Instantaneous topical drug quantification using a 3D printed microfluidic device and coherent Raman imaging

Quantitative analysis of drug tablet aging by fast hyper-spectral stimulated Raman scattering microscopy

“…We have previously applied S 4 RS for monitoring spatiotemporal dynamics of topical drug delivery in mouse skin. 29,57 Here, we demonstrate the utility of S 4 RS to achieve hyperspectral unmixing of components with high spectral overlap with rapid acquisition of multiple fields of view, along with full tablet cross-sectional imaging. We conduct quantitative analysis of API and excipient distributions to uncover spatially heterogeneous chemical instability at stress conditions.…”

Innovative Approaches for Drug Discovery: Quantifying Drug Distribution and Response with Raman Imaging

“…Kuzma et al further studied cutaneous pharmacokinetics through the development of a 3D-printed microfluidic device compatible with CRS imaging for relative concentration monitoring following drug product application. 113 This platform was utilized to measure ruxolitinib uptake concentration−time profiles of two formulations into layers of frozen nude mouse ear skin. Recently, Iliopoulos et al employed SRS to assess the permeation of tazarotene, a topical retinoid, through various skin microstructures.…”

“…By monitoring drug distribution over time, they were able to quantitatively track the drug penetration kinetics through different skin layers. Kuzma et al further studied cutaneous pharmacokinetics through the development of a 3D-printed microfluidic device compatible with CRS imaging for relative concentration monitoring following drug product application . This platform was utilized to measure ruxolitinib uptake concentration–time profiles of two formulations into layers of frozen nude mouse ear skin.…”

Optical Methods for Non-Invasive Determination of Skin Penetration: Current Trends, Advances, Possibilities, Prospects, and Translation into In Vivo Human Studies

“…Here, the “Stokes” beam at 803 nm targets lipids at 2845 cm −1 and the “pump” beam at 845 nm targets the nitrile stretch of ruxolitinib at 2220 cm −1 (both excitation wavelengths lie in the “skin optical transparent window-I” ( Figure 3 )). The in vivo SRS imaging of mammalian skin constituents and topically applied formulations is possible [ 283 , 284 , 285 , 286 ] and very prospective, but is not considered due to the high cost and complexity of the equipment and its limitations for safe application on human skin [ 273 ].…”