Insights of Leishmania (Viannia) braziliensis infection in golden hamster (Mesocricetus auratus) intestine

Santos, Amanda Gubert Alves dos; Lima, Lainy Leiny de; Mota, Camila Alves; Góis, Marcelo Biondaro; Fernandes, Andrea Claudia Bekner Silva; Silveira, Thaís Gomes Verzignassi; Sant’Ana, Débora de Mello Gonçales; Melo, Gessilda de Alcântara Nogueira de

doi:10.1016/j.biopha.2018.07.120

Cited by 12 publications

(29 citation statements)

References 40 publications

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“…However, the XID and XID + B1 groups showed an increased depth of the crypts. Deeper crypts indicate an increase in cell proliferation and renewal, which is associated with the increase of the villi width in these groups . High cell proliferation negatively modifies the differentiation of epithelial cells, as observed with enterocytes in the present study.…”

Section: Discussionsupporting

confidence: 76%

“…A reduction in depth and width of the crypts in Balb/c I group also occurred, which can be related to the immune cell infiltrate found in the crypts. This result, associated with the increase in the villi width, shows a greater innate response in the organ after infection . However, the XID and XID + B1 groups showed an increased depth of the crypts.…”

Section: Discussionmentioning

confidence: 78%

“…The thickness of the total ileum wall, muscular and submucosa, the width and depth of the crypts, and height and width of the villi of each animal were measured; 16 images were captured with a 20X objective lens (1 image/quadrant/cut). The Image Pro Plus software (Media Cybernetics) was used to obtain 64 measurements of each parameter …”

Section: Methodsmentioning

confidence: 99%

“…Therefore, the goblet cells from each animal were counted along the 2560 epithelial cells. Then, the proportion of these cells in relation to 100 epithelial cells was calculated …”

Section: Methodsmentioning

confidence: 99%

“…After laparotomy, the ileum was fixed in paraformaldehyde and em- used to obtain 64 measurements of each parameter. [31][32][33] The height and width of 80 enterocytes and their nuclei/animal were measured by capturing images of the intestinal epithelium with a 100X objective lens (1 image/quadrant/cut). The Paneth cells were counted in four random crypts per quadrant of four cuts per animal, totalizing 64 crypts.…”

Section: Histological Analysesmentioning

confidence: 99%

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Infection by Leishmania (Leishmania) infantum chagasi causes intestinal changes B‐1 cells dependent

Souza

Fernandes

Santos

et al. 2019

Parasite Immunology

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Evaluating the histopathological and morphometric changes caused by Leishmania (Leishmania) infantum chagasi infection either in the presence or absence of B‐1 cells. Wild‐type Balb/c and XID mice were used. Half of XID mice received B‐1 cells adoptive transfer (XID + B1). Five animals from each group were infected (Balb/c I, XID I and XID + B1 I), totalizing six groups (n = 5). After 45 days of infection, the ileum was collected for histological processing and analysis. After infection, the XID animals showed an increase in the thickness of the intestinal layers, in the depth and width of the crypt and in the villi width. However, the Balb/c I group showed a reduction in almost all these parameters, whereas the villi width was increased. The villi height decreased in the infected XID animals; however, it was increased in the XID + B1 I group. Leishmania (L) infantum chagasiinfection caused a decrease in the number of Paneth cells; however, their area was increased. Finally, goblet cells and enterocytes presented different change profiles among groups. This study showed that the parasite infection causes structural and histopathological alterations in the intestine. These changes might be influenced by the absence of B‐1 cells.

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 78%

Section: Methodsmentioning

confidence: 99%

“…Therefore, the goblet cells from each animal were counted along the 2560 epithelial cells. Then, the proportion of these cells in relation to 100 epithelial cells was calculated …”

Section: Methodsmentioning

confidence: 99%

Section: Histological Analysesmentioning

confidence: 99%

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