Insights into the binding sites of sulforaphane on insulin studied by electrospray ionization mass spectrometry

Abstract

The interaction of SFN was studied with insulin using ESI-MS. SFN is found to bind covalently with the free NH2 group of the N-terminal of the A and B chains of insulin. However, when insulin is reduced SFN preferably binds to SH groups of cysteines. Hence, the present study helps in the understanding of the binding sites of SFN on insulin.

Isolation and characterization of phthalates from Brevibacterium mcbrellneri that cause cytotoxicity and cell cycle arrest

“…Exactive Orbitrap mass spectrometer (Thermo Scientific, Waltham, MA, USA) was used to record electrospray ionization high resolution mass spectra, in positive ion mode (Nagaveni and Prabhakar, 2015[24]). Spray voltage was maintained as 4000 V and capillary voltage as 30 V at 250 °C.…”

Synthesis and Anti‐Inflammatory Activity of 2‐Amino‐4,5,6,7‐tetrahydrobenzo[b]thiophene‐Derived NRF2 Activators

“…In cruciferous vegetables like broccoli, sulforaphane is a classical NRF2 activator that inhibits KEAP1 by covalently binding to cysteine 151. [17] However, the flexible linear long‐chain in the sulforaphane molecule and the presence of the isothiocyanate group make it target cysteine thiols in numerous proteins,[ 18 , 19 ] resulting in undesirable side effects. [20] In this study, we have attempted to develop non‐electrophilic NRF2 activators by 1) restricting the chain‘s flexibility in sulforaphane via cyclisation to a cyclohexane ring and 2) replacing the isothiocyanate group in sulforaphane with amino thiophene.…”

Characterization of interaction property of multi‐components in Gardenia jasminoides with aldose reductase by microdialysis combined with liquid chromatography coupled to mass spectrometry

“…Hence, microdialysis coupled to liquid chromatography/quadrupole time‐of‐flight tandem mass spectrometry (LC/QTOF‐MS/MS) was proposed to screen and identify active compounds of TCMs, which overcame the limitations of the screening assays in vitro . Microdialysis combined with HPLC has been developed to study the binding behaviors of drugs and metal ions to proteins in vitro . The probe membrane with typical molecular weight cut‐off excludes macromolecules such as proteins from samples.…”