Insights into spermatogenesis in the migratory locust, Locusta migratoria (Linnaeus, 1758) (Orthoptera: Acrididae), following histological and ultrastructural features of the testis

Arafat, Esraa A.; El-Samad, Lamia M.; Moussian, Bernard; Hassan, Mohamed A.

doi:10.1016/j.micron.2023.103502

Cited by 4 publications

(1 citation statement)

References 32 publications

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“…To survey the microstructure of adult flies, the samples were prepared as previously reported 24 , 25 with slight adaptations. Briefly, the specimens were euthanized, washed with a normal saline solution several times, and then preserved in 70% ethanol for 24 h before being dried using a critical point dryer (Minnesota, USA).…”

Section: Methodsmentioning

confidence: 99%

Scuttle fly Megaselia scalaris (Loew) (Diptera: Phoridae) endoparasitoid as a novel biocontrol agent against adult American cockroaches (Periplaneta americana)

Arafat,

El-Samad,

Hassan

2024

Sci Rep

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The American cockroach, Periplaneta americana (Linnaeus, 1758) (Blattodea: Blattidae), is one of the most common pests that thrive in diverse environments and carries various pathogens, causing critical threats to public health and the ecosystem. We thus report in this study the first observation of decapitated American cockroaches as a result of infestation with scuttle fly parasitoids. Interestingly, behavioral alterations in the form of zombification-like behavior could be observed in cockroaches reared in the laboratory before being decapitated, implying that the insect targets cockroach heads. To identify this parasitoid, cockroaches’ corpora were isolated in jars, and apodous larvae were observed. Larvae developed into small coarctate pupae, and adults emerged. The scuttle flies were collected and exhibited tiny black, brown, to yellowish bodies. The fly was initially identified based on its morphological properties as a member of the order Diptera, family Phoridae. To provide further insights into the morphological attributes of the phorid species, the fly was examined using a scanning electron microscope (SEM) and then identified as Megaselia scalaris accordingly. SEM analysis revealed the distinctive structure of M. scalaris concerning the head, mouth parts, and legs. Specifically, the mouth parts include the labrum, labellum, rostrum, and maxillary palps. Although further investigations are still required to understand the complicated relationships between M. scalaris and American cockroaches, our findings provide a prominent step in the control of American cockroaches using M. scalaris as an efficient biological control agent.

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Section: Methodsmentioning

confidence: 99%