Inspired by Nature: The use of Plant-derived Substrate/Enzyme Combinations to Generate Antimicrobial Activity<i>in situ</i>

Estevam, Ethiene Castellucci; Griffin, Sharoon; Nasim, Muhammad Jawad; Zieliński, Dariusz; Aszyk, Justyna; Osowicka, Magdalena; Dawidowska, Natalia; Idroes, Rinaldi; Bartoszek, Agnieszka; Jacob, Claus

doi:10.1177/1934578x1501001025

Cited by 24 publications

(21 citation statements)

References 35 publications

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“…In the work , the alliin/alliinase system and the glucosinolate/myrosinase system were effective against E. coli , Saccharomyces cerevisiae , Staphylococcus carnosus , and Steinernema feltiae . The MICs values of the alliin/alliinase couple against E. coli and S. carnosus were evaluated at 100 μg/mL .…”

Section: Resultsmentioning

confidence: 97%

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Mutant formC115HofClostridium sporogenesmethionine γ‐lyase efficiently cleavesS‐Alk(en)yl‐l‐cysteine sulfoxides to antibacterial thiosulfinates

et al. 2016

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Pyridoxal 5'-phosphate-dependent methionine γ-lyase (MGL) catalyzes the β-elimination reaction of S-alk(en)yl-l-cysteine sulfoxides to thiosulfinates, which possess antimicrobial activity. Partial inactivation of the enzyme in the course of the reaction occurs due to oxidation of active site cysteine 115 conserved in bacterial MGLs. In this work, the C115H mutant form of Clostridium sporogenes MGL was prepared and the steady-state kinetic parameters of the enzyme were determined. The substitution results in an increase in the catalytic efficiency of the mutant form towards S-substituted l-cysteine sulfoxides compared to the wild type enzyme. We used a sulfoxide/enzyme system to generate antibacterial activity in situ. Two-component systems composed of the mutant enzyme and three S-substituted l-cysteine sulfoxides were demonstrated to be effective against Gram-positive and Gram-negative bacteria and three clinical isolates from mice. © 2016 IUBMB Life, 68(10):830-835, 2016.

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Section: Resultsmentioning

confidence: 97%

“…To overcome this problem, systems for the in situ generation of allicin by alliinase conjugated with anti‐ Aspergillus fumigatus monoclonal antibodies were elaborated and tested . Allicin generated in situ by the alliinase/substrate couple was shown to possess antifungal , antimicrobial and anti‐yeast activities.…”

Section: Introductionmentioning

confidence: 99%

Mutant formC115HofClostridium sporogenesmethionine γ‐lyase efficiently cleavesS‐Alk(en)yl‐l‐cysteine sulfoxides to antibacterial thiosulfinates

et al. 2016

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“…The nanosized and stabilized particles were suspended in distilled water and then tested against three model organisms, namely the Gram-negative bacterium Escherichia coli , the yeast Saccharomyces cerevisiae and the agricultural nematode Steinernema feltiae [ 13 ]. Nematicidal assays were performed in the morning and antimicrobial assays in the early afternoon.…”

Section: Methodsmentioning

confidence: 99%

Turning Waste into Value: Nanosized Natural Plant Materials of Solanum incanum L. and Pterocarpus erinaceus Poir with Promising Antimicrobial Activities

et al. 2016

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Numerous plants are known to exhibit considerable biological activities in the fields of medicine and agriculture, yet access to their active ingredients is often complicated, cumbersome and expensive. As a consequence, many plants harbouring potential drugs or green phyto-protectants go largely unnoticed, especially in poorer countries which, at the same time, are in desperate need of antimicrobial agents. As in the case of plants such as the Jericho tomato, Solanum incanum, and the common African tree Pterocarpus erinaceus, nanosizing of original plant materials may provide an interesting alternative to extensive extraction and isolation procedures. Indeed, it is straightforward to obtain considerable amounts of such common, often weed-like plants, and to mill the dried material to more or less uniform particles of microscopic and nanoscopic size. These particles exhibit activity against Steinernema feltiae or Escherichia coli, which is comparable to the ones seen for processed extracts of the same, respective plants. As S. feltiae is used as a model nematode indicative of possible phyto-protective uses in the agricultural arena, these findings also showcase the potential of nanosizing of crude “waste” plant materials for specific practical applications, especially—but not exclusively—in developing countries lacking a more sophisticated industrial infrastructure.

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“…Since allicin itself is not particularly stable chemically, similar studies have considered a combination of alliinase and synthetic alliin substrates as a cytotoxic “binary agent”, with possible applications in medicine and agriculture [ 46 ]. Related therapeutic “binary weapons” based on an enzyme and substrate combination include the enzyme myrosinase found in mustard and rapeseeds and its various glucosinolate substrates, which together form a spectrum of highly aggressive isothiocyanates, thiocyanates, nitriles and thiosulfinates [ 32 , 47 , 48 ].…”

Section: The Use Of Enzymes In Therapymentioning

confidence: 99%

Small Molecule Catalysts with Therapeutic Potential

et al. 2018

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Catalysts are employed in many areas of research and development where they combine high efficiency with often astonishing selectivity for their respective substrates. In biology, biocatalysts are omnipresent. Enzymes facilitate highly controlled, sophisticated cellular processes, such as metabolic conversions, sensing and signalling, and are prominent targets in drug development. In contrast, the therapeutic use of catalysts per se is still rather limited. Recent research has shown that small molecule catalytic agents able to modulate the redox state of the target cell bear considerable promise, particularly in the context of inflammatory and infectious diseases, stroke, ageing and even cancer. Rather than being “active” on their own in a more traditional sense, such agents develop their activity by initiating, promoting, enhancing or redirecting reactions between biomolecules already present in the cell, and their activity therefore depends critically on the predisposition of the target cell itself. Redox catalysts, for instance, preferably target cells with a distinct sensitivity towards changes in an already disturbed redox balance and/or increased levels of reactive oxygen species. Indeed, certain transition metal, chalcogen and quinone agents may activate an antioxidant response in normal cells whilst at the same time triggering apoptosis in cancer cells with a different pre-existing “biochemical redox signature” and closer to the internal redox threshold. In pharmacy, catalysts therefore stand out as promising lead structures, as sensor/effector agents which are highly effective, fairly selective, active in catalytic, i.e., often nanomolar concentrations and also very flexible in their structural design.

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Inspired by Nature: The use of Plant-derived Substrate/Enzyme Combinations to Generate Antimicrobial Activityin situ

Cited by 24 publications

References 35 publications

Mutant formC115HofClostridium sporogenesmethionine γ‐lyase efficiently cleavesS‐Alk(en)yl‐l‐cysteine sulfoxides to antibacterial thiosulfinates

Mutant formC115HofClostridium sporogenesmethionine γ‐lyase efficiently cleavesS‐Alk(en)yl‐l‐cysteine sulfoxides to antibacterial thiosulfinates

Turning Waste into Value: Nanosized Natural Plant Materials of Solanum incanum L. and Pterocarpus erinaceus Poir with Promising Antimicrobial Activities

Small Molecule Catalysts with Therapeutic Potential

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