Insights into methanogenesis of mesophilic-psychrophilic varied anaerobic digestion of municipal sludge with antibiotic stress

Characteristics of Biogas Production Activity and Microbial Community during Sub-Moderate Temperature Anaerobic Digestion of Wastewater

“…The feasibility of long-term continuous LT-AD treatment of long-chain fatty acid (LCFA)-containing wastewater using acclimated biomass has been reported, with Methanocorpusculum playing a crucial role in LCFA degradation during both psychrophilic and mesophilic treatments [23]. It was observed by Hui Y et al [22] that the initial inhibition of methane production was observed in LT-AD under antibiotic stress (e.g., oxytetracycline); however, it was later stimulated at 35 • C; and in comparison to MT-AD, the dominant acetoclastic methanogens Methanosaeta gradually declined and were replaced by methylotrophic Methanomethylovorans in LT-AD. Additionally, Estefanny QC et al [24] reported that the microbial diversity decreased with successful adaptation to increasing total ammonia nitrogen in LT-AD, while the microbial community exhibited increased diversity under mesophilic conditions despite ammonia inhibition.…”

“…The acclimation of microbiomes to low temperature [18,19] and properties of psychrotrophs have been frequently studied [20,21]. Many studies have also compared the performance of low-and moderate-temperature AD [22]. The feasibility of long-term continuous LT-AD treatment of long-chain fatty acid (LCFA)-containing wastewater using acclimated biomass has been reported, with Methanocorpusculum playing a crucial role in LCFA degradation during both psychrophilic and mesophilic treatments [23].…”