Insights into the role of immunosenescence during varicella zoster virus infection (shingles) in the aging cell model

Kim, JiAe; Park, Seulki; Kumar, Mukesh; Lee, Chanhee; Shin, Ok Sarah

doi:10.1016/j.jdermsci.2017.02.210

Cited by 4 publications

(5 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, due to a recent outbreak, a growing number of cases were reported from countries where the disease is not endemic [4][5][6] . The genomic monitoring of the 2022 MPXV outbreak has demonstrated that the recently circulating MPXV strain is probably related to the less likely pathogenic West African clade of MPXVs and forms a highly divergent new clade with an elevated mutation rate [7][8][9] .…”

Section: Background and Summarymentioning

confidence: 99%

In-depth Temporal Transcriptome Profiling of Monkeypox and Host Cells using Nanopore Sequencing

Kakuk¹,

Dörmő²,

Csabai³

et al. 2022

Preprint

View full text Add to dashboard Cite

The recent Monkeypox outbreak showed the importance of studying the basic biology of orthopoxviruses. However, the transcriptome of its causative agent has not been investigated before neither with short-, nor with long-read sequencing approaches. This Oxford Nanopore long-read RNA-Sequencing dataset fills this gap. Our direct cDNA and native RNA sequencing data enable the in-depth characterization of the transcriptomic architecture and dynamics of the gene expressions of monkeypox virus; and also the deeper understanding of the changes it causes in the host cells on a transcriptome level.

show abstract

Section: Background and Summarymentioning

confidence: 99%

In-depth Temporal Transcriptome Profiling of Monkeypox and Host Cells using Nanopore Sequencing

Kakuk¹,

Dörmő²,

Csabai³

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…Previously, Jones et al (2014) showed that the differentiation status of keratinocytes affects the replication pattern of the viral genome and protein expression using dual RNA-seq in VZV-infected keratinocytes. In line with this, our previous study using senescent HDFs and progeria cells highlighted the effect of cellular replicative senescence on VZV replication (Kim et al, 2015(Kim et al, , 2016. Here, we aimed to evaluate the underlying features of dermal cell type-specific and postinfection time-specific mRNA profiles using high-throughput dual RNA-seq.…”

Section: Discussionmentioning

confidence: 81%

“…Our previous RNA-seq analysis study suggested that VZV infection in HDFs results in distinct up-regulation of the antiviral immune response (Kim et al, 2015). To obtain global and dynamic gene expression profiles of the VZV-induced immune response, HDFs and HaCaTs were infected with mock or VZV for 24, 48, and 72 hours, and the dual host/virus transcriptome was analyzed by RNA-seq.…”

Section: Rig-i Is Not Essential For Vzv Replication Controlmentioning

confidence: 99%

See 1 more Smart Citation

STING Is Involved in Antiviral Immune Response against VZV Infection via the Induction of Type I and III IFN Pathways

Kim

Park

Seo

et al. 2017

Journal of Investigative Dermatology

Self Cite

View full text Add to dashboard Cite

Varicella zoster virus (VZV) is a human-restricted α-herpesvirus that exhibits tropism for the skin. The VZV host receptors and downstream signaling pathways responsible for the antiviral innate immune response in the skin are not completely understood. Here, we show that STING mediates an important host defense against VZV infection in dermal cells including human dermal fibroblasts and HaCaT keratinocytes. Inhibition of STING using small interfering-RNA or short hairpin RNA-mediated gene disruption resulted in enhanced viral replication but diminished IRF3 phosphorylation and induction of IFNs and proinflammatory cytokines. Pretreatment with STING agonists resulted in reduced VZV glycoprotein E expression and viral replication. Additionally, using RNA sequencing to analyze dual host and VZV transcriptomes, we identified several host immune genes significantly induced by VZV infection. Furthermore, significant up-regulation of IFN-λ secretion was observed after VZV infection, partly through a STING-dependent pathway; IFN-λ was shown to be crucial for antiviral defense against VZV in human dermal cells. In conclusion, our data provide an important insight into STING-mediated induction of type I and III IFNs and subsequent antiviral signaling pathways that regulate VZV replication in human dermal cells.

show abstract

“…Since cell plating and culture are relatively easy and standardized, various cell types are studied, including stable cell lines, primary human cells, stem cells, and immune cells [ [30] , [31] , [32] , [33] , [34] , [35] , [36] , [37] , [38] ]. The platform allows the real-time observation of cellular behaviors, such as viral entry into cells, changes in metabolic activity of cells, activation of immune-related factors, and morphological changes [ [39] , [40] , [41] , [42] , [43] , [44] , [45] , [46] , [47] , [48] , [49] ]. In addition, cells were easily harvested and analyzed for biomolecular changes, including protein and mRNA levels.…”

Section: Conventional Models For Studying Infectious Viral Diseases: ...mentioning

confidence: 99%

3D engineered tissue models for studying human-specific infectious viral diseases

Hwang

Seo²,

Choi³

et al. 2023

Bioactive Materials

View full text Add to dashboard Cite

Insights into the role of immunosenescence during varicella zoster virus infection (shingles) in the aging cell model

Cited by 4 publications

References 25 publications

In-depth Temporal Transcriptome Profiling of Monkeypox and Host Cells using Nanopore Sequencing

In-depth Temporal Transcriptome Profiling of Monkeypox and Host Cells using Nanopore Sequencing

STING Is Involved in Antiviral Immune Response against VZV Infection via the Induction of Type I and III IFN Pathways

3D engineered tissue models for studying human-specific infectious viral diseases

Contact Info

Product

Resources

About