact on dextran sulfate sodium-induced colitis-associated carcinogenesis in mice

“…TRD has been shown to mediate anti-proliferative and anti-neoplastic effects towards different colon carcinoma cell lines in vitro [192443–47] as well as in vivo in different animal models of colon carcinoma with i.p. [44–4648–53] or i.v. application[54–58] of TRD.…”

An Assessment of the Effects of a Hyaluronan-Based Solution on Reduction of Postsurgical Adhesion Formation in Rats: A Comparative Study of Hyaluronan-Based Solution and Two Film Barriers

“…Two surgeons and one pathologist, blind to the study purpose, scored the adhesions separately, and a consensus score was obtained for each rat. A previously validated adhesion score system was used to determine the number, site, thickness, tenacity, and vascularization of the intra-abdominal adhesions (Table 1) [13].…”

Comparative analysis of cell death induction by Taurolidine in different malignant human cancer cell lines

“…As a result, additional caspase independent forms of PCD have been suggested like autophagy or necrosis [9]. Furthermore, there is growing evidence from recent publications, that generation of reactive oxygen species (ROS) plays an important role in TRD induced PCD [9,13,24,29]. However, the majority of information about TRD effects is provided from studies with one single cell line or several cell lines of one single malignancy.…”