Abstract:The catalytically competent Co(II)-loaded form of the chlorothalonil dehalogenase from Pseudomonas sp. CTN-3 (Chd, EC 3.8.1.2) was characterized by kinetic and spectroscopic methods. Maximum chlorothalonil (TPN; 2,4,5,6-tetrachloroisophtalonitrile) dehalogenase activity was observed in the presence of one Co(II) ion per monomer with kcat and Km values of 12 ± 3 s−1 and 130 ± 10 μM, respectively, providing a catalytic efficiency (kcat/Km) of ∼9.2 × 104 M−1s−1. The dissociation constant (Kd) for Co(II) was deter… Show more
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