Insights into the Lignocellulose-Degrading Enzyme System of
            <i>Humicola grisea</i>
            var.
            <i>thermoidea</i>
            Based on Genome and Transcriptome Analysis

Steindorff, Andrei Stecca; Serra, Luana Assis; Formighieri, Eduardo Fernandes; Faria, Fabrícia Paula de; Poças-Fonseca, Marcio José; Almeida, J. R. M. de

doi:10.1128/spectrum.01088-21

Cited by 15 publications

(9 citation statements)

References 58 publications

(84 reference statements)

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“…The following supporting information can be downloaded at: , Figure S1: The variations in TN removal efficiency (%) in different SPD systems; Table S1: Primers of target genes used in qPCR analysis; Table S2: The results of ANOVA for the model (response: TN removal efficiency); Table S3: The optimized TN removal efficiency based on RSM; Table S4: The actual TN removal efficiency under optimized conditions; Table S5: Functional classification of major genera in bacterial and fungal communities. [ 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 , 72 , 73 , 74 , 75 , 76 , 77 , 78 , 79 , 80 , 81 , 82 , 83 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 ] of the references in the main text are from the supplementary material.…”

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confidence: 99%

Improved Denitrification Performance of Polybutylene Succinate/Corncob Composite Carbon Source by Proper Pretreatment: Performance, Functional Genes and Microbial Community Structure

et al. 2023

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Blending biodegradable polymers with plant materials is an effective method to improve the biodegradability of solid carbon sources and save denitrification costs, but the recalcitrant lignin in plant materials hinders the microbial decomposition of available carbon sources. In the present study, corncob pretreated by different methods was used to prepare polybutylene succinate/corncob (PBS/corncob) composites for biological denitrification. The PBS/corncob composite with alkaline pretreatment achieved the optimal NO3−-N removal rate (0.13 kg NO3−-N m−3 day−1) with less adverse effects. The pretreatment degree, temperature, and their interaction distinctly impacted the nitrogen removal performance and dissolved organic carbon (DOC) release, while the N2O emission was mainly affected by the temperature and the interaction of temperature and pretreatment degree. Microbial community analysis showed that the bacterial community was responsible for both denitrification and lignocellulose degradation, while the fungal community was primarily in charge of lignocellulose degradation. The outcomes of this study provide an effective strategy for improving the denitrification performance of composite carbon sources.

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confidence: 99%

Improved Denitrification Performance of Polybutylene Succinate/Corncob Composite Carbon Source by Proper Pretreatment: Performance, Functional Genes and Microbial Community Structure

et al. 2023

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“…Under compost treatment, the relative abundance of Humicola significantly increased at S1, S2 and S3, while the relative abundance of Chaetomium significantly increased at S1, indicating compost promoted Humicola and Chaetomium population growth. Humicola are filamentous fungi found in the soil that are capable of producing enzymes that act synergistically for the degradation of lignocellulosic biomass which leads to the decomposition of residues [ 61 ]. Chaetomium are largely used to control and limit soilborne pathogens, thereby reducing pathogenic fungi and promoting barley plant growth [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

Date Palm Waste Compost Application Increases Soil Microbial Community Diversity in a Cropping Barley (Hordeum vulgare L.) Field

Ghouili

Abid

Hogue

et al. 2023

Biology

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Application of date palm waste compost is quite beneficial in improving soil properties and crop growth. However, the effect of its application on soil microbial communities is less understood. High-throughput sequencing and quantitative real-time PCR (qPCR) were used to evaluate the effect of compost application on the soil microbial composition in a barley field during the tillering, booting and ripening stages. The results showed that compost treatment had the highest bacterial and fungal abundance, and its application significantly altered the richness (Chao1 index) and α-diversity (Shannon index) of fungal and bacterial communities. The dominant bacterial phyla found in the samples were Proteobacteria and Actinobacteria while the dominant fungal orders were Ascomycota and Mortierellomycota. Interestingly, compost enriched the relative abundance of beneficial microorganisms such as Chaetomium, Actinobacteriota, Talaromyces and Mortierella and reduced those of harmful microorganisms such as Alternaria, Aspergillus and Neocosmospora. Functional prediction based on Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) showed that amplicon sequence variant (ASV) sequences related to energy metabolism, amino acid metabolism and carbohydrate metabolism were associated with compost-treated soil. Based on Fungi Functional Guild (FUNGuild), identified fungi community metabolic functions such as wood saprotroph, pathotroph, symbiotroph and endophyte were associated with compost-treated soil. Overall, compost addition could be considered as a sustainable practice for establishing a healthy soil microbiome and subsequently improving the soil quality and barley crop production.

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“…thermoidea is an aerobic and thermophilic fungus with optimal growth at 40–42 °C. This strain was originally isolated from composting material 14 and classified according to its ability to produce enzymes, mainly endoxylanases, at high temperatures 15 , 16 . When grown in sugarcane bagasse, this fungus secretes a GH11 xylanase known as HXYN2, with 227 amino acids, a molecular mass of 23 kDa, pI of 6.1, and optimal temperature and pH of 65 °C and 5.5, respectively 17 .…”

Section: Introductionmentioning

confidence: 99%

Structural and biochemical analysis reveals how ferulic acid improves catalytic efficiency of Humicola grisea xylanase

Oliveira

Garay

Souza

et al. 2022

Sci Rep

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Humicolagrisea var. thermoidea is an aerobic and thermophilic fungus that secretes the GH11 xylanase HXYN2 in the presence of sugarcane bagasse. In this study, HXYN2 was expressed in Pichiapastoris and characterized biochemically and structurally in the presence of beechwood xylan substrate and ferulic acid (FA). HXYN2 is a thermally stable protein, as indicated by circular dichroism, with greater activity in the range of 40–50 °C and pH 5.0–9.0, with optimal temperature and pH of 50 °C and 6.0, respectively. FA resulted in a 75% increase in enzyme activity and a 2.5-fold increase in catalytic velocity, catalytic efficiency, and catalytic rate constant (kcat), with no alteration in enzyme affinity for the substrate. Fluorescence quenching indicated that FA forms a complex with HXYN2 interacting with solvent-exposed tryptophan residues. The binding constants ranged from moderate (pH 7.0 and 9.0) to strong (pH 4.0) affinity. Isothermal titration calorimetry, structural models and molecular docking suggested that hydrogen bonds and hydrophobic interactions occur in the aglycone region inducing conformational changes in the active site driven by initial and final enthalpy- and entropy processes, respectively. These results indicate a potential for biotechnological application for HXYN2, such as in the bioconversion of plant residues rich in ferulic acid.

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Insights into the Lignocellulose-Degrading Enzyme System of Humicola grisea var. thermoidea Based on Genome and Transcriptome Analysis

Cited by 15 publications

References 58 publications

Improved Denitrification Performance of Polybutylene Succinate/Corncob Composite Carbon Source by Proper Pretreatment: Performance, Functional Genes and Microbial Community Structure

Improved Denitrification Performance of Polybutylene Succinate/Corncob Composite Carbon Source by Proper Pretreatment: Performance, Functional Genes and Microbial Community Structure

Date Palm Waste Compost Application Increases Soil Microbial Community Diversity in a Cropping Barley (Hordeum vulgare L.) Field

Structural and biochemical analysis reveals how ferulic acid improves catalytic efficiency of Humicola grisea xylanase

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