Insights Into the Role of Collagen in Vocal Fold Health and Disease

Tang, Sharon; Mohad, Vidisha; Gowda, Madhu; Thibeault, Susan L.

doi:10.1016/j.jvoice.2017.01.008

Cited by 27 publications

(22 citation statements)

References 48 publications

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“…Matrix metalloproteinase (MMP) 1 is responsible for the degradation of collagen type 1 and 3. Tissue inhibitor of metalloproteinase (TIMP) on the contrary is considered to be the antagonist of MMP [ 37 , 38 ].…”

Section: Methodsmentioning

confidence: 99%

In vitro mechanical vibration down-regulates pro-inflammatory and pro-fibrotic signaling in human vocal fold fibroblasts

et al. 2020

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Introduction Voice rest following phonotrauma or phonosurgery has a considerable clinical impact, but clinical recommendations are inconsistent due to inconclusive data. As biopsies of the vocal folds (VF) for molecular biology studies in humans are unethical, we established a new in vitro model to explore the effects of vibration on human vocal fold fibroblasts (hVFF) in an inflammatory and normal state, which is based on previously published models. Methods By using a phonomimetic bioreactor we were able to apply predefined vibrational stress patterns on hVFF cultured under inflammatory or normal conditions. Inflammatory and pro-fibrotic stimuli were induced by interleukin (IL)1β and transforming growth factor (TGF)β1, respectively. Mechanical stimulation was applied four hours daily, over a period of 72 hours. Outcome measurements comprised assessment of extracellular matrix (ECM)-related components, angiogenic factors, and inflammatory and fibrogenic markers on gene expression and protein levels. Results Under inflammatory conditions, the inflammatory cytokine IL11, as well as the myofibroblast marker alpha smooth muscle actin (α-SMA) were significantly reduced when additional vibration was applied. The desirable anti-fibrotic ECM component hyaluronic acid was increased following cytokine treatment, but was not diminished following vibration. Conclusion Our experiments revealed the effect of vibrational stress on hVFF in an inflammatory state. Elevated levels of certain pro-inflammatory/pro-fibrotic factors could be mitigated by additional vibrational excitation in an in vitro setting. These findings corroborate clinical studies which recommend early voice activation following an acute event.

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Section: Methodsmentioning

confidence: 99%

In vitro mechanical vibration down-regulates pro-inflammatory and pro-fibrotic signaling in human vocal fold fibroblasts

et al. 2020

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“… Skin-collagen-molecular structure. Adapted with permissions from: (1) Tang et al, Journal of Voice, published by Elsevier Inc. (Amsterdam, The Netherlands), 2017; (2) Reilly and Lozano, Plastic and Aesthetic Research, published by OAE Publishing Inc., 2021 (©2021 MINERVA Research Labs Ltd. (106 New Bond St. London, UK) all rights reserved) [ 29 , 30 ]. …”

Section: Figurementioning

confidence: 99%

Collagen Extraction from Animal Skin

Matinong

Chisti

Pickering

et al. 2022

Biology

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Collagen is the most abundant structural protein in animals. It is the major component of skin. It finds uses in cosmetics, medicine, yarn production and packaging. This paper reviews the extraction of collagen from hides of most consumed animals for meat with the focus on literature published since 2000. The different pretreatment and extraction techniques that have been investigated for producing collagen from animal skins are reviewed. Pretreatment by enzymatic, acid or alkaline methods have been used. Extraction by chemical hydrolysis, salt solubilization, enzymatic hydrolysis, ultrasound assisted extraction and other methods are described. Post-extraction purification methods are also explained. This compilation will be useful for anyone wishing to use collagen as a resource and wanting to further improve the extraction and purification methods.

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“…The complex tissue consists of superficial and deep layers separated by the lamina propria [ 12 ]. Additionally, the lamina propria is further divided into three parts: (1) superficial, (2) intermediate and (3) deep layers [ 13 ]. The myoelastic-aerodynamic theory explains the unique characteristics of the vocal fold and the neuromuscular control that permits phonation [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…Elastin enables vocal fold tissue to possess elastic properties and is more abundant in the superficial parts of the lamina propria than its deeper layers [ 18 ]. On the other hand, collagen in the vocal fold functions as a mechanical support to withstand high-frequency vibration and stretching [ 13 ]. The abundance of collagen was found in the deep layer of the lamina propria.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Evaluation of Biomaterials for Vocal Fold Injection: A Systematic Review

Baki

Lokanathan

et al. 2021

Polymers

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Vocal fold injection is a preferred treatment in glottic insufficiency because it is relatively quick and cost-saving. However, researchers have yet to discover the ideal biomaterial with properties suitable for human vocal fold application. The current systematic review employing PRISMA guidelines summarizes and discusses the available evidence related to outcome measures used to characterize novel biomaterials in the development phase. The literature search of related articles published within January 2010 to March 2021 was conducted using Scopus, Web of Science (WoS), Google Scholar and PubMed databases. The search identified 6240 potentially relevant records, which were screened and appraised to include 15 relevant articles based on the inclusion and exclusion criteria. The current study highlights that the characterization methods were inconsistent throughout the different studies. While rheologic outcome measures (viscosity, elasticity and shear) were most widely utilized, there appear to be no target or reference values. Outcome measures such as cellular response and biodegradation should be prioritized as they could mitigate the clinical drawbacks of currently available biomaterials. The review suggests future studies to prioritize characterization of the viscoelasticity (to improve voice outcomes), inflammatory response (to reduce side effects) and biodegradation (to improve longevity) profiles of newly developed biomaterials.

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Insights Into the Role of Collagen in Vocal Fold Health and Disease

Cited by 27 publications

References 48 publications

In vitro mechanical vibration down-regulates pro-inflammatory and pro-fibrotic signaling in human vocal fold fibroblasts

In vitro mechanical vibration down-regulates pro-inflammatory and pro-fibrotic signaling in human vocal fold fibroblasts

Collagen Extraction from Animal Skin

In Vitro Evaluation of Biomaterials for Vocal Fold Injection: A Systematic Review

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