Insights into the role of circular RNA in macrophage activation and fibrosis disease

Xu, Mengxue; Xie, Feiting; Tang, Xinyi; Wang, Tingting; Wang, Shengjun

doi:10.1016/j.phrs.2020.104777

Cited by 25 publications

(15 citation statements)

References 138 publications

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“…Recently, circRNAs have been proven to be differentially expressed in various diseases, especially cancers, due to their stability, abundance, conservation, and spatiotemporal specificity [22][23][24][25]. Currently, many studies have detected the differential expression of circRNAs in GC tissues [26][27][28].…”

Section: Discussionmentioning

confidence: 99%

circCUL2 regulates gastric cancer malignant transformation and cisplatin resistance by modulating autophagy activation via miR-142-3p/ROCK2

et al. 2020

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Background Circular RNAs (circRNAs) are a class of noncoding RNAs (ncRNAs) and can modulate gene expression by binding to miRNAs; further, circRNAs have been shown to participate in several pathological processes. However, the expression and biological function of circCUL2 in gastric cancer (GC) remains largely unknown. Methods circRNA microarrays and quantitative real-time PCR (qRT-PCR) were used to identify differentially expressed circRNAs in GC tissues and cell lines. circCUL2 knockdown and overexpression were performed to indicate the functional role of circCUL2 in vitro and in vivo. The expression and regulation of circCUL2, miR-142-3p and ROCK2 were evaluated using fluorescence in situ hybridization (FISH), dual-luciferase assays, RNA pull-down assays, RNA immunoprecipitation (RIP) and rescue experiments. Furthermore, the regulation of cisplatin sensitivity and autophagy by circCUL2/miR-142-3p/ROCK2 was demonstrated by cellular apoptosis assays, western blot, immunofluorescence and transmission electron microscopy analyses. Results The level of circCUL2, which is stable and cytoplasmically localized, was significantly reduced in GC tissues and cells. Overexpressed circCUL2 inhibited malignant transformation in vitro and tumorigenicity in vivo. In the AGS and SGC-7901 cell lines, circCUL2 sponged miR-142-3p to regulate ROCK2, thus modulating tumor progression. Furthermore, in the AGS/DDP and SGC-7901/DDP cell lines, circCUL2 regulated cisplatin sensitivity through miR-142-3p/ROCK2-mediated autophagy activation. Conclusion circCUL2 may function as a tumor suppressor and regulator of cisplatin sensitivity through miR-142-3p/ROCK2-mediated autophagy activation, which could be a key mechanism and therapeutic target for GC.

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Section: Discussionmentioning

confidence: 99%

circCUL2 regulates gastric cancer malignant transformation and cisplatin resistance by modulating autophagy activation via miR-142-3p/ROCK2

et al. 2020

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“…Accumulating evidence has suggested that circRNAs play crucial roles in the immune response and its regulation (Fig. 3 ) (Chen et al, 2017b , 2019c ; Xu et al, 2018b , 2020a ; Yang et al, 2018 ; Zhou et al, 2019b ; Awan et al, 2020 ). First, circRNAs are important regulators of blood cell biogenesis, differentiation and activation (Chen et al, 2019c ; Zhou et al, 2019b ; Xu et al, 2020a ).…”

Section: Blood Circrnas and Liquid Biposy Biomarkersmentioning

confidence: 99%

“…3 ) (Chen et al, 2017b , 2019c ; Xu et al, 2018b , 2020a ; Yang et al, 2018 ; Zhou et al, 2019b ; Awan et al, 2020 ). First, circRNAs are important regulators of blood cell biogenesis, differentiation and activation (Chen et al, 2019c ; Zhou et al, 2019b ; Xu et al, 2020a ). In a comprehensive study of circRNA expression in hematopoietic progenitors, differentiated lymphoid and myeloid cells, Nicolet et al observed a cell-type specific pattern of circRNA expression profiles in blood cells, and the type and number of circRNAs increased upon hematopoietic maturation (Nicolet et al, 2018 ).…”

Section: Blood Circrnas and Liquid Biposy Biomarkersmentioning

confidence: 99%

The potential of using blood circular RNA as liquid biopsy biomarker for human diseases

2020

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Circular RNA (circRNA) is a novel class of single-stranded RNAs with a closed loop structure. The majority of circRNAs are formed by a back-splicing process in pre-mRNA splicing. Their expression is dynamically regulated and shows spatiotemporal patterns among cell types, tissues and developmental stages. CircRNAs have important biological functions in many physiological processes, and their aberrant expression is implicated in many human diseases. Due to their high stability, circRNAs are becoming promising biomarkers in many human diseases, such as cardiovascular diseases, autoimmune diseases and human cancers. In this review, we focus on the translational potential of using human blood circRNAs as liquid biopsy biomarkers for human diseases. We highlight their abundant expression, essential biological functions and significant correlations to human diseases in various components of peripheral blood, including whole blood, blood cells and extracellular vesicles. In addition, we summarize the current knowledge of blood circRNA biomarkers for disease diagnosis or prognosis.

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“…In the cytoplasm circRNAs are implicated in RNA regulation, or miRNA regulation by acting as competing endogenous RNA [40]; moreover, circRNAs may interact with proteins acting as bridges for protein-protein interaction. In the nucleus, these control gene expressions have been found to be involved in several physiological and pathological process [41][42][43].…”

Section: Non-coding Rnasmentioning

confidence: 99%

Hypoxia-Induced Non-Coding RNAs Controlling Cell Viability in Cancer

Barreca

Zichittella

Alessandro

et al. 2021

IJMS

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Hypoxia, a characteristic of the tumour microenvironment, plays a crucial role in cancer progression and therapeutic response. The hypoxia-inducible factors (HIF-1α, HIF-2α, and HIF-3α), are the master regulators in response to low oxygen partial pressure, modulating hypoxic gene expression and signalling transduction pathways. HIFs’ activation is sufficient to change the cell phenotype at multiple levels, by modulating several biological activities from metabolism to the cell cycle and providing the cell with new characteristics that make it more aggressive. In the past few decades, growing numbers of studies have revealed the importance of non-coding RNAs (ncRNAs) as molecular mediators in the establishment of hypoxic response, playing important roles in regulating hypoxic gene expression at the transcriptional, post-transcriptional, translational, and posttranslational levels. Here, we review recent findings on the different roles of hypoxia-induced ncRNAs in cancer focusing on the data that revealed their involvement in tumour growth.

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Insights into the role of circular RNA in macrophage activation and fibrosis disease

Cited by 25 publications

References 138 publications

circCUL2 regulates gastric cancer malignant transformation and cisplatin resistance by modulating autophagy activation via miR-142-3p/ROCK2

circCUL2 regulates gastric cancer malignant transformation and cisplatin resistance by modulating autophagy activation via miR-142-3p/ROCK2

The potential of using blood circular RNA as liquid biopsy biomarker for human diseases

Hypoxia-Induced Non-Coding RNAs Controlling Cell Viability in Cancer

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