Insights into the activation of oral keratinocyte cell death by <i>Candida albicans</i> and <i>Staphylococcus aureus</i> biofilms

Dias, Kássia de Carvalho; Barbugli, Paula Aboud; Vergani, Carlos Eduardo

doi:10.1080/08927014.2021.1994959

Cited by 2 publications

(1 citation statement)

References 26 publications

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“…In addition, Colombo et al [ 42 ] found that the proportion of Staphylococcus aureus and Enterococcus faecalis colonization on the buccal and gingival membrane was significantly higher in subjects with periodontitis than that in healthy individuals. Polymicrobial biofilms comprising Candida albicans and Staphylococcus aureus promote oral keratinocyte cell death, apoptosis, and/or necrosis, that increase the frequency and severity of oral diseases [ 43 ]. Therefore, it is reasonable to presume that yeasts and bacteria that exist in dental plaque aggravate erosive non-gingival OLP ( Figure 2 ).…”

Section: Discussionmentioning

confidence: 99%

Synergistic Efficacy of Plaque Control with Intralesional Triamcinolone Acetonide Injection on Erosive Non-Gingival Oral Lichen Planus: A Randomized Controlled Clinical Trial

Zhao

Lin

Deng

et al. 2022

IJERPH

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This study is the first time to assess the synergistic efficacy and safety of plaque control on erosive non-gingival oral lichen planus (OLP). A randomized, controlled, clinical trial with blind evaluation was designed, and 48 OLP patients with erosive non-gingival OLP lesions were randomly assigned to the experimental group (n = 25, receiving intralesional triamcinolone acetonide injection, periodontal scaling, and oral hygiene instruction) and the control group (n = 23, only receiving intralesional triamcinolone acetonide injection) once a week for 2 weeks. Erosion size, pain level, plaque index, and community periodontal index were measured at every visit. Patients cured of erosion were followed up for 3 months to evaluate the recurrence rate. Adverse reactions were also recorded. At day 14 ± 2, the experimental group showed a higher completely healed percentage of erosion, a greater reduction of erosion size and pain level. However, no significant difference was observed in the recurrence rate. No participants had any severe adverse reactions. In conclusion, an improvement was observed in patients with plaque control, and future studies with larger sample sizes are needed to reinforce the external validity of this study.

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Section: Discussionmentioning

confidence: 99%

Synergistic Efficacy of Plaque Control with Intralesional Triamcinolone Acetonide Injection on Erosive Non-Gingival Oral Lichen Planus: A Randomized Controlled Clinical Trial

Zhao

Lin

Deng

et al. 2022

IJERPH

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A customizable and defined medium supporting culturing of Candida albicans , Staphylococcus aureus , and human oral epithelial cells

Pasman,

Zhang,

Zaat

et al. 2024

Appl Environ Microbiol

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Candida albicans, an opportunistic oral pathogen, synergizes with Staphylococcus aureus , allowing bacteria to co-invade and systemically disseminate within the host. Studying human–microbe interactions creates the need for a universal culture medium that supports fungal, bacterial, and human cell culturing, while allowing sensitive analytical approaches such as OMICs and chromatography techniques. In this study, we established a fully defined, customizable adaptation of Dulbecco’s modified Eagle medium (DMEM), allowing multi-kingdom culturing of S. aureus , C. albicans , and human oral cell lines, whereas minimal version of DMEM (mDMEM) did not support growth of S. aureus , and neither did supplementation with dextrose, MEM non-essential amino acids, pyruvate, and Glutamax. This new medium composition, designated as “mDMEM-DMP,” promoted growth of all tested S. aureus strains. Addition of 25 mM 4-(2-hydroxyethyl)−1-piperazineethanesulfonic acid (HEPES) further improved growth, while higher concentrations did not improve growth any further. Higher concentrations of HEPES did result in prolonged stabilization of medium pH. mDMEM-DMP promoted (hyphal) C. albicans monoculturing and co-culturing on both solid and semi-solid surfaces. In contrast to S. aureus , addition of HEPES reduced C. albicans maximum culture optical density (OD). Finally, only buffered mDMEM-DMP (100 mM HEPES) was successful in maintaining the metabolic activity of human oral Ca9-22 and HO1N1 cell lines for 24 hours. Altogether, our findings show that mDMEM-DMP is a versatile and potent culture medium for both microbial and human cell culturing, providing a customizable platform to study human as well as microbial molecular physiology and putative interactions. IMPORTANCE Interaction between microbes and the host are in the center of interest both in disease and in health. In order to study the interactions between microbes of different kingdoms and the host, alternative media are required. Synthetic media are useful as they allow addition of specific components. In addition, well-defined media are required if high-resolution analyses such as metabolomics and proteomics are desired. We describe the development of a synthetic medium to study the interactions between C. albicans, S. aureus, and human oral epithelial cells. Our findings show that mDMEM-DMP is a versatile and potent culture medium for both microbial and human cell culturing, providing a customizable platform to study human as well as microbial molecular physiology and putative interactions.

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Insights into the activation of oral keratinocyte cell death by Candida albicans and Staphylococcus aureus biofilms

Cited by 2 publications

References 26 publications

Synergistic Efficacy of Plaque Control with Intralesional Triamcinolone Acetonide Injection on Erosive Non-Gingival Oral Lichen Planus: A Randomized Controlled Clinical Trial

Synergistic Efficacy of Plaque Control with Intralesional Triamcinolone Acetonide Injection on Erosive Non-Gingival Oral Lichen Planus: A Randomized Controlled Clinical Trial

A customizable and defined medium supporting culturing of Candida albicans , Staphylococcus aureus , and human oral epithelial cells

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