Insights into nitrogen and phosphorus metabolic mechanisms of algal-bacterial aerobic granular sludge via metagenomics: Performance, microbial community and functional genes

Xiong, Wei; Wang, Shaojie; Jin, Yu; Wu, Zhiqing; Liú, Dan; Su, Haijia

doi:10.1016/j.biortech.2022.128442

Cited by 45 publications

(10 citation statements)

References 46 publications

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“…The specific functions of microbial communities (such as nitrogen and phosphorus conversion) were closely related to metabolic pathways (Xiong et al, 2023 ). The results showed that the KO pathway ( Supplementary Figures S4 , S5 ) involved in the nitrogen and phosphorus cycle was highly expressed in lake water.…”

Section: Resultsmentioning

confidence: 99%

Unraveling the rate-limiting step in microorganisms' mediation of denitrification and phosphorus absorption/transport processes in a highly regulated river-lake system

Ding,

Yang,

Liu

et al. 2023

Front. Microbiol.

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River–lake ecosystems are indispensable hubs for water transfers and flow regulation engineering, which have frequent and complex artificial hydrological regulation processes, and the water quality is often unstable. Microorganisms usually affect these systems by driving the nutrient cycling process. Thus, understanding the key biochemical rate-limiting steps under highly regulated conditions was critical for the water quality stability of river–lake ecosystems. This study investigated how the key microorganisms and genes involving nitrogen and phosphorus cycling contributed to the stability of water by combining 16S rRNA and metagenomic sequencing using the Dongping river–lake system as the case study. The results showed that nitrogen and phosphorus concentrations were significantly lower in lake zones than in river inflow and outflow zones (p < 0.05). Pseudomonas, Acinetobacter, and Microbacterium were the key microorganisms associated with nitrate and phosphate removal. These microorganisms contributed to key genes that promote denitrification (nirB/narG/narH/nasA) and phosphorus absorption and transport (pstA/pstB/pstC/pstS). Partial least squares path modeling (PLS-PM) revealed that environmental factors (especially flow velocity and COD concentration) have a significant negative effect on the key microbial abundance (p < 0.001). Our study provides theoretical support for the effective management and protection of water transfer and the regulation function of the river–lake system.

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Section: Resultsmentioning

confidence: 99%

Unraveling the rate-limiting step in microorganisms' mediation of denitrification and phosphorus absorption/transport processes in a highly regulated river-lake system

Ding,

Yang,

Liu

et al. 2023

Front. Microbiol.

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“…In Stage IV, the EPS in algal-bacterial AGS and bacterial AGS progressively reduced to its initial values. In the whole process, PN was the main composition of EPS, which ensures the formation and stability of algal-bacterial AGS [30]. A previous study also proved that a reduction in PS would reduce sludge bulking, and the decline in settleability and the entanglement of filamentous bacteria around the surfaces of the particles may be related to the large amount of PS secreted by algal-bacterial AGS [35].…”

Section: Analysis Of Extracellular Polymeric Substancesmentioning

confidence: 88%

“…Two reactors continued to show this gap in Stage IV until the end of the experiment, indicating that algal-bacterial AGS could maintain adequate phosphorus removal efficiency even in the face of a high surfactant concentration. Many studies have revealed that algal-bacterial AGS is indeed more effective than bacterial AGS in removing phosphorus [11,[30][31][32]. Firstly, the particles formed by the coupling of algae and AGS make PAOs better aggregated.…”

Section: The Removal Of Pmentioning

confidence: 99%

Performance of Treating Surfactant-Containing Wastewater by Using Algal–Bacterial Granular Sludge

Wang,

Liu,

Hang

2024

Water

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Surfactants have been widely used in various fields in recent years, but subsequent treatment of their wastewater has always been a problem that needs to be solved. As a new sewage treatment method, algal–bacterial aerobic granule sludge (algal–bacterial AGS) is considered to be one of the potential methods for treating this kind of wastewater. This study investigated the efficiency of using algal–bacterial AGS to treat wastewater containing surfactants and compared it with bacterial AGS. The results indicated that when confronting a high-concentration surfactant, algal–bacterial AGS could keep a relatively high nutrient removal capacity (about 90% dissolved organic carbon, 99% ammonia nitrogen, 52% total phosphorus) and improved the adaptability to sudden changes in high-concentration surfactant environments compared with bacterial AGS. These results illustrated that algal–bacterial AGS is a potential method to process wastewater containing surfactants with superior treatment efficiency and adaption response.

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“…The dominant bacteria belonging to the phyla Proteobacteria, Bacteroidota and Nitrospirae are generally associated with the sludge aggregation, EPS secretion and nutrient removal. 31 It is worth noting that Cyanobacteria, as a special prokaryote, were observed in the algal−bacterial symbiotic systems (R2 and R3). Under nutrient limitation conditions, the unique growth advantage of Cyanobacteria confers them distinct merits over eukaryotic algae in their enrichment under illumination conditions.…”

Section: Microbial Community Shiftmentioning

confidence: 99%

Zero-Valent Iron Enhances Nutrient Removal and Long-Term Stability of Algal–Bacterial Granular Sludge under Low Carbon Conditions

Fan,

Zhang,

Lens

et al. 2024

ACS EST Water

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Achieving stable wastewater treatment performance of algal−bacterial granular sludge (ABGS) remains challenging under low-carbon conditions. Iron plays a pivotal role in enhancing microbial metabolism under low carbon conditions. Herein, zerovalent iron (ZVI) was applied to expedite the granulation process and enhance the sludge stability and nutrient removal of the ABGS system. ZVI served as a carrier facilitating the adhesion of algae and bacteria, subsequently stimulating the secretion of extracellular polymeric substance (EPS) to expedite microbial aggregation, ultimately augmenting the granular size, sludge density, and settleability of ABGS. Moreover, ZVI suppressed the growth of filamentous bacteria (e.g., Neomegalonema) and phototrophs (e.g., Cyanobacteria), which was essential for maintaining ABGS stability during long-term operation. Compared to the aerobic granular sludge (AGS) and ABGS systems, the nitrogen and phosphorus removal efficiencies of ZVI-enhanced ABGS system were increased by 6.1−20.7 and 18.3−37.4%, respectively, probably due to the selective enrichment of bacteria and microalgae, the up-regulated functional enzymes (e.g., nitrite reductase, polyphosphate kinase), and the enhanced electron transport capacity and energy metabolism. This study aids in understanding the enhancement mechanism behind the improved treatment performance and stability of the ABGS system by ZVI, and provides a novel strategy for rapid development of ABGS under low carbon conditions.

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Insights into nitrogen and phosphorus metabolic mechanisms of algal-bacterial aerobic granular sludge via metagenomics: Performance, microbial community and functional genes

Cited by 45 publications

References 46 publications

Unraveling the rate-limiting step in microorganisms' mediation of denitrification and phosphorus absorption/transport processes in a highly regulated river-lake system

Unraveling the rate-limiting step in microorganisms' mediation of denitrification and phosphorus absorption/transport processes in a highly regulated river-lake system

Performance of Treating Surfactant-Containing Wastewater by Using Algal–Bacterial Granular Sludge

Zero-Valent Iron Enhances Nutrient Removal and Long-Term Stability of Algal–Bacterial Granular Sludge under Low Carbon Conditions

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