Insights to antimicrobial resistance: heavy metals can inhibit antibiotic resistance in bacteria isolated from wastewater

Khaira, Massab Bashir; Yusuf, Muhammad Bilal; Khan, Feroz

doi:10.1007/s10661-022-09917-6

Cited by 6 publications

(6 citation statements)

References 50 publications

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“…Paenalcaligenes were also reported to strongly respond to antibiotic exposure [ 30 ]. Interestingly, Carnobacterium was not previously known to possess resistance to tetracycline [ 31 ]. This shift may be due to gene horizontal transfer, which allowed it to acquire the tetracycline resistance genes.…”

Section: Discussionmentioning

confidence: 99%

“…These results indicated that there must be other enzymes in the BSFL gut that presented as tetracycline-degrading enzymes. Furthermore, the analyses of the tetracycline resistance genes revealed that tet (31), tetA(60), tetS, and tetB(46) were the most dominant genes in CK group, with expression levels decreasing to 0.19%, 10.64%, 23.81%, and 10.05% in TC group. Further, the abundances of tet (33), tet(A), tet (B), tet(L), tetA(60), tetR, tetM, and tetW were increased in the TC group compared to the CK group (Figure 4c,d).…”

Section: Antimicrobial Resistance (Amr) Gene Analysismentioning

confidence: 99%

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Comparative Metagenomic and Metatranscriptomic Analyses Reveal the Response of Black Soldier Fly (Hermetia illucens) Larvae Intestinal Microbes and Reduction Mechanisms to High Concentrations of Tetracycline

Pei

Sun

Zhang

et al. 2023

Toxics

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Black soldier fly (Hermetia illucens L) larvae (BSFL) possess remarkable antibiotic degradation abilities due to their robust intestinal microbiota. However, the response mechanism of BSFL intestinal microbes to the high concentration of antibiotic stress remains unclear. In this study, we investigated the shift in BSFL gut microbiome and the functional genes that respond to 1250 mg/kg of tetracycline via metagenomic and metatranscriptomic analysis, respectively. The bio-physiological phenotypes showed that the survival rate of BSFL was not affected by tetracycline, while the biomass and substrate consumption of BSFL was slightly reduced. Natural BSFL achieved a 20% higher tetracycline degradation rate than the germ-free BSFL after 8 days of rearing. Metagenomic and metatranscriptomic sequencing results revealed the differences between the entire and active microbiome. Metatranscriptomic analysis indicated that Enterococcus, Vagococcus, Providencia, and Paenalcaligenes were the active genera that responded to tetracycline. Furthermore, based on the active functional genes that responded to tetracycline pressure, the response mechanisms of BSFL intestinal microbes were speculated as follows: the Tet family that mediates the expression of efflux pumps expel tetracycline out of the microbes, while tetM and tetW release it from the ribosome. Eventually, tetracycline was degraded by deacetylases and novel enzymes. Overall, this study provides novel insights about the active intestinal microbes and their functional genes in insects responding to the high concentration of antibiotics.

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Section: Discussionmentioning

confidence: 99%

Section: Antimicrobial Resistance (Amr) Gene Analysismentioning

confidence: 99%

Comparative Metagenomic and Metatranscriptomic Analyses Reveal the Response of Black Soldier Fly (Hermetia illucens) Larvae Intestinal Microbes and Reduction Mechanisms to High Concentrations of Tetracycline

Pei

Sun

Zhang

et al. 2023

Toxics

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“…Another mechanism is cross-resistance, in which a metal resistance determinant (e.g., a multidrug efflux pump or a particular composition of the cell envelope) is also capable of mediating antibiotic resistance, even in the absence of dedicated antibiotic resistance genes [ 98 ]. At the same time, metal resistance determinants can also increase a bacterium’s sensitivity to certain antibiotics [ 99 ]. Clearly, the potential interactions of copper and other antimicrobials are multifaceted and constitute an area of active research.…”

Section: Discussionmentioning

confidence: 99%

Copper microenvironments in the human body define patterns of copper adaptation in pathogenic bacteria

2022

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Copper is an essential micronutrient for most organisms that is required as a cofactor for crucial copper-dependent enzymes encoded by both prokaryotes and eukaryotes. Evidence accumulated over several decades has shown that copper plays important roles in the function of the mammalian immune system. Copper accumulates at sites of infection, including the gastrointestinal and respiratory tracts and in blood and urine, and its antibacterial toxicity is directly leveraged by phagocytic cells to kill pathogens. Copper-deficient animals are more susceptible to infection, whereas those fed copper-rich diets are more resistant. As a result, copper resistance genes are important virulence factors for bacterial pathogens, enabling them to detoxify the copper insult while maintaining copper supply to their essential cuproenzymes. Here, we describe the accumulated evidence for the varied roles of copper in the mammalian response to infections, demonstrating that this metal has numerous direct and indirect effects on immune function. We further illustrate the multifaceted response of pathogenic bacteria to the elevated copper concentrations that they experience when invading the host, describing both conserved and species-specific adaptations to copper toxicity. Together, these observations demonstrate the roles of copper at the host–pathogen interface and illustrate why bacterial copper detoxification systems can be viable targets for the future development of novel antibiotic drug development programs.

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“…29,30 Importantly, there are many examples of co-and crossresistance between metals and antibiotics in both clinical and environmental settings. [31][32][33][34] Co-resistance occurs when antibiotic and metal resistance genes are located on the same mobile genetic element (i.e., plasmid, transposon, integron). [35][36][37][38][39][40][41][42] When the cell experiences either antibiotic or metal selection pressure, this genetic material is passed on via horizontal gene transfer, giving the recipient organism the required machinery to cope with both stressors.…”

Section: Bacterial Toxicity: Response To Metalsmentioning

confidence: 99%

“…Importantly, there are many examples of co- and cross-resistance between metals and antibiotics in both clinical and environmental settings. 31–34 Co-resistance occurs when antibiotic and metal resistance genes are located on the same mobile genetic element ( i.e. , plasmid, transposon, integron).…”

Section: Bacterial Toxicity: Response To Metalsmentioning

confidence: 99%

Modern materials provoke ancient behavior: bacterial resistance to metal nanomaterials

Mitchell,

Hudson-Smith,

Sharan

et al. 2024

Environ. Sci.: Nano

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Insights to antimicrobial resistance: heavy metals can inhibit antibiotic resistance in bacteria isolated from wastewater

Cited by 6 publications

References 50 publications

Comparative Metagenomic and Metatranscriptomic Analyses Reveal the Response of Black Soldier Fly (Hermetia illucens) Larvae Intestinal Microbes and Reduction Mechanisms to High Concentrations of Tetracycline

Comparative Metagenomic and Metatranscriptomic Analyses Reveal the Response of Black Soldier Fly (Hermetia illucens) Larvae Intestinal Microbes and Reduction Mechanisms to High Concentrations of Tetracycline

Copper microenvironments in the human body define patterns of copper adaptation in pathogenic bacteria

Modern materials provoke ancient behavior: bacterial resistance to metal nanomaterials

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