Insights into the hippocampus proteome and phosphorylation modification alterations in C57BL/6 revealed the memory improvement mechanisms of a walnut-derived peptide

Walnut-Derived Peptide Improves Cognitive Impairment in Colitis Mice Induced by Dextran Sodium Sulfate via the Microbiota–Gut–Brain Axis (MGBA)

“…23 Moreover, our previous studies have demonstrated that the walnut-derived peptides TW-7 and YVLLPSPK could significantly reduce the escape latency and increase the residence time in the target area in the scopolamine-induced and D-galactose-induced cognitive-impaired mice, respectively. 16,24 Our work established that LP-5 treatment greatly enhanced the learning and memory capacities of DSS-induced IBD mice, which is consistent with previous findings.…”

Improvement of Spatial Memory and Cognitive Function in Mice via the Intervention of Milk Fat Globule Membrane

“…This study used proteomic techniques to determine how MFGM improves mouse cognition and spatial memory. At p < 0.05, proteins with >1.2 fold−change (FC) values were considered as significantly upregulated, while proteins with <0.83 FC values were considered as significantly downregulated [ 20 ]. The expression of 7067 proteins was identified from the protein database.…”

“…Mice were stripped of their brains on ice immediately after losing consciousness, and the left hippocampus was fixed with 4% paraformaldehyde for subsequent staining analysis. The method of H&E staining was referred to in the previously published literature by Wang et al and Geng et al [ 20 , 21 ]. In brief, the fixed left hippocampus was trimmed, dehydrated in gradient alcohol, xylene transparency, and then embedded in paraffin.…”

Insights into the Hippocampus Proteomics Reveal Epigenetic Properties of Walnut-Derived Peptides in a Low-Grade Neuroinflammation Model

“…The technological advance in proteomics analyses is increasingly being used to identify new biomarkers for the detection of neurodegenerative disorders in the hippocampus or cerebrospinal fluid (CSF) or blood-based samples derived from patients and animal models . The emergence of advanced LC–MS/MS platforms has enabled the production of protein mass spectra at specific times and in physiological or pathological states . One of the important characteristics of the scopolamine model of AD is neuroinflammation, which may result from or contribute to the formation of oxidative stress .…”

“…16 The emergence of advanced LC−MS/MS platforms has enabled the production of protein mass spectra at specific times and in physiological or pathological states. 17 One of the important characteristics of the scopolamine model of AD is neuroinflammation, which may result from or contribute to the formation of oxidative stress. 18 The reported studies revealed by Amirahmadi et al that pro-inflammatory cytokines TNF-α, IL-1β, and IL-6, which have been associated with significant neuropathological alterations in neurodegenerative disorders, were much more expressed when scopolamine was administered.…”