Insights into mesophilic virulence, antibiotic resistant and human pathogenicity: A genomics study on the Aeromonas salmonicida SRW-OG1 newly isolated from the Asian fish Epinephelus coioides

Zhong, Yuanfu; Qi, Weilu; Xü, Wei; Zhao, Lingmin; Xiao, Binlong; Yan, Qingpi; Huang, Lixing

doi:10.1016/j.aquaculture.2021.736630

Cited by 15 publications

(11 citation statements)

References 43 publications

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“…The pathogenic strain isolated from the spleen of large yellow croaker with visceral white spot disease was later confirmed as P. plecoglossicida (NZBD9) ( Huang et al., 2018 ; Zhong et al., 2021 ). The strain was cultured in LB medium at 220 rpm at 28°C ( He et al., 2020 ).…”

Section: Methodsmentioning

confidence: 97%

Effect of Ferredoxin Receptor FusA on the Virulence Mechanism of Pseudomonas plecoglossicida

Wang

Lin

et al. 2022

Front. Cell. Infect. Microbiol.

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Pseudomonas plecoglossicida is an aerobic Gram-negative bacterium, which is the pathogen of “Visceral white spot disease” in large yellow croaker. P. plecoglossicida is a temperature-dependent bacterial pathogen in fish, which not only reduces the yield of large yellow croaker but also causes continuous transmission of the disease, seriously endangering the healthy development of fisheries. In this study, a mutant strain of fusA was constructed using homologous recombination technology. The results showed that knockout of P. plecoglossicida fusA significantly affected the ability of growth, adhesion, and biofilm formation. Temperature, pH, H2O2, heavy metals, and the iron-chelating agent were used to treat the wild type of P. plecoglossicida; the results showed that the expression of fusA was significantly reduced at 4°C, 12°C, and 37°C. The expression of fusA was significantly increased at pH 4 and 5. Cu2+ has a significant inducing effect on the expression of fusA, but Pb2+ has no obvious effect; the expression of fusA was significantly upregulated under different concentrations of H2O2. The expression of the fusA gene was significantly upregulated in the 0.5~4-μmol/l iron-chelating agent. The expression level of the fusA gene was significantly upregulated after the logarithmic phase. It was suggested that fusA included in the TBDR family not only was involved in the transport of ferredoxin but also played important roles in the pathogenicity and environment adaptation of P. plecoglossicida.

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Section: Methodsmentioning

confidence: 97%

Effect of Ferredoxin Receptor FusA on the Virulence Mechanism of Pseudomonas plecoglossicida

Wang

Lin

et al. 2022

Front. Cell. Infect. Microbiol.

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“…Since that time, the genomic sequences of several mesophilic strains of A. salmonicida have been published and investigated. While the early mesophilic strains had no known host, recently described strains have been isolated from a leech [17], human patients [18], and diseased fish [19] and birds [20].…”

Section: Aeromonas Salmonicida Subspecies: a Complex Taxonomic Dilemmamentioning

confidence: 99%

“…It also implies that we must remain open to the idea of finding mesophilic A. salmonicida strains in unexpected environments distinct from those where psychrophilic strains are found. Mesophilic strains may also be hidden in environments where psychrophilic strains are usually found; an example is strain SRW-OG1 isolated from fish [19]. To support the idea that mesophilic strains can inhabit cold environments, it is important to remember that these strains have been shown to have a higher growth capacity than typical psychrophilic strains at 18 • C and even 7 • C [15].…”

Section: A Potential Hidden Diversitymentioning

confidence: 99%

To Be or Not to Be Mesophilic, That Is the Question for Aeromonas salmonicida

Vincent

Charette

2022

Microorganisms

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The bacterium Aeromonas salmonicida has long been known to be one of the most feared pathogens in fish farming. However, the more we discover about this bacterial species, the more we question whether it is really exclusively an aquatic pathogen. In recent years, it has become obvious that this bacterial species includes a myriad of strains with various lifestyle and ecological niches, including the well-known strict psychrophiles, the first bacteria known of the species, and the newly described mesophilic strains. The mesophiles are able to grow at low temperatures, but even better at temperatures of approximately 37 °C, which strict psychrophiles cannot do. In this perspective article, we address some aspects surrounding this dual lifestyle in A. salmonicida, including the impact of mobile genetic elements, and how future research around this bacterial species may focus on the psychrophilic/mesophilic dichotomy, which makes A. salmonicida an increasingly interesting and relevant model for the study of speciation.

