Insights into the spatial and temporal organisation of plant metabolism from network flux analysis

Moreira, Thiago Batista; Lima, Janderson Moraes; Coca, Guilherme C.; Williams, Thomas Christopher Rhys

doi:10.1007/s40626-018-0132-3

Cited by 11 publications

(8 citation statements)

References 82 publications

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“…Recently, in silico systems approaches have been applied to plant metabolism [10,11], facilitating the development of computational representations of entire metabolic networks from genome sequences. Furthermore, the availability of transcriptomes and whole-genome sequences of algae and earlierdiverging land plants has provided researchers with valuable resources for metabolic modeling [12][13][14].…”

Section: Introductionmentioning

confidence: 99%

Multiple Metabolic Innovations and Losses Are Associated with Major Transitions in Land Plant Evolution

et al. 2020

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Section: Introductionmentioning

confidence: 99%

Multiple Metabolic Innovations and Losses Are Associated with Major Transitions in Land Plant Evolution

et al. 2020

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“…Leaf anatomical structure can respond to environmental changes and influence the above-mentioned parameters, creating intricate and complex changes in metabolic cycles (Moreira et al 2019). For instance, stomatal conductance can change accordingly to stomata number, density and shape and also epidermis area (de Boer et al 2016); mesophyll conductance can be altered by cell organization, intercellular space patterns and leaf thickness (Terashima et al 2011); CO 2 availability to Rubisco can be affected by the same factors as mesophyll conductance and by the physical distribution of carbon assimilation specialized cells (Gago et al 2016); and ATP synthesis through light reactions may be altered, for instance, by chloroplast arrangement (Xiao et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Phenotypic plasticity of leaf anatomical traits helps to explain gas-exchange response to water shortage in grasses of different photosynthetic types

Arantes

Filho

Pennacchi

et al. 2020

Theor. Exp. Plant Physiol.

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C3 and C4 plants, as their intermediates, respond differently to short-term changes in environmental conditions. This difference is linked to contrasting levels of phenotypic plasticity and photosynthetic apparatus specialization. Phenotypic plasticity is an underexplored topic although its understanding is crucial to predict plant behaviour in future climatic scenarios. In this research, the phenotypic plasticity of anatomical traits and its influence to carbon uptake efficiency was studied in plants with different photosynthetic types, under contrasting water regimes. Oryza sativa cvs. Soberana (droughtsensitive) and Douradão (drought-tolerant) (C3), Homolepis isocalycia (C3 proto-Kranz) and Andropogon gayanus (C4), grown at three water treatments (100, 75 and 50% of substrate water holding capacity), were phenotyped for leaf anatomy and gas-exchange parameters. The results showed that plasticity trends indicated different strategies between O. sativa cultivars to deal with water shortage, explaining their classification as drought-sensitive or tolerant. We also mapped typical characteristics of C3-C4 intermediate plant, H. isocalycia, mainly in the ratio mesophyll:bundle sheath cells and hypothesize how it may influence photosynthesis. Finally, we have confirmed previous claims that C4 carbon uptake advantages may be limited under severe drought conditions, as A. gayanus have drastically reduced its photosynthetic rates at lower water levels. By studying C3-C4 intermediates, this study may also be a starting point to unravel the trade-offs of anatomical changes during the evolutionary process from C3 to C4 photosynthesis, and also improve the understanding of their impact in carbon uptake in different water conditions.

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“…This approach simplifies the mathematical representation of the system by considering only the stoichiometry of the metabolic reactions and uses experimental constraints and an optimization objective to make predictions about flux distributions in the metabolic network at steady state (Nikoloski et al, 2015). This approach has been proven capable of making quantitatively realistic predictions of plant metabolic behavior (Basler et al, 2018;Moreira et al, 2019). A number of studies have applied FBA to C 3 leaf metabolism (de Oliveira Dal'Molin et al, 2010Poolman et al, 2013Poolman et al, , 2014Arnold and Nikoloski, 2014;Cheung et al, 2014Cheung et al, , 2015Lakshmanan et al, 2015), but the specific questions of the role of mitochondria in the light and the energetic coupling between subcellular compartments have not been explicitly considered.…”

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confidence: 99%

Leaf Energy Balance Requires Mitochondrial Respiration and Export of Chloroplast NADPH in the Light

2019

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Key aspects of leaf mitochondrial metabolism in the light remain unresolved. For example, there is debate about the relative importance of exporting reducing equivalents from mitochondria for the peroxisomal steps of photorespiration versus oxidation of NADH to generate ATP by oxidative phosphorylation. Here, we address this and explore energetic coupling between organelles in the light using a diel flux balance analysis model. The model included more than 600 reactions of central metabolism with full stoichiometric accounting of energy production and consumption. Different scenarios of energy availability (light intensity) and demand (source leaf versus a growing leaf) were considered, and the model was constrained by the nonlinear relationship between light and CO 2 assimilation rate. The analysis demonstrated that the chloroplast can theoretically generate sufficient ATP to satisfy the energy requirements of the rest of the cell in addition to its own. However, this requires unrealistic high light use efficiency and, in practice, the availability of chloroplast-derived ATP is limited by chloroplast energy dissipation systems, such as nonphotochemical quenching, and the capacity of the chloroplast ATP export shuttles. Given these limitations, substantial mitochondrial ATP synthesis is required to fulfill cytosolic ATP requirements, with only minimal, or zero, export of mitochondrial reducing equivalents. The analysis also revealed the importance of exporting reducing equivalents from chloroplasts to sustain photorespiration. Hence, the chloroplast malate valve and triose phosphate-3-phosphoglycerate shuttle are predicted to have important metabolic roles, in addition to their more commonly discussed contribution to the avoidance of photooxidative stress.

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Insights into the spatial and temporal organisation of plant metabolism from network flux analysis

Cited by 11 publications

References 82 publications

Multiple Metabolic Innovations and Losses Are Associated with Major Transitions in Land Plant Evolution

Multiple Metabolic Innovations and Losses Are Associated with Major Transitions in Land Plant Evolution

Phenotypic plasticity of leaf anatomical traits helps to explain gas-exchange response to water shortage in grasses of different photosynthetic types

Leaf Energy Balance Requires Mitochondrial Respiration and Export of Chloroplast NADPH in the Light

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