Insights into the dual nature of αB-crystallin chaperone activity from the p.P39L mutant at the N-terminal region
Anis Barati,
Leila Rezaei Somee,
Mohammad Bagher Shahsavani
et al.
Abstract:The substitution of leucine to proline at position 39 (p.P39L) in human αB-crystallin (αB-Cry) has been associated with conflicting interpretations of pathogenicity in cataracts and cardiomyopathy. This study aimed to investigate the effects of the p.P39L mutation on the structural and functional features of human αB-Cry. The mutant protein was expressed in Escherichia coli (E. coli) and purified using anion exchange chromatography. We employed a wide range of spectroscopic analyses, gel electrophoresis, trans… Show more
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