Insights into the drug screening approaches in leishmaniasis

Design, synthesis, and biological evaluation of 3,3′-diindolylmethane N-linked glycoconjugate as a leishmanial topoisomerase IB inhibitor with reduced cytotoxicity

“…Reduction of MTT by actively dividing cells constitutes a suitable parameter to assess the viability in an extensive range of cell lines. 25 The effect of bisindoles on MTT reduction was assessed to evaluate their cytotoxicity. The cytotoxicity of potent non-glycosylated DIM ( 2d , 3d , and 4a ) and glycosylated DIM 13 was assessed in the J774A.1 macrophage cell line in terms of drug concentration required to reduce cell toxicity.…”

“…The effect of synthesized 3,3′-DIM derivatives on the viability of L. donovani DD8 cells was determined by the Alamar Blue cell viability assay. 25 Exponentially growing promastigote cells were collected and transferred into a 96-well plate (5 × 10 6 cells per well). 1 mM stock solution of each compound was prepared in DMSO.…”

“…The cytotoxicity of potent compounds was determined against the J774A.1 cell line by the MTT assay. 25,26 J774A.1 is a murine macrophage cell line derived from a tumor in female BALB/c mice. 27 Briefly, J774A.1 cells (2 × 10 4 cells per well) were cultured in DMEM complete medium, plated into a 96-well tissue culture plate and left overnight to adhere.…”