Insilico Functional Annotation of Hypothetical ORFs in Human Chromosome2

Abstract

The high-throughput genome projects have resulted in a rapid accumulation of genome sequences for a large number of organisms and large number of genes with unknown function (Hypothetical). To fully realize the value of the data, scientists need to identify proteins encoded by these genes and understand how these proteins function in making up a living cell. With experimentally verified information on protein function lagging far behind, computational methods are needed for reliable and large-scale functional annotation of proteins. Functional annotation is the process of identifying for a given gene its biological function, interaction with other elements, involvement in metabolic pathways, and any other piece of information that helps in understanding when and how a gene influences the overall system. On the other hand, many Biological Processes and Disease mechanisms are still unknown due to lack of knowledge about the function of the Hypothetical genes in Human. Once its function is revealed the so called hurdle of unknown mechanism of the Human Genome can be mastered. Hence, the present study aims to use computational approaches to annotate the function of hypothetical genes in Chromosome 2 of Human. The annotation of the hypothetical genes in human chromosoem2 was done both at the nucleotide and protein level. Among the 41 uncharacterized hypothetical genes in Human chromosome 2, the functions of 27 of them were successfully annotation. Further, experimental validation is essential to confirm the predicted function.