Insights into genes involved in electricity generation in Geobacter sulfurreducens via whole genome microarray analysis of the OmcF-deficient mutant

Kim, Byoung-Chan; Postier, Bradley L.; DiDonato, Raymond J.; Chaudhuri, Suhnrita; Nevin, Kelly P.; Lovley, Derek R.

doi:10.1016/j.bioelechem.2008.04.023

Cited by 93 publications

(52 citation statements)

References 45 publications

(73 reference statements)

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“…Past studies had suggested that GSU1330 played a role in electron transfer to electrodes, as it is part of a gene cluster strongly upregulated in cells grown on the anode of a microbial fuel cell (12). In addition, GSU1330 was downregulated in an outer membrane cytochrome mutant with a decreased capacity for current production (16). However, no effect on electron transfer was observed when the GSU1330 mutant was examined under our controlled electrode growth conditions (Fig.…”

Section: Discussionmentioning

confidence: 69%

“…Many genes implicated in Fe(III) and electrode reduction were identified based on proteomic and microarray analysis of cultures grown with fumarate versus Fe(III) citrate as a terminal electron acceptor (9,15,35). More recently, similar expression data from Fe(III) oxide and electrode-grown cultures have also become available (8,12,16). In most extracellular electron transfer studies, outer membrane proteins (such as c-type cytochromes) have been the focus (4,23,27,32), leading to targeted knockout studies of at least 14 cytochromes to date.…”

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confidence: 99%

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Identification of Genes Involved in Biofilm Formation and Respiration via Mini- Himar Transposon Mutagenesis of Geobacter sulfurreducens

2009

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Electron transfer from cells to metals and electrodes by the Fe(III)-reducing anaerobe Geobacter sulfurreducens requires proper expression of redox proteins and attachment mechanisms to interface bacteria with surfaces and neighboring cells. We hypothesized that transposon mutagenesis would complement targeted knockout studies in Geobacter spp. and identify novel genes involved in this process. Escherichia coli mating strains and plasmids were used to develop a conjugation protocol and deliver mini-Himar transposons, creating a library of over 8,000 mutants that was anaerobically arrayed and screened for a range of phenotypes, including auxotrophy for amino acids, inability to reduce Fe(III) citrate, and attachment to surfaces. Following protocol validation, mutants with strong phenotypes were further characterized in a three-electrode system to simultaneously quantify attachment, biofilm development, and respiratory parameters, revealing mutants defective in Fe(III) reduction but unaffected in electron transfer to electrodes (such as an insertion in GSU1330, a putative metal export protein) or defective in electrode reduction but demonstrating wild-type biofilm formation (due to an insertion upstream of the NHL domain protein GSU2505). An insertion in a putative ATP-dependent transporter (GSU1501) eliminated electrode colonization but not Fe(III) citrate reduction. A more complex phenotype was demonstrated by a mutant containing an insertion in a transglutaminase domain protein (GSU3361), which suddenly ceased to respire when biofilms reached approximately 50% of the wild-type levels. As most insertions were not in cytochromes but rather in transporters, twocomponent signaling proteins, and proteins of unknown function, this collection illustrates how biofilm formation and electron transfer are separate but complementary phenotypes, controlled by multiple loci not commonly studied in Geobacter spp.

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Section: Discussionmentioning

confidence: 69%

mentioning

confidence: 99%

Identification of Genes Involved in Biofilm Formation and Respiration via Mini- Himar Transposon Mutagenesis of Geobacter sulfurreducens

2009

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“…Genetic studies of these bacteria revealed that these bacteria are able to transfer electrons to electrodes via c-type cytochromes displayed on the outer membrane of the bacteria [Holmes et al 2006;Bretschger et al 2007;Kim et al 2008]. Electron transfer is also established by electrical conductive pili, so called microbial nanowires [Reguera et al 2005;Gorby 2006;Reguera et al 2006;El-Naggar et al 2010].…”

Section: Microbial Fuel Cells : Microbial Production Of Electricitymentioning

confidence: 99%

Synthetic Biology and Microdevices

Venken

Marchal

Vanderleyden

2013

J. Emerg. Technol. Comput. Syst.

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Recent developments demonstrate that the combination of microbiology with micro-and nanoelectronics is a successful approach to develop new miniaturized sensing devices and other technologies. In the last decade, there is a shift from the optimization of the abiotic components, e.g. the chip, to the improvement of the processing capabilities of cells through genetic engineering. The synthetic biology approach will not only give rise to systems with new functionalities, but will also improve the robustness and speed of their response towards applied signals. To this end, the development of new genetic circuits has to be guided by computational design methods that enable to tune and optimize the circuit response. As the successful design of genetic circuits is highly dependent on the quality and reliability of its composing elements, intense characterization of standard biological parts will be crucial for an efficient rational design process in the development of new genetic circuits. Microengineered devices can thereby offer a new analytical approach for the study of complex biological parts and systems. By summarizing the recent techniques in creating new synthetic circuits and in integrating biology with microdevices, this review aims at emphasizing the power of combining synthetic biology with microfluidics and microelectronics.

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“…ET from biofilms to a solid electrode 10 . Gene deletion studies provide useful information on the roles of specific proteins in the ET process but gaps remain in knowledge of the overall physiology of G. sulfurreducens electron-conducting biofilms.…”

Section: Introductionmentioning

confidence: 99%

Comparative Proteomics Implicates a Role for Multiple Secretion Systems in Electrode-Respiring Geobacter sulfurreducens Biofilms

et al. 2016

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Abstract.Geobacter sulfurreducens is a dissimilatory metal-reducing bacterium capable of forming thick electron-conducting biofilms on solid electrodes. Here, we employ for the first time comparative proteomics to identify key physiological changes involved in G. sulfurreducens adaptation from fumarate-respiring planktonic cells to electron-conducting biofilms.Increased levels of proteins involved in outer membrane biogenesis, cell motility and secretion are expressed in biofilms. Of particular importance to the electron-conducting biofilms are proteins associated with secretion systems of Type I, II, V and Type IV pili.Furthermore, enzymes involved in lipopolysaccharide and peptidoglycan biosynthesis show increased levels of expression in electron-conducting biofilms compared to planktonic cells.These observations point to similarities in long-range electron transfer mechanisms between G. sulfurreducens and Shewanella oneidensis, while highlighting the wider significance of secretion systems beyond that of Type IV pili identified to date in the adaptation of G.sulfurreducens to electrode respiration.

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Insights into genes involved in electricity generation in Geobacter sulfurreducens via whole genome microarray analysis of the OmcF-deficient mutant

Cited by 93 publications

References 45 publications

Identification of Genes Involved in Biofilm Formation and Respiration via Mini- Himar Transposon Mutagenesis of Geobacter sulfurreducens

Identification of Genes Involved in Biofilm Formation and Respiration via Mini- Himar Transposon Mutagenesis of Geobacter sulfurreducens

Synthetic Biology and Microdevices

Comparative Proteomics Implicates a Role for Multiple Secretion Systems in Electrode-Respiring Geobacter sulfurreducens Biofilms

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