Insights into regulatory roles of MAPK-cascaded pathways in multiple stress responses and life cycles of insect and nematode mycopathogens

Tong, Sen‐Miao; Feng, Ming‐Guang

doi:10.1007/s00253-018-9516-1

Cited by 66 publications

(59 citation statements)

References 78 publications

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“…As a classic insect mycopathogen, B. bassiana has been widely applied for arthropod pest control. Its biological control potential depends mainly on an ability to infect the host through the normal route of cuticular penetration for entry into host hemocoel (22), where the penetrating hyphae turn into unicellular blastospores (also called hyphal bodies) to proliferate rapidly by yeast-like budding until host mummification to death (23)(24)(25)(26)(27), as well as an ability to overcome stress cues from host immunity defense and environment (28,29). Our fungal genome survey revealed a wide existence of orthologous Ssr4 in filamentous fungi.…”

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confidence: 99%

Nuclear Ssr4 Is Required for the In Vitro and In Vivo Asexual Cycles and Global Gene Activity of Beauveria bassiana

et al. 2020

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Ssr4 is known to serve as a cosubunit of chromatin-remodeling SWI/SNF and RSC complexes in yeasts but has not been functionally characterized in fungi. This study unveils for the first time the pleiotropic effects caused by deletion of ssr4 and its role in mediating global gene expression in a fungal insect pathogen. Our findings confirm an essential role of Ssr4 in hydrophobin biosynthesis and assembly required for growth, differentiation, and development of aerial hyphae for conidiation and conidial adhesion to insect surface and its essentiality for insect pathogenicity and virulence-related cellular events. Importantly, Ssr4 can regulate nearly one-fourth of all genes in the fungal genome in direct and indirect manners, including dozens involved in gene activity and hundreds involved in metabolism and/or transport of carbohydrates, amino acids, lipids, and/or inorganic ions. These findings highlight a significance of Ssr4 for filamentous fungal lifestyle.

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Nuclear Ssr4 Is Required for the In Vitro and In Vivo Asexual Cycles and Global Gene Activity of Beauveria bassiana

et al. 2020

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“…The biological control potential of an insect mycopathogen against insect pests is dependent on conidiation capacity and conidial quality represented by viability, infectivity, stress tolerance, and virulence because aerial conidia serve as active ingredients of most fungal insecticides (Tong & Feng, ; Zhang & Feng, ). For this reason, conidiation capacity of each strain was assessed during 11‐day incubation at 25°C in a light/dark cycle of 12:12 hr on SDAY plates, which were uniformly spread with 100‐μl aliquots of a 10 7 conidia ml −1 suspension for culture initiation.…”

Section: Resultsmentioning

confidence: 99%

“…Finally, stress‐responsive MAPK cascades are main mechanisms by which filamentous fungi adapt to hosts and environments (Tong & Feng, ). However, there are only a very few sensors that have been validated to function upstream of so important signalling cascades, such as one or two HKs as sensors of Hog1 cascade and two or three WSC proteins as sensors of Slt2 cascade.…”

Section: Discussionmentioning

confidence: 99%

“…The yeast Hog1 cascade is activated through the branch of the unique histidine kinase (HK) Sln1 (Hohmann, Krantz, & Nordlander, ; O'Rourke, Herskowitz, & O'Shea, ; Posas & Saito, ) or the branch of the upstream plasma membrane protein Sho1 in response to stress cues (Raitt, Posas, & Saito, ; Tatebayashi et al, ). In filamentous fungi, the Sln1‐like branch usually functions in the HOG pathway and mediates hallmarking osmotolerance positively and fludioxonil fungicide resistance negatively (Tong & Feng, ). However, homologous Sln1 (group VI HK) does not act as a sensor in filamentous fungi, which have dozens of hybrid HKs fallen into distinct groups (Bahn, ; Catlett, Yoder, & Turgeon, ; Defosse et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…The Sho1 branch was rarely shown to function in filamentous fungal osmoregulation. The non‐essentiality has been clarified in B. bassiana (Liu, Wang, Sun, Ying, & Feng, ), a filamentous fungal insect pathogen that usually undergoes asexual cycle, has the broadest host spectrum, and serves as one of main sources of fungal insecticides (Tong & Feng, ).…”

