Insights into molecular and cellular mechanisms of hormonal actions on fish ion regulation derived from the zebrafish model

Guh, Ying-Jey; Hwang, Pung‐Pung

doi:10.1016/j.ygcen.2016.08.009

Cited by 36 publications

(30 citation statements)

References 96 publications

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“…The presence of apical ASIC4 (acid-sensing ion channel 4) has been shown in gill ionocytes of two teleost species, the rainbow trout (in PNA+ ionocytes) (Dymowska et al, 2014) and zebrafish (in HR cells) (Dymowska et al, 2015). Zebrafish HR cells express NHE3b in apical membranes suggesting colocalization of VHA, NHE3b and ASIC4 in this cell type (Guh and Hwang, 2017). ASIC4 could thus, beside NHE3b, be part of a secondary Na + uptake mechanism in these cells.…”

Section: Accepted Manuscriptmentioning

confidence: 95%

Ion uptake pathways in European sea bass Dicentrarchus labrax

Blondeau‐Bidet

Hiroi

Lorin‐Nebel

2019

Gene

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Section: Accepted Manuscriptmentioning

confidence: 95%

Ion uptake pathways in European sea bass Dicentrarchus labrax

Blondeau‐Bidet

Hiroi

Lorin‐Nebel

2019

Gene

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“…Five types of ionocytes have been identified in the skin of zebrafish embryos, including H + -ATPase-rich (HR) cells, Na + , K + -ATPase-rich (NaR) cells, Na + -Cl - cotransporter-expressing (NCC) cells, SLC26-expressing cells, and K + -secreting (KS) cells [3]. These ionocytes perform transepithelial H + secretion/Na + uptake/NH4 + excretion, Ca 2+ uptake, Na + /Cl - uptake, Cl - uptake/HCO 3 - secretion, and K + secretion, respectively, by utilizing various transporters located at the apical or basolateral cell surface [3–6].…”

Section: Introductionmentioning

confidence: 99%

Zebrafish Klf4 maintains the ionocyte progenitor population by regulating epidermal stem cell proliferation and lateral inhibition

Chen

Liao

et al. 2019

PLoS Genet

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In the skin and gill epidermis of fish, ionocytes develop alongside keratinocytes and maintain body fluid ionic homeostasis that is essential for adaptation to environmental fluctuations. It is known that ionocyte progenitors in zebrafish embryos are specified from p63 + epidermal stem cells through a patterning process involving DeltaC (Dlc)-Notch-mediated lateral inhibition, which selects scattered dlc + cells into the ionocyte progenitor fate. However, mechanisms by which the ionocyte progenitor population is modulated remain unclear. Krüppel-like factor 4 (Klf4) transcription factor was previously implicated in the terminal differentiation of mammalian skin epidermis and is known for its bifunctional regulation of cell proliferation in a tissue context-dependent manner. Here, we report novel roles for zebrafish Klf4 in the ventral ectoderm during embryonic skin development. We found that Klf4 was expressed in p63 + epidermal stem cells of the ventral ectoderm from 90% epiboly onward. Knockdown or knockout of klf4 expression reduced the proliferation rate of p63 + stem cells, resulting in decreased numbers of p63 + stem cells, dlc - p63 + keratinocyte progenitors and dlc + p63 + ionocyte progenitor cells. These reductions subsequently led to diminished keratinocyte and ionocyte densities and resulted from upregulation of the well-known cell cycle regulators, p53 and cdkn1a / p21 . Moreover, mutation analyses of the KLF motif in the dlc promoter, combined with VP16-klf4 or engrailed-klf4 mRNA overexpression analyses, showed that Klf4 can bind the dlc promoter and modulate lateral inhibition by directly repressing dlc expression. This idea was further supported by observing the lateral inhibition outcomes in klf4 -overexpressing or knockdown embryos. Overall, our experiments delineate novel roles for zebrafish Klf4 in regulating the ionocyte progenitor population throughout early stem cell stage to initiation of terminal differentiation, which is dependent on Dlc-Notch-mediated lateral inhibition.

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“…The processes in the uptake and acid-base regulation in zebrafish have been comprehensively discussed in several previous reviews [ 1 , 5 , 6 , 7 , 8 , 9 , 10 , 11 ]. However, no article has systematically reviewed the similarities and differences in the regulation of epithelial ion transporters between zebrafish and mammals during metabolic acidosis.…”

Section: Introductionmentioning

confidence: 99%

Zebrafish as a Model System for Investigating the Compensatory Regulation of Ionic Balance during Metabolic Acidosis

Lewis

Kwong

2018

IJMS

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Zebrafish (Danio rerio) have become an important model for integrative physiological research. Zebrafish inhabit a hypo-osmotic environment; to maintain ionic and acid-base homeostasis, they must actively take up ions and secrete acid to the water. The gills in the adult and the skin at larval stage are the primary sites of ionic regulation in zebrafish. The uptake of ions in zebrafish is mediated by specific ion transporting cells termed ionocytes. Similarly, in mammals, ion reabsorption and acid excretion occur in specific cell types in the terminal region of the renal tubules (distal convoluted tubule and collecting duct). Previous studies have suggested that functional regulation of several ion transporters/channels in the zebrafish ionocytes resembles that in the mammalian renal cells. Additionally, several mechanisms involved in regulating the epithelial ion transport during metabolic acidosis are found to be similar between zebrafish and mammals. In this article, we systemically review the similarities and differences in ionic regulation between zebrafish and mammals during metabolic acidosis. We summarize the available information on the regulation of epithelial ion transporters during acidosis, with a focus on epithelial Na+, Cl− and Ca2+ transporters in zebrafish ionocytes and mammalian renal cells. We also discuss the neuroendocrine responses to acid exposure, and their potential role in ionic compensation. Finally, we identify several knowledge gaps that would benefit from further study.

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Insights into molecular and cellular mechanisms of hormonal actions on fish ion regulation derived from the zebrafish model

Cited by 36 publications

References 96 publications

Ion uptake pathways in European sea bass Dicentrarchus labrax

Ion uptake pathways in European sea bass Dicentrarchus labrax

Zebrafish Klf4 maintains the ionocyte progenitor population by regulating epidermal stem cell proliferation and lateral inhibition

Zebrafish as a Model System for Investigating the Compensatory Regulation of Ionic Balance during Metabolic Acidosis

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