Insights into zinc and cadmium biology in the nematode Caenorhabditis elegans

Dietrich, Nicholas; Tan, Chieh-Hsiang; Cubillas, Ciro; Earley, Brian J.; Kornfeld, Kerry

doi:10.1016/j.abb.2016.05.021

Cited by 26 publications

(32 citation statements)

References 117 publications

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“…Regarding CeHSP17 induction by metals, our data also show that high concentrations of cadmium and zinc, which is clearly cytotoxic in wild-type C. elegans (Dietrich et al, 2016), seemed the most potent inducer of the CeHSP17, which appeared to be highly upregulated by up to three and twofold, respectively (Fig. 4).…”

Section: Discussionmentioning

confidence: 60%

Chemical Signals of Vector Beetle Facilitate the Prevalence of a Native Fungus and the Invasive Pinewood Nematode

Zhang

Lü

et al. 2017

Journal of Nematology

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Small heat shock proteins (sHSP) are ubiquitously found in all organisms, and with other heat shock proteins (HSP) such as HSP60, HSP70, HSP90, HSP100 made up the molecular chaperone family. They are involved in a wide range of biological processes which include among others cell resistance to biological and environmental stress conditions. In this study, we show by western blotting that CeHSP17, an sHSP of Caenorhabiditis elegans, is significantly induced by high temperatures. Furthermore, in response to metal stress, the CeHSP17 protein expression was significantly induced by cadmium and zinc at high concentration of clearly cytotoxic range in wild-type C. elegans. Altogether, our results show the involvement of CeHSP17 protein in both environmental and biological stresses in C. elegans and establish for the first time the expression pattern of the CeHSP17 protein in response to thermal and metal stress conditions in C. elegans. The responses of CeHSP17 protein expression may serve as potential sensitive biomarker for metal-induced toxicity monitoring and environmental risk assessment.

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Section: Discussionmentioning

confidence: 60%

Chemical Signals of Vector Beetle Facilitate the Prevalence of a Native Fungus and the Invasive Pinewood Nematode

Zhang

Lü

et al. 2017

Journal of Nematology

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“…This transcriptional response requires the metal response element (MRE) in gene promoters and the trans- acting Metal regulatory Transcription Factor 1(MTF) ( 14 – 16 ). The nematode Caenorhabditis elegans has been demonstrated to be a useful and relevant model organism to study zinc homeostasis, based on its simple anatomy, powerful genetic techniques and conservation of key genes including metallothioneins, CDF and ZIP ( 17 – 24 ). In C. elegans , high zinc homeostasis also involves transcriptional activation of metallothionein and cdf genes.…”

Section: Introductionmentioning

confidence: 99%

A pathway for low zinc homeostasis that is conserved in animals and acts in parallel to the pathway for high zinc homeostasis

Dietrich

Schneider

Kornfeld

2017

Nucleic Acids Research

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The essential element zinc plays critical roles in biology. High zinc homeostasis mechanisms are beginning to be defined in animals, but low zinc homeostasis is poorly characterized. We investigated low zinc homeostasis in Caenorhabditis elegans because the genome encodes 14 evolutionarily conserved Zrt, Irt-like protein (ZIP) zinc transporter family members. Three C. elegans zipt genes were regulated in zinc-deficient conditions; these promoters contained an evolutionarily conserved motif that we named the low zinc activation (LZA) element that was both necessary and sufficient for activation of transcription in response to zinc deficiency. These results demonstrated that the LZA element is a critical part of the low zinc homeostasis pathway. Transcriptional regulation of the LZA element required the transcription factor ELT-2 and mediator complex member MDT-15. We investigated conservation in mammals by analyzing LZA element function in human cultured cells; the LZA element-mediated transcriptional activation in response to zinc deficiency in cells, suggesting a conserved pathway of low zinc homeostasis. We propose that the pathway for low zinc homeostasis, which includes the LZA element and ZIP transporters, acts in parallel to the pathway for high zinc homeostasis, which includes the HZA element, HIZR-1 transcription factor and cation diffusion facilitator transporters.

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“…For example, transmembrane Zn transporters such as cdf‐proteins may influence Zn homeostasis by mobilizing Zn. Cdf‐2 is involved in the storage of Zn in vesicles of intestinal cells, while cdf‐1 may transport Zn from these cells to the body cavity or intestinal lumen to promote excretion (Davis et al, ; Dietrich, Tan, Cubillas, Earley, & Kornfeld, ). In our earlier study (unpublished data) the potential role of cdf‐2 in mitigating Cd toxicity was identified, and thus it is possible that Cd is also stored in the gut granules, thereby rendering it biologically inactive.…”

Section: Discussionmentioning

confidence: 99%

The interplay between chemical speciation and physiology determines the bioaccumulation and toxicity of Cu(II) and Cd(II) to Caenorhabditis elegans

Moyson

Town

Joosen

et al. 2018

J of Applied Toxicology

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Using the well-documented model organism Caenorhabditis elegans, a combined analysis of metal speciation in the exposure medium and body burdens of metals (Zn, Cu and Cd) was performed, and factors that are predictive of toxicological endpoints in single metal and mixed metal exposures were identified. Cu, and to a lesser extent Cd, is found to associate with Escherichia coli in the exposure medium (the food source for C. elegans) as evidenced by the observed decrease in both their dissolved and free metal ion concentrations. Together with a critical analysis of literature data, our results suggest that free metal ion concentrations and thus aqueous uptake routes are the best predictor of internal concentrations under all conditions considered, and of metal toxicity in single metal exposures. Additional factors are involved in determining the toxicity of metal mixtures. In general, the eventual adverse effects of metals on biota are expected to be a consequence of the interplay between chemical speciation in the exposure medium, timescale of exposure, exposure route as well as the nature and timescale of the biotic handling pathways.

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Insights into zinc and cadmium biology in the nematode Caenorhabditis elegans

Cited by 26 publications

References 117 publications

Chemical Signals of Vector Beetle Facilitate the Prevalence of a Native Fungus and the Invasive Pinewood Nematode

Chemical Signals of Vector Beetle Facilitate the Prevalence of a Native Fungus and the Invasive Pinewood Nematode

A pathway for low zinc homeostasis that is conserved in animals and acts in parallel to the pathway for high zinc homeostasis

The interplay between chemical speciation and physiology determines the bioaccumulation and toxicity of Cu(II) and Cd(II) to Caenorhabditis elegans

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