Insights into the metabolite profiles of Rubus chingii Hu at different developmental stages of fruit

Abstract

The fruits of Rubus chingii Hu have high medicinal and nutritional values. However, the metabolite profiles of R. chingii, especially the alterations during different development stages of fruit, have not been comprehensively analyzed, hindering the effective utilization of the unique species. In this study, we comprehensively analyzed the metabolites of R. chingii fruit at four developmental stages using systematic untargeted and targeted liquid chromatography-mass spectrometry metabolomics analysis and identified 682 metabolites. Significant changes were observed in metabolite accumulation and composition in fruits during the different developmental stages. The contents of the index components, kaempferol-3-O-rutinoside and ellagic acid, were the highest in immature fruit. The analysis identified 64 differentially expressed flavonoids and 39 differentially expressed phenolic acids; the accumulation of most of these differentially expressed metabolites decreased with the developmental stages of fruit from immaturity to maturity. These results confirmed that the developmental stages of fruit are a critical factor in determining its secondary metabolite compositions.This study elucidated the metabolic profile of R. chingii fruit at different stages of development to understand the dynamic changes in metabolites.

Identification and Functional Verification of the Glycosyltransferase Gene Family Involved in Flavonoid Synthesis in Rubus chingii Hu

“…In addition, R. chingii can also be used in medicine, and it is the only species of Chinese raspberry listed in the Chinese Pharmacopoeia [3]. Its medicinal part is its immature dry fruit, which contains a large number of flavonoids, terpenoids, polysaccharides, and other substances, especially flavonoids [4,5], which can play a strong role in anti-oxidation, anti-inflammation, anticancer, anti-hypertension and the protection of blood vessels [3,6,7]. Therefore, raspberry is a kind of medicine and a food homologous variety, which has great prospects in research and development.…”

Metabolomics analysis reveals characteristic metabolites in different levels of oxaliplatin‐induced neurotoxicity

“…Untargeted metabolomics has achieved extensive substance coverage, yielding comprehensive metabolite information, and detecting many previously unknown components. Currently, metabolomics has found applications in various fields, including biomarkers [12][13][14], disease diagnosis [15][16][17], drug safety [18], toxicology research [19], and plant studies [20][21]. Furthermore, Metabolomics also offers the potentiality of detecting, identifying, and quantifying variations in endogenous metabolites reflecting the balance of specific metabolic pathways, and biological changes of the internal microenvironment [22,23].…”