Insights into polyester plastic biodegradation by carboxyl ester hydrolases

Gricajeva, Alisa; Nadda, Ashok Kumar; Gudiukaitė, Renata

doi:10.1002/jctb.6745

Cited by 54 publications

(36 citation statements)

References 270 publications

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“…The protein RPA1511 isolated from Rhodopseudomonas palustris has the catalytic triad residues, which are important for the hydrolysis of both monoester and polyester substrates. It was considered to be critical for the hydrolysis of PLA and was active in catalyzing solid PLA with the production of lactic acid monomers, dimers, and larger oligomers ( Hajighasemi et al, 2016 ; Gricajeva et al, 2021 ). Moreover, some functions related to the carbon, nitrogen, and sulfur metabolic pathways were revealed by FAPROTAX, which indicated that BDMs may affect the biogeochemical cycling of elements in agroecosystems.…”

Section: Discussionmentioning

confidence: 99%

The Succession of Bacterial Community Attached on Biodegradable Plastic Mulches During the Degradation in Soil

Feng

et al. 2021

Front. Microbiol.

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Despite the increasing application of biodegradable plastic mulches (BDMs) in agriculture, the colonization and succession of the attached microbial community on BDMs during their degradation processes remain poorly characterized. Here, we buried four types of commonly used BDMs, including pure polylactic acid (PLA), pure polybutylene adipate terephthalate (PBAT), and two mixtures of PLA and PBAT (85:15 and 15:85 w/w), and one classic polyethylene (PE) mulch in soil for 5 months. Both plastic components and incubation time significantly shaped the β-diversities of microbiota on the plastic mulches (p < 0.001). Meanwhile, the microbial compositions and community structures on BDMs were significantly different from PE mulch, and when excluding PE mulch, the microbiota varied more with time than by the composition of the four BDMs. The orders Burkholderiales and Pseudonocardiales were dominant on most BDMs across different time points. The genus Ramlibacter was revealed as a common biomarker for both PLA and PBAT by random-forest model, and all biomarkers for the BDMs belonged to the dominant order Burkholderiales. In addition, degradation-related and pathogen-related functional taxa were enriched in all mulches among all 40 functional groups, while surprisingly, potential pathogens were detected at higher levels on BDMs than PE. For community assembly on all mulches, the drift and dispersal processes played more important roles than selection, and in particular, the contribution of stochastic drift increased during the degradation process of BDMs while selection decreased, while the opposite trend was observed with PE mulch. Overall, our results demonstrated some degradation species and pathogens were specifically enriched on BDMs, though stochastic processes also had important impacts on the community assembly. It suggested that, similar to conventional plastic mulch, the increased usage of BDMs could lead to potential hazards to crops and human health.

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Section: Discussionmentioning

confidence: 99%

The Succession of Bacterial Community Attached on Biodegradable Plastic Mulches During the Degradation in Soil

Feng

et al. 2021

Front. Microbiol.

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“…Synthetic polyester plastics may be hydrolyzed by microbial lipases, carboxylesterases, and cutinases, which can therefore be collectively referred to as polyesterases ( Gricajeva et al, 2021 ). Many polyesterases are PET hydrolyzing enzymes (PHEs), although cutinases are considered most effective in cleaving polyester bond linkages ( Kawai et al, 2020 ).…”

Section: Resultsmentioning

confidence: 99%

Identification of BgP, a Cutinase-Like Polyesterase From a Deep-Sea Sponge-Derived Actinobacterium

et al. 2022

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Many marine bacteria produce extracellular enzymes that degrade complex molecules to facilitate their growth in environmental conditions that are often harsh and low in nutrients. Marine bacteria, including those inhabiting sea sponges, have previously been reported to be a promising source of polyesterase enzymes, which have received recent attention due to their potential ability to degrade polyethylene terephthalate (PET) plastic. During the screening of 51 marine bacterial isolates for hydrolytic activities targeting ester and polyester substrates, a Brachybacterium ginsengisoli B129SM11 isolate from the deep-sea sponge Pheronema sp. was identified as a polyesterase producer. Sequence analysis of genomic DNA from strain B129SM11, coupled with a genome “mining” strategy, allowed the identification of potential polyesterases, using a custom database of enzymes that had previously been reported to hydrolyze PET or other synthetic polyesters. This resulted in the identification of a putative PET hydrolase gene, encoding a polyesterase-type enzyme which we named BgP that shared high overall similarity with three well-characterized PET hydrolases—LCC, TfCut2, and Cut190, all of which are key enzymes currently under investigation for the biological recycling of PET. In silico protein analyses and homology protein modeling offered structural and functional insights into BgP, and a detailed comparison with Cut190 revealed highly conserved features with implications for both catalysis and substrate binding. Polyesterase activity was confirmed using an agar-based polycaprolactone (PCL) clearing assay, following heterologous expression of BgP in Escherichia coli. This is the first report of a polyesterase being identified from a deep-sea sponge bacterium such as Brachybacterium ginsengisoli and provides further insights into marine-derived polyesterases, an important family of enzymes for PET plastic hydrolysis. Microorganisms living in association with sponges are likely to have increased exposure to plastics and microplastics given the wide-scale contamination of marine ecosystems with these plastics, and thus they may represent a worthwhile source of enzymes for use in new plastic waste management systems. This study adds to the growing knowledge of microbial polyesterases and endorses further exploration of marine host-associated microorganisms as a potentially valuable source of this family of enzymes for PET plastic hydrolysis.

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“…Additionally, in this special issue, the degradation and conversion of various emerging pollutants, including polyaromatic hydrocarbons, poly (ε-caprolactone), 5-hydroxymethyl furfural, plastic, polyesters, using microbes and enzymes have been well described. [1][2][3][4][5][6][7][8][9][10][11][12] In addition to this various biomass, biopolymers including lignin, cellulose, and municipal-, industrial-, and agricultural-based pollutants were documented to convert into valuable products using enzymatic pathways. [13][14][15][16] The cutting-edge technologies of protein engineering and directed evolution have already minimized the cost of enzymatic transformation processes with higher product yield.…”

Section: Microbial Enzymes For Green Energy and Clean Environmentmentioning

confidence: 99%