Insights into Role of Bromodomain, Testis-specific (Brdt) in Acetylated Histone H4-dependent Chromatin Remodeling in Mammalian Spermiogenesis

Dhar, Surbhi; Thota, Anusha; M, Rao

doi:10.1074/jbc.m111.288167

Cited by 63 publications

(67 citation statements)

References 75 publications

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“…The mechanism of BRDT action seems to entail recognition of acetylated chromatin via BD1 in elongating spermatids, followed by a process the investigators termed ''chromatin squeezing'' through interaction of two (or more) BRDT molecules, which would facilitate histone eviction. A similar observation was also reported in rat spermatids (Dhar et al 2012), but an indirect effect of Brdt on histone eviction cannot be excluded.…”

Section: Chromatin Post-translational Modifications (Ptms) In Post-mesupporting

confidence: 62%

How mammals pack their sperm: a variant matter

Bošković

Torres-Padilla

2013

Genes Dev.

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Producing competent gametes is essential for transmitting genetic information throughout generations. Spermatogenesis is a unique example of rearrangements of genome packaging to ensure fertilization. After meiosis, spermatids undergo drastic morphological changes, perhaps the most dramatic ones occurring in their nuclei, including the transition into a protamine-packaged genome. In this issue of Genes & Development, Montellier and colleagues (pp. 1680–1692) shed new light on the molecular mechanisms regulating this transition by ascribing for the first time a function to a histone variant, TH2B, in the regulation of this process.

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Section: Chromatin Post-translational Modifications (Ptms) In Post-mesupporting

confidence: 62%

How mammals pack their sperm: a variant matter

Bošković

Torres-Padilla

2013

Genes Dev.

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“…Adult Brdt À/À mouse testes lack post-meiotic germ cells [Dhar et al 2012;Gaucher et al 2012;Jacobson et al 2000;Plaseski et al 2012;Pivot-Pajot et al 2003]. In this study, we genotyped 9 RNF8 SNPs and 5 BRDT SNPs to investigate whether genetic changes of the SNPs were risk factors for NOA.…”

Section: Introductionmentioning

confidence: 99%

Genetic association study ofRNF8andBRDTvariants with non-obstructive azoospermia in the Chinese Han population

Zhang

Song

et al. 2014

Systems Biology in Reproductive Medicine

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Increasing evidence indicates that polymorphisms in genes relevant to spermatogenesis might modulate the efficiency of reproduction in men. Ring finger protein 8 (RNF8) and bromodomain testis-specific (BRDT) are two candidate genes associated with spermatogenesis. Here, we considered potential associations of 14 single nucleotide polymorphisms (SNPs) in RNF8 and BRDT genes in Chinese patients with non-obstructive azoospermia (NOA). We analyzed 361 men with NOA and 368 fertile controls by using Sequenom iplex technology. Our data did not reveal any variants associated with NOA susceptibility. However, we observed that rs104669 and rs195432 of RNF8 were in strong linkage disequilibrium. Haplotype analysis of the two SNPs indicated that the haplotype AC reduced the risk of NOA and the haplotype TC significantly evaluated the risk of NOA. Moreover, the RNF8 variants rs195432 (C/A p ¼ 0.030), rs195434 (T/C p ¼ 0.025), and rs2284922 (T/C p ¼ 0.034) were correlated with the smaller testis volume.Abbreviations: RNF8: ring finger protein 8; BRDT: bromodomain testis-specific; SNPs: single nucleotide polymorphisms; NOA: non-obstructive azoospermia; HWE: Hardy Weinberg Equilibrium; OR: odds ratio; LD: linkage disequilibrium; MAF: minor allele frequency(ies) Keywords BRDT, male infertility, non-obstructive azoospermia, RNF8, single nucleotide polymorphisms (SNPs) History

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“…At the molecular level, Brdt interacts with Smarce1, a component of the ATP-dependent SWI/SNF family of chromatin remodeling complexes that function to destabilize histone-DNA interaction. In spermatid nuclei, the interaction between Smarce1 and Brdt is enhanced by histone H4 acetylation [30]. Meanwhile, Brdt can interact with itself [30,31].…”

Section: Biological Significance Of Protein Acetylation In Spermatogementioning

confidence: 97%

“…In spermatid nuclei, the interaction between Smarce1 and Brdt is enhanced by histone H4 acetylation [30]. Meanwhile, Brdt can interact with itself [30,31]. Under conditions that promote histone acetylation, Brdt molecules interact in a head-to-tail manner, which identifies its directional alignment and probable "polymerization" on chromatin [31].…”

Section: Biological Significance Of Protein Acetylation In Spermatogementioning

confidence: 99%

“…Indeed, recent studies have pointed out that histone H4 hyperacetylation plays the pivotal role in initiation of histone displacement, and the functional link between histone H4 hyperacetylation and histone displacement resides in the testis-specific bromodomain protein Brdt. Brdt binds acetylated histone H4 through its bromodomains [28,29], and reorganizes acetylated chromatin in cell culture [28] and in round spermatid nuclei of the rat [30]. Specifically, the ablation of the first bromodomain of Brdt prevents histone displacement and genome compaction [31], and leads to sterility in male mice [32].…”

Section: Biological Significance Of Protein Acetylation In Spermatogementioning

confidence: 99%

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Protein Acetylation and Spermatogenesis

Pang

2013

Reproductive Sys Sexual Disord

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Spermatogenesis refers to the developmental process of male germ cell formation from the spermatogonial stem cell to mature spermatozoa. The progression of male germ cells through the different phases of development, along with changes in cellular size and morphology, involves a coordinated change in their gene expression program at both the transcript and protein levels. It is well known that the stability, biological activity and cellular localization of proteins are regulated by post-translational modifications. In this review, we provide a brief update of current knowledge about the role of protein acetylation in mammalian spermatogenesis. Based on recent findings specific examples were cited to illustrate how these modifications are involved in controlling the different events that are important to the proper development of male germ cells.

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Insights into Role of Bromodomain, Testis-specific (Brdt) in Acetylated Histone H4-dependent Chromatin Remodeling in Mammalian Spermiogenesis

Cited by 63 publications

References 75 publications

How mammals pack their sperm: a variant matter

How mammals pack their sperm: a variant matter

Genetic association study ofRNF8andBRDTvariants with non-obstructive azoospermia in the Chinese Han population

Protein Acetylation and Spermatogenesis

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