Section: Introductionmentioning

confidence: 99%

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The DUF1996 and WSC domain‐containing protein Wsc1I acts as a novel sensor of multiple stress cues in Beauveria bassiana

Tong

Wang

Gao

et al. 2019

Cellular Microbiology

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Wsc1I homologues featuring both an N‐terminal DUF1996 (domain of unknown function 1996) and a C‐terminal WSC (cell wall stress‐responsive component) domain exist in filamentous fungi but have never been functionally characterized. Here, Wsc1I is shown to localize in the vacuoles and cell wall/membrane of the insect mycopathogen Beauveria bassiana and hence linked to cell membrane‐ and vacuole‐related cellular events. In B. bassiana, deletion of Wsc1I resulted in marked increases of hyphal and conidial sensitivities to hyperosmotic agents, oxidants, cell wall perturbing chemicals, and metal cations (Cu2+, Zn2+, Fe2+, and Mg2+) despite slight impact on normal growth and conidiation. Conidia produced by the deletion mutant showed not only reduced tolerance to both 45°C heat and UVB irradiation but also attenuated virulence to a susceptible insect through normal cuticle infection or cuticle‐bypassing infection. Importantly, phosphorylation of the mitogen‐activated protein kinase Hog1 was largely attenuated or nearly abolished in the Wsc1I‐free cells triggered with hyperosmotic, oxidative, or cell wall perturbing stress. All changes were well restored by targeted gene complementation. Our findings highlight a novel role of Wsc1I in sensing multiple stress cues upstream of the Hog1 signalling pathway and its pleiotropic effects in B. bassiana.

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Colony heating protects honey bee populations from a risk of contact with wide‐spectrum Beauveria bassiana insecticides applied in the field

Peng

Tong

Zeng

et al. 2020

Pest Management Science

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BACKGROUNDThe safety of fungal insecticides to apiculture is a public concern but remains poorly understood. This study seeks to evaluate whether, how and why wide‐spectrum Beauveria bassiana insecticides are safe to honey bees in a novel assessment system.RESULTSMesonotum dipping with a 108 conidia ml−1 suspension and body contact with conidial suspension in sucrose solution caused high mortalities of adult forager bees at 25 °C optimal for conidial germination and hyphal invasion. Intriguingly, colony sizes in the hives contaminated by the forager bees contacting viable and inactivated conidia at two sites (1.2 km in distance), respectively, showed similar increase percentages (31.7% versus 29.2%) during a 4‐week summer period of exposure to environment. No sign of fungal infection was found within each of the monitored colonies. Neither was fungal outgrowth observed on surfaces of bee cadavers cleaned from each hive at either site. Hourly counts of cleaned cadavers from videotapes presented no significant difference in colony‐cleaning behavior between the two sites. During the period, in‐hive temperatures at both sites were persistently stabilized at approximately 35 °C, which abolished conidial germination and were far above the out‐hive temperature range.CONCLUSIONIt is colony heating that protects honey bee populations from a risk of forager bees' contact with formulated conidia applied for arthropod pest control. No role was detected for colony self‐cleaning behavior in protecting the bee colonies from the risk. © 2020 Society of Chemical Industry

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Insights into regulatory roles of MAPK-cascaded pathways in multiple stress responses and life cycles of insect and nematode mycopathogens

Cited by 66 publications

References 78 publications

Nuclear Ssr4 Is Required for the In Vitro and In Vivo Asexual Cycles and Global Gene Activity of Beauveria bassiana

Nuclear Ssr4 Is Required for the In Vitro and In Vivo Asexual Cycles and Global Gene Activity of Beauveria bassiana

The DUF1996 and WSC domain‐containing protein Wsc1I acts as a novel sensor of multiple stress cues in Beauveria bassiana

Colony heating protects honey bee populations from a risk of contact with wide‐spectrum Beauveria bassiana insecticides applied in the field

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