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“…The symptoms of infection are mainly “furunculosis” (skin ulcers) and ‘septicemia’ in salmons ( Salomón et al., 2021 ) and C. carpio ( Bhat et al., 2021 ). A. salmonicida SRW-OG1 was isolated in our laboratory from Epinephelus coioides suffering from furunculosis in Dongshan County, Zhangzhou City, Fujian Province ( Zhong et al., 2021 ). Surprisingly, the pathogen was isolated at 28°C.…”

Section: Introductionmentioning

confidence: 99%

The temperature-dependent expression of type II secretion system controls extracellular product secretion and virulence in mesophilic Aeromonas salmonida SRW-OG1

Chen

Cai

et al. 2022

Front. Cell. Infect. Microbiol.

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Aeromonas salmonicida is a typical cold water bacterial pathogen that causes furunculosis in many freshwater and marine fish species worldwide. In our previous study, the pathogenic A. salmonicida (SRW-OG1) was isolated from a warm water fish, Epinephelus coioides was genomics and transcriptomics analyzed. Type II secretion system was found in the genome of A. salmonicida SRW-OG1, while the expressions of tatA, tatB, and tatC were significantly affected by temperature stress. Also, sequence alignment analysis, homology analysis and protein secondary structure function analysis showed that tatA, tatB, and tatC were highly conservative, indicating their biological significance. In this study, by constructing the mutants of tatA, tatB, and tatC, we investigated the mechanisms underlying temperature-dependent virulence regulation in mesophilic A. salmonida SRW-OG1. According to our results, tatA, tatB, and tatC mutants presented a distinct reduction in adhesion, hemolysis, biofilm formation and motility. Compared to wild-type strain, inhibition of the expression of tatA, tatB, and tatC resulted in a decrease in biofilm formation by about 23.66%, 19.63% and 40.13%, and a decrease in adhesion ability by approximately 77.69%, 80.41% and 62.14% compared with that of the wild-type strain. Furthermore, tatA, tatB, and tatC mutants also showed evidently reduced extracellular enzymatic activities, including amylase, protease, lipase, hemolysis and lecithinase. The genes affecting amylase, protease, lipase, hemolysis, and lecithinase of A. salmonicida SRW-OG1 were identified as cyoE, ahhh1, lipA, lipB, pulA, HED66_RS01350, HED66_RS19960, aspA, fabD, and gpsA, which were notably affected by temperature stress and mutant of tatA, tatB, and tatC. All above, tatA, tatB and tatC regulate the virulence of A. salmonicida SRW-OG1 by affecting biofilm formation, adhesion, and enzymatic activity of extracellular products, and are simultaneously engaged in temperature-dependent pathogenicity.

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Insights into mesophilic virulence, antibiotic resistant and human pathogenicity: A genomics study on the Aeromonas salmonicida SRW-OG1 newly isolated from the Asian fish Epinephelus coioides

Cited by 15 publications

References 43 publications

Effect of Ferredoxin Receptor FusA on the Virulence Mechanism of Pseudomonas plecoglossicida

Effect of Ferredoxin Receptor FusA on the Virulence Mechanism of Pseudomonas plecoglossicida

To Be or Not to Be Mesophilic, That Is the Question for Aeromonas salmonicida

The temperature-dependent expression of type II secretion system controls extracellular product secretion and virulence in mesophilic Aeromonas salmonida SRW-OG1